Immunostimulatory Effects of Extract from &lt;i&gt;Nori&lt;/i&gt; (&lt;i&gt;Porphyra yezoensis&lt;/i&gt;) &lt;i&gt;in Vitro&lt;/i&gt; and &lt;i&gt;in Vivo&lt;/i&gt;

Kitajima, Hideki; Komizu, Yuji; Ichihara, Hideaki; Goto, Kōichi; Doi, Takashi; Inano, Tatsuro; Ueoka, Ryuichi

doi:10.1252/kakoronbunshu.39.359

Cited by 2 publications

(1 citation statement)

References 12 publications

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“…Edible P. yezoensis, commonly referred to as nori, 1 has become a promising source of nutrients, owing to its richness in polysaccharides, which have antioxidative, [2][3][4][5] anti-inflammatory, 6,7 hyperglycemic, 8 and immunostimulatory effects. 9 More recently, several studies have revealed that sulfated polysaccharides from P. yezoensis can inhibit tumor growth both in vitro and in vivo. 10,11 However, despite its biological and pharmacological functions, little attention has been paid to P. yezoensis polysaccharide (PPS) as a functional material for gene transfection.…”

Section: Introductionmentioning

confidence: 99%

Efficient gene delivery to human umbilical cord mesenchymal stem cells by cationized Porphyra yezoensis polysaccharide nanoparticles

Yu¹,

Cao²,

Chen³

et al. 2015

IJN

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This study centered on an innovative application of Porphyra yezoensis polysaccharide (PPS) with cationic modification as a safe and efficient nonviral gene vector to deliver a plasmid encoding human Wnt3a (pWnt3a) into human umbilical cord mesenchymal stem cells (HUMSCs). After modification with branched low-molecular-weight (1,200 Da) polyethylenimine, the cationized PPS (CPPS) was combined with pWnt3a to form spherical nanoscale particles (CPPS-pWnt3a nanoparticles). Particle size and distribution indicated that the CPPS-pWnt3a nanoparticles at a CPPS:pWnt3a weight ratio of 40:1 might be a potential candidate for DNA plasmid transfection. A cytotoxicity assay demonstrated that the nanoparticles prepared at a CPPS:pWnt3a weight ratio of 40:1 were nontoxic to HUMSCs compared to those of Lipofectamine 2000 and polyethylenimine (25 kDa). These nanoparticles were further transfected to HUMSCs. Western blotting demonstrated that the nanoparticles (CPPS:pWnt3a weight ratio 40:1) had the greatest transfection efficiency in HUMSCs, which was significantly higher than that of Lipofectamine 2000; however, when the CPPS:pWnt3a weight ratio was increased to 80:1, the nanoparticle-treated group showed no obvious improvement in translation efficiency over Lipofectamine 2000. Therefore, CPPS, a novel cationic polysaccharide derived from P . yezoensis , could be developed into a safe, efficient, nonviral gene vector in a gene-delivery system.

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