Immunospecific Regression of Methylcholanthrene Fibrosarcoma Using Neuraminidase

Simmons, Richard L.; Rios, Angelyn

doi:10.1097/00000658-197208000-00011

Cited by 37 publications

(25 citation statements)

References 16 publications

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“…From the viewpoint of tumor immunity, removal of sialic acid from the cell surface causes a reduced growth of tumors in susceptible hosts (48) and increases tumor immunogenicity (4,42). In the present study, 10 U of VCN/ml HBSS was enough to erase the negative charge on the lymphocyte (24) and to reduce the number of ferritin particles on the trophoblastic surface to about one half with slight morphological changes in these cells.…”

Section: Discussionmentioning

confidence: 99%

Ultrastructural Demonstration of the Negative Surface Charge on Human Trophoblast

Kawagoe

Kawana

Sakamoto

1980

Acta Histochem. Cytochem

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Section: Discussionmentioning

confidence: 99%

Ultrastructural Demonstration of the Negative Surface Charge on Human Trophoblast

Kawagoe

Kawana

Sakamoto

1980

Acta Histochem. Cytochem

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“…The use of enzymes in enhancing the serological reactivity of red cells, long an integral part of routine blood typing technique, has been recently joined by the introduction of enzyme methods for histocompatibility testing in which cells show increased reactivity with cytotoxic antibodies [19,28], or enhanced stimulation in mixed leukocyte cultures [7,15]. Further interest in enzyme-treated cells has been stimulated by the report of their effectiveness in stimulating the immune rejection of experimental tumors [26,28], In the case of the RBC, proteolytic enzymes [27] as well as neuraminidase [4,6] reduce the red cell surface electrostatic charge by removing free or glycopeptide-bound sialic acid, the principal charged component of the membrane.…”

Section: Introductionmentioning

confidence: 99%

Effects of Proteases and Neuraminidase on RBC Surface Charge and Agglutination

Luner¹,

Sturgeon²,

Szklarek³

et al. 1975

Vox Sanguinis

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Electrophoretic mobility, membrane sialic acid content and agglutinability by ‘incomplete’ antisera against Rh(0), hr" and k antigens were determined for red blood cells in the course of treatment with trypsin, ficin and neuraminidase. Neuraminidase gradually produced a slight to moderate agglutinability as it reduced surface charge density in proportion to the amount of sialic acid removed. Proteases acted in two distinct steps. The first stage is characterized by the cells rapidly becoming highly agglutinable and by the unmasking of new negative charge as the first half of the sialic acid is removed. In the second stage the cells show a slight gain in agglutinability as surface charge is removed in proportion to sialic acid removal as in the case of neuraminidase. Neuraminidase-treated cells are considerably less agglutinable than cells reduced to the same ξ-potential by protease treatment. The greater efficacy of proteases compared to neuraminidase in making cells agglutinable could be because they not only reduce surface charge density but also increase antigen-antibody bond strength, render antigens more mobile in the membrane to allow clustering in regions of cell to cell antibody bridging and remove glycopeptide chains which may be causing steric hindrance to antigenantibody binding or to cell-cell contact.

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“…Serum (Simmons & Rios, 1974;Simmons et al, 1971 ;Reed et al, 1974). The changes shown to occur in the metabolism of tumour-cell sialoglvcoproteins (Warren et al, 1972) (Silver et al, 1978) and of 4 patients with metastatic breast cancer (Macbeth & Bekesi, 1962 Stage IV Group consisted of 19 patients.…”

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confidence: 99%