Immunostimulant potential of Moringa Oleifera leaves alcoholic extract versus Oregano Essential Oil (OEO) against cyclophosphamide-induced immunosuppression in broilers chicks

Abstract

The current study was conducted to evaluate the immunoenhancement effect of Moringa oleifera leaves alcoholic extract (MOLE) versus Oregano essential oil (OEO) against cyclophosphamide induced immunosuppression in broilers chicks. A total of a three hundred one-day-old chicks were assigned randomly into three main dietary groups, control, MOLE, and OEO for 14 days. After 14 days the three main experimental groups were subdivided into six groups, control, cyclophosphamide, MOLE, MOLE and Cyclophosphamide, OEO, and OEO and cyclophosphamide. Each group of these six groups was subdivided into three subgroups. Supplementation of broiler chicks with MOLE and OEO for 14 days significantly increased body weight compared to the control group. However, injection of broiler chicks with cyclophosphamide significantly induced body weight loss, impaired immunological response represented by decreasing total leukocytic count, differential leukocytic count, phagocytic activity, phagocytic index, and hemagglutinin inhibition titer for New Castle disease virus, lymphoid organs depletion, and increased the mortality rate. In contrast, supplementation of cyclophosphamide treated chicks with MOLE and OEO significantly reduced cyclophosphamide induced body weight loss and impaired immunological responses, as it showed significant increase in body weight, total leukocytic count, differential leukocytic count, phagocytic activity, phagocytic index, and hemagglutinin inhibition titer for New Castle disease virus, lymphoid organs proliferation, and reduced the mortality rate. This study indicated that MOLE and OEO supplementation ameliorated cyclophosphamide induced body weight loss and impaired immunological responses.

Protective effect of coenzyme Q10 in cyclophosphamide-induced kidney damage in rats

“…Stankiewicz et al, reported that oxidative stress and elevated reactive oxygen species (ROS) could play an important role in cyclophosphamide-induced kidney damage 3 . Furthermore, prior studies demonstrated that cyclophosphamide could inhibit the activities of antioxidant enzymes such as superoxide dismutase (SOD) and catalase (CAT) 4,5 . Other theories related to nephrotoxicity due to cyclophosphamide were imbalance of the oxidants-antioxidants system, increase of the inflammatory cytokines, and apoptosis 6 , although there is no consensus that, to prevent the toxicity due to cyclophosphamide, a potent antioxidant agent could be useful.…”