Immunosuppresive Effects of the Methanolic Extract of Chrysophyllum Cainito Leaves Onmacrophage Functions

Chan‐Zapata, Iván; Canul-Canche, Jaqueline; Fernández-Martín, Karla; Martín-Quintal, Zhelmy; Torres‐Romero, Julio César; Coral-Martínez, Tania Isolina; Lara-Riegosand, Julio Cesar; Ramírez-Camacho, Mario Alberto

doi:10.21010/ajtcam.v14i1.20

Cited by 5 publications

(3 citation statements)

References 20 publications

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“…The isolation of peritoneal murine macrophages was carried out according to the work by Arana-Argaez et al 18 Briefly, Balb/c mice were sacrificed by the method of cervical dislocation, and then 10 mL of cold phosphate-buffered saline (PBS) was injected into the peritoneal cavity. The macrophages were collected by aspiration of the peritoneal fluid and centrifugation for 10 min at 3,000 rpm and 4°C.…”

Section: Isolation Of Peritoneal Murine Macrophagesmentioning

confidence: 99%

In vitro and in vivo anti-inflammatory properties of Mayan propolis

et al. 2020

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Introduction Propolis has been used traditionally for different human diseases and even recently as dental biomaterials because of its antibacterial, antimycotic, and anti-inflammatory properties. However, a proper correlation between in vitro and in vivo anti-inflammatory properties has not been clearly established. Methods The composition of propolis was determined by high-performance liquid chromatography–ultraviolet mass spectrometry (HPLC-UV-MS). Viability of ethanolic propolis solution was evaluated by thiazolyl blue tetrazolium bromide (MTT) assay on murine macrophages. The anti-inflammatory properties were assessed both in vitro through the enzyme-linked immunosorbent assay (ELISA) quantification of various cytokines and in vivo by induced edemas. Results Chemical analysis showed pinocembrin, pinobanksin-3-O-acetate, and pinobanksin-3-O-propionate as the main components of propolis. Macrophage viability was high (106%) when propolis was used up to 50 µg/mL. ELISA studies showed a reduction in the expression of pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α) up to 145 pg/mL, 350 pg/mL, and 210 pg/mL, respectively, while the anti-inflammatory cytokines (IL-10 and IL-4) were increased up to 833 pg/mL and 446 pg/mL. Finally, edema was reduced on paw and ear mice by 9% and 22%, respectively. Conclusion Mayan propolis has strong in vitro anti-inflammatory properties without compromising macrophage viability, resulting in a low-to-mild in vivo anti-inflammatory response.

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Section: Isolation Of Peritoneal Murine Macrophagesmentioning

confidence: 99%

In vitro and in vivo anti-inflammatory properties of Mayan propolis

et al. 2020

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“…The peritoneal murine macrophages were isolated according to a standard protocol described previously by Arana-Argáez et al (2016). The mice were sacrificed by the cervical dislocation method and peritoneal macrophages were isolated by lavages of the peritoneal cavity with cold phosphate buffered saline (PBS).…”

Section: Isolation and Treatment Of Peritoneal Murine Macrophagesmentioning

confidence: 99%

Immunomodulatory effects of the methanolic extract from Pouteria campechiana leaves in macrophage functions

Chan‐Zapata

Canul-Canche

Fernández-Martín

et al. 2017

Food and Agricultural Immunology

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The present study aimed to examine the immunomodulatory properties of the methanolic (MeOH) extract from Pouteria. campechiana leaves in peritoneal macrophages of Balb/c mice. Peritoneal macrophages isolated from mice and Vero cells were treated with the MeOH extract from leaves. Cell viability of the macrophages and Vero cells were evaluated by the 3-(4,5dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide method. The phagocytic activity, as nitric oxide (NO), hydrogen peroxide (H 2 O 2 ), interleukin 6 (IL-6) and tumour necrosis factor α (TNF-α) production were evaluated on peritoneal macrophages. Results showed that the MeOH extract from leaves was able to stimulate the phagocytic activity and increase NO, H 2 O 2 and cytokines production. The viability assays do not show cytotoxic effect on cell viability and cause a significative proliferative effect in the macrophages of a concentration-dependent manner. These results conclude that the MeOH extract from P. campechiana leaves possessed a stronger immunostimulatory effect in a concentration-dependent manner without affect the cell viability.

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“…We also examined whether recombinant TvTRPV induces macrophages to secrete proinflammatory cytokines by antigen stimulation peritoneal macrophages (MØs), which were isolated from mice as described previously [16]. MØs were seeded at 1×10 5 cells/ml/well in 24-well plate and incubated with recombinant TvTRPV protein at 1, 10, and 100 μg/ml in complete medium (DMEM supplemented with 10% FBS and 1% penicillin-streptomycin) for 48 hr (37°C, 5% CO 2 ).…”

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confidence: 99%

Immune Response of BALB/c Mice toward Putative Calcium Transporter Recombinant Protein of Trichomonas vaginalis

et al. 2019

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Trichomoniasis is a common sexually transmitted infection caused by Trichomonas vaginalis , which actually does not exist a vaccine for control or prevention. Thus, the identification of new and potent immunogens in T. vaginalis , which can contribute to the development of a vaccine against this parasite, is necessary. Therefore, the aim of this work was to evaluate the potential of a recombinant Transient Receptor Potential-like channel of T. vaginalis (TvTRPV), as a promising immunogen in BALB/c mice. First, TvTRPV was cloned and expressed as a recombinant protein in Escherichia coli BL21 cells and purified by nickel affinity. Next, BALB/c mice were immunized and the antibody levels in mice serum and cytokines from the supernatant of macrophages and from co-culture systems were evaluated. Recombinant TvTRPV triggered high levels of specific total IgG in sera from the immunized mice. Also, a statistically significant increase of cytokines: IL-1β, IL-6, and TNF-α after stimulation with the corresponding antigens in vitro, was identified. Moreover, co-cultures using CD4 + T cells from immunized mice were able to identify higher levels of IL-10 and IFN-γ. These results were useful to validate the immunogenicity of TvTRPV in BALB/c mice, where IL-10-IFN-γ-secreting cells could play a role in infection control, supporting the potential of TvTRPV as a promising target for vaccine against T. vaginalis .

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Immunosuppresive Effects of the Methanolic Extract of Chrysophyllum Cainito Leaves Onmacrophage Functions

Cited by 5 publications

References 20 publications

In vitro and in vivo anti-inflammatory properties of Mayan propolis

In vitro and in vivo anti-inflammatory properties of Mayan propolis

Immunomodulatory effects of the methanolic extract from Pouteria campechiana leaves in macrophage functions

Immune Response of BALB/c Mice toward Putative Calcium Transporter Recombinant Protein of Trichomonas vaginalis

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