Immunostimulation by poly-β hydroxybutyrate–hydroxyvalerate (PHB–HV) from Bacillus thuringiensis in Oreochromis mossambicus

Ponnusamy, S.; Binuramesh, C.; Abirami, Periyasamy; Viswanathan, S.; Poornima, Kkani; Rajeswari, V.; Shenbagarathai, R.

doi:10.1016/j.fsi.2013.10.012

Cited by 67 publications

(47 citation statements)

References 55 publications

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“…Defoirdt et al [28] reported that short-chain fatty acids like PHB showed a strong growth-inhibitory against pathogenic V. campbellii . Recently PHB was proven to be an immunostimulant in fish [29]. The synergistic effect of PHB-degrading probiotic and PHB as a synbioticum elicits a strong protection against luminescent vibriosis [30].…”

Section: Discussionmentioning

confidence: 99%

Antiadhesive activity of poly-hydroxy butyrate biopolymer from a marine Brevibacterium casei MSI04 against shrimp pathogenic vibrios

et al. 2014

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BackgroundVibrio pathogens are causative agents of mid-culture outbreaks, and early mortality syndrome and secondary aetiology of most dreadful viral outbreaks in shrimp aquaculture. Among the pathogenic vibrios group, Vibrio alginolyticus and V. harveyi are considered as the most significant ones in the grow-out ponds of giant black tiger shrimp Penaeus monodon in India. Use of antibiotics was banned in many countries due to the emergence of antibiotic-resistant strains and accumulation of residual antibiotics in harvested shrimp. There is an urgent need to consider the use of alternative antibiotics for the control of vibriosis in shrimp aquaculture. Biofilm formation is a pathogenic and/or establishment mechanism of Vibrio spp. This study aims to develop novel safe antibiofilm and/or antiadhesive process using PHB to contain vibrios outbreaks in shrimp aquaculture.ResultsIn this study a poly-hydroxy butyrate (PHB) polymer producing bacterium Brevibacterium casei MSI04 was isolated from a marine sponge Dendrilla nigra and production of PHB was optimized under submerged-fermentation (SmF) conditions. The effect of carbon, nitrogen and mineral sources on PHB production and enhanced production of PHB by response surface methods were demonstrated. The maximum PHB accumulation obtained was 6.74 g/L in the optimized media containing 25 g/L starch as carbon source, 96 h of incubation, 35°C and 3% NaCl. The highest antiadhesive activity upto 96% was recorded against V. vulnificus, and V. fischeri, followed by 92% against V. parahaemolyticus and V. alginolyticus and 88% inhibition was recorded against V. harveyi.ConclusionIn this study, a thermostable biopolymer was chemically characterized as PHB based on 1HNMR spectra, FT-IR and GC-MS spectra. The NMR spectra revealed that the polymer was an isocratic homopolymer and it also confirmed that the compound was PHB. The antiadhesive activity of PHB was determined in microtitre plate assay and an effective concentration (EC) of PHB (200 μl containing 0.6 mg PHB) was confirmed by confocal laser scanning microscopic analysis of vibrio biofilm on pre-treated glass and polystyrene surfaces. This is a first report on anti-adhesive activity of PHB against prominent vibrio pathogens in shrimp aquaculture.Electronic supplementary materialThe online version of this article (doi:10.1186/s12934-014-0114-3) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Antiadhesive activity of poly-hydroxy butyrate biopolymer from a marine Brevibacterium casei MSI04 against shrimp pathogenic vibrios

et al. 2014

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“…The cause for the above observation is unclear; however, one may argue that dietary MOS exerts its protective effects by modulating other machineries (e.g., phagocytic activities, antioxidant activities; Merrifield et al ) of the defense system. The effects of dietary PHB on the generation of protective immune responses against pathogenic bacteria system have been well reported in different aquatic species (Baruah et al ; Laranja et al ; Nhan et al ; Suguna et al ; Sui et al ). Degradation of PHB by enzymatic and/or microbial activity in the GI tract of the organisms results in the release of β‐hydroxybutyric acid or PHB oligomers (Defoirdt et al ).…”

Section: Discussionmentioning

confidence: 99%

Evaluating the Effects of Dietary Prebiotic Mixture of Mannan Oligosaccharide and Poly‐β‐Hydroxybutyrate on the Growth Performance, Immunity, and Survival of Rainbow Trout, Oncorhynchus mykiss (Walbaum 1792), Fingerlings

Najdegerami

Tokmachi

Bakhshi

2016

J World Aquaculture Soc

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This study investigated the effects of dietary poly‐β‐hydroxybutyrate (PHB) and mannan oligosaccharide (MOS) on the growth performance, digestive enzyme activities, and disease resistance of rainbow trout fingerlings. A total of 420 fingerlings, distributed equally into four different groups, each with three replicates, were fed a basal diet (control), 2% PHB (PHB), 0.2% MOS (MOS), or a diet supplemented with a mixture of 2% PHB and 0.2% MOS (PHB+MOS) for 60 d. Results showed that dietary PHB and/or MOS did not cause significant improvement in the growth performance of the fingerlings. The gut pH was markedly reduced; however, the activities of the digestive enzymes (except for pepsin and amylase by dietary PHB) were not significantly improved by the dietary supplements. Results also showed that dietary PHB and/or MOS markedly increased the resistance of the fingerlings toward Yersinia ruckeri infection, while the lowest total antibody was observed in fish fed control and MOS, indicating that MOS exerts its protective effects by modulating other mechanisms. Overall, this study provided a first screening effort to determine the effects of PHB and/or MOS as dietary supplements in rainbow trout fingerlings to develop an optimal prebiotic mix for improving the growth and health status of rainbow trout.

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“…For instance, PHB produced by Bacteroides thuringensis and delivered orally to Nile tilapia increases serum antibodies as well as innate immune parameters (52). However, how the PHB send this message to the systemic immune system is not understood.…”

Section: Interactions Between Microbiota and The Teleost Systemic Immmentioning

confidence: 99%

Under Pressure: Interactions between Commensal Microbiota and the Teleost Immune System

2017

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Commensal microorganisms inhabit every mucosal surface of teleost fish. At these surfaces, microorganisms directly and indirectly shape the teleost immune system. This review provides a comprehensive overview of how the microbiota and microbiota-derived products influence both the mucosal and systemic immune system of fish. The cross talk between the microbiota and the teleost immune system shifts significantly under stress or disease scenarios rendering commensals into opportunists or pathogens. Lessons learnt from germ-free fish models as well as from oral administration of live probiotics to fish highlight the vast impact that microbiota have on immune development, antibody production, mucosal homeostasis, and resistance to stress. Future studies should dissect the specific mechanisms by which different members of the fish microbiota and the metabolites they produce interact with pathogens, with other commensals, and with the teleost immune system.

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Immunostimulation by poly-β hydroxybutyrate–hydroxyvalerate (PHB–HV) from Bacillus thuringiensis in Oreochromis mossambicus

Cited by 67 publications

References 55 publications

Antiadhesive activity of poly-hydroxy butyrate biopolymer from a marine Brevibacterium casei MSI04 against shrimp pathogenic vibrios

Antiadhesive activity of poly-hydroxy butyrate biopolymer from a marine Brevibacterium casei MSI04 against shrimp pathogenic vibrios

Evaluating the Effects of Dietary Prebiotic Mixture of Mannan Oligosaccharide and Poly‐β‐Hydroxybutyrate on the Growth Performance, Immunity, and Survival of Rainbow Trout, Oncorhynchus mykiss (Walbaum 1792), Fingerlings

Under Pressure: Interactions between Commensal Microbiota and the Teleost Immune System

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