Immunostimulatory effects of fermented vegetable product on the non-specific immunity of Japanese flounder Paralichthys olivaceus

Abstract

The stimulatory effect of fermented vegetable product (FVP) upon the phagocytic and superoxide generation of leukocytes was studied in the Japanese flounder Paralichthys olivaceus . The phagocytic activity of casein-induced, intraperitoneal leukocytes was investigated and quantified, that is the activity significantly increased ( P < 0.05 or < 0.01) by the addition of FVP beyond 3 mg/kg body weight. Further analysis investigated the effect of FVP on superoxide generation in leukocytes. Established in vitro cytochrome c reduction assay was used to measure superoxide generation; reduced levels of FVP in assay samples had a profound effect on superoxide generation. FVP was also incorporated in commercial diets and fed to Japanese flounder for 4 weeks. The phagocytic activities and superoxide generation of peritoneal induced leukocytes were significantly higher ( P < 0.05, < 0.01) in fish fed the FVP supplemented diet than fish fed the control diet. FVP feeding in fish had a significantly higher ( P < 0.05) activity of lysozyme than in the control fish.KEY WORDS: fermented vegetable product, immunostimulation, Japanese flounder, nonspecific immunity.

Immunostimulant effects of Cotinus coggyria on rainbow trout (Oncorhynchus mykiss)

“…Extracellular respiratory burst activity was enhanced by feeding with aqueous extract of powdered ginger roots in rainbow trout [4]. Respiratory burst was enhanced in Paralichthys olivaceus by dietary fermented vegetable products [24].…”

Molecular characterization, phylogeny, and expression pattern of c-type lysozyme in kelp grouper, Epinephelus bruneus

“…Lysozymes are key components of the innate immune system that catalyze the hydrolysis of bacterial cell walls [3]; they are also useful markers to study the effect of environmental contaminants [35e37], immunostimulants [38,39] as well as bacterial pathogens [9,40] on the immune response in fish. In the present study, we The accession numbers are given as in Table 1.…”

Molecular characterization, phylogeny, and expression of c-type and g-type lysozymes in brill (Scophthalmus rhombus)

“…Lysozymes are key components of the innate immune system that catalyze the hydrolysis of bacterial cell walls [2]. They have revealed as useful markers to study the effect of environmental contaminants [39e41], immunostimulants [42,43] as well as bacterial pathogens [33,44] on the immune response in flatfish. In the present study, we have identified two different lysozyme genes in brill.…”