Immunostimulation of Murine Spleen Cells by Materials Associated with Bovine Milk Protein Fractions

Wong, K; Middleton, Natalie; Montgomery, Mitzi; Dey, Moul; Carr, Ronald I.

doi:10.3168/jds.s0022-0302(98)75752-2

Cited by 54 publications

(48 citation statements)

References 19 publications

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“…Mahmud, Matn, and Otani (2004) also found that purified b-LG induces proliferation of mouse resting spleen cells and that mitogenic activity is retained following reduction of the protein, suggesting that the activity depends on the primary structure. However, in contrast to the previous study (Wong et al, 1998), they reported that the mitogenic activity of b-LG is stronger following a 3 h digestion with pepsin, trypsin, chymotrypsin or pancreatin, supporting the view that peptides may be responsible for this activity. In addition, they showed that the most mitogenic pancreatic H induces significant growth of a human B cell line (U266), but does not influence the growth of other cell lines (Ball human B cells, MOLT-4 and Jurkat T cells).…”

Section: Lymphocyte Activation and Proliferationmentioning

confidence: 47%

Immunomodulatory peptides obtained by the enzymatic hydrolysis of whey proteins

Gauthier

Pouliot

Saint-Sauveur

2006

International Dairy Journal

239

121

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Section: Lymphocyte Activation and Proliferationmentioning

confidence: 47%

Immunomodulatory peptides obtained by the enzymatic hydrolysis of whey proteins

Gauthier

Pouliot

Saint-Sauveur

2006

International Dairy Journal

239

121

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“…Several in vitro studies involving murine material have demonstrated immunomodulatory effects of bovine WP preparations on splenic lymphocyte clonal expansion and function. Wong et al (1998) provided evidence that a WP mixture can significantly increase cell expansion and production of IgM by splenocytes. Mercier et al (2004) found whey protein from isolates to significantly induce proliferation of splenic lymphocytes without mitogen stimulation.…”

Section: Discussionmentioning

confidence: 99%

The effects of dietary whey protein concentrate level on performance, selected intestinal tract and blood parameters, and thiobarbituric acid reactive substances in the liver and breast meat of broiler chickens

Szczurek¹,

Szymczyk²,

Arczewska-Włosek³

et al. 2013

J. Anim. Feed Sci.

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ABSTRACT. The aim of this study was to investigate the effect of two levels of whey protein concentrate (WPC, 80.35% protein, 5.86% lactose) added to diets and duration of these treatments on growth and slaughter value, haematological parameters involved in immune processes, oxidative status of liver and breast muscle, and selected indices of gut function of broilers. A total of 560 Ross 308 broilers were randomly allocated into 7 groups with 10 replicates of 8 chickens per replicate. The experiment lasted 42 days and was divided into 3 successive feeding phases. The control group was fed basal diets consisted of maize, wheat and soyabean meal (C group). The other 6 groups received, for 7, 21 and 42 days basal diets with 8 or 32 g · kg -1 WPC added at the expense of soyabean meal (WL and WH groups, respectively). On day 42 group WH had a higher lymphocyte percentage (P < 0.05) compared to the control. The long-term (42 days) feeding with higher level of WPC significantly decreased the heterophile/lymphocyte ratio (P < 0.05 vs C). The number of erythrocytes on day 42 and the whole blood glucose on day 21 were significantly increased in WH birds than in controls. The concentration of serum total protein on both WPC levels was greater (P < 0.05) on day 21 compared with the C group. The reduction (P < 0.05) in liver thiobarbituric acid reactive substances (TBARS) and a tendency towards lower TBARS level in breast meat were observed in birds WH when fed for 42 days. Only higher dietary WPC amount provided for 42 days reduced the caecal and small intestinal pH values (P < 0.05 vs C). The ileal digestibility of crude protein was higher (P < 0.01) in birds fed with 32 g WPC during the first 21 days of life, compared to the C and WL treatments. The factorial ANOVA showed that on days 7, 21 and 42 the final body weight gain (BGW) and feed conversion ratio in broilers WL and WH were significantly better than in the C ones. Assessment of orthogonal contrasts revealed significantly higher (P < 0.05) final BWG in the WH broilers, compared to the WL dietary treatment; such effect was not observed in earlier feeding stages. Generally, carcass yield on both WPC dietary levels, and breast muscle percentage on WH treatment, were higher (P < 0.01 and P < 0.05, respectively) compare to the C group. In conclusion, WPC added to a conventional diets for growing chickens exerts a growth-promoting action and at a dose of 32 g · kg -1 may induce desirable changes in bird's health and the intestinal tract metabolism. W. Szczurek et al. 343

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“…Beta-globulins have been suggested to be activators of PMN functions [33] and their pattern could be related to the RB pattern observed [23].…”

Section: Discussionmentioning

confidence: 99%

The evaluation of non-specific immune status of heifers in field conditions during the periparturient period

et al. 2004

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-During the periparturient period, some impairment of immune defences were observed. Reference values for the different non-specific immune parameters in cows are not generally available, thus limiting the application of these parameters in dairy practice. This paper reports the data on the measurements of different parameters in the blood, and explores the possible influence of the herd on the non-specific immune status of the cow. Five herds located in Northern Italy were selected and overall 39 heifers were enrolled in the trial. Blood samples were taken 14 and 7 days before the expected date of calving, then at 7, 14, 21, 28, 45, 60, and 75 days after calving. The parameters assessed were N-acetyl-β-glucosaminidase (NAGase), lysozyme, nitric oxide, superoxide dismutase, haptoglobin, respiratory burst, and serum protein profile. After calving, a significant decrease of respiratory burst and nitric oxide concentration were observed in comparison with the pre-calving values but not with the post-calving samplings. Total proteins, β-and γ-globulins showed a progressive and significant increase in concentration after calving, in comparison with pre-calving values. The results of the study confirmed that a decrease of immune functions can be observed in commercial dairy herds in the first four weeks after calving. The amplitude of this phenomenon is not common to all animals and all herds, suggesting the possibility to reduce the impairment by improved management and genetic selection. heifers / periparturient period / blood / non-specific immunity

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Immunostimulation of Murine Spleen Cells by Materials Associated with Bovine Milk Protein Fractions

Cited by 54 publications

References 19 publications

Immunomodulatory peptides obtained by the enzymatic hydrolysis of whey proteins

Immunomodulatory peptides obtained by the enzymatic hydrolysis of whey proteins

The effects of dietary whey protein concentrate level on performance, selected intestinal tract and blood parameters, and thiobarbituric acid reactive substances in the liver and breast meat of broiler chickens

The evaluation of non-specific immune status of heifers in field conditions during the periparturient period

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