Immunospecific ferromagnetic iron-dextran reagents for the labeling and magnetic separation of cells

Molday, Robert S.; MacKenzie, Donald J.

doi:10.1016/0022-1759(82)90007-2

Cited by 579 publications

(290 citation statements)

References 21 publications

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“…87,88,92,93,121 This synthesis approach can be conducted either in the absence or presence of surface complexing agents, such as dextran, polyethylene glycol (PEG), or Table 2. Bare SPIO (i.e., without surface complexing agents) are created following the addition of a concentrated base to a di-and trivalent ferrous salt solution.…”

Section: Coprecipitationmentioning

confidence: 99%

“…85 The hydroxyl surface groups can be modified to display functional amines and carboxyls, relevant for further bioconjugation 56 and the silica itself can be dyedoped. 85,114 As mentioned earlier, SPIO can also be formed directly in the presence of a coating polymer, usually dextran, 93,156 although a variety of coating systems are available (Table 2). 92,102 As with the bare SPIO, alkaline agents are used to coprecipitate the di-and trivalent ferrous salts, but here these salts have been dissolved directly in the presence of dextran.…”

Section: Coprecipitationmentioning

confidence: 99%

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Superparamagnetic Iron Oxide Nanoparticle Probes for Molecular Imaging

et al. 2006

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Abstract-The field of molecular imaging has recently seen rapid advances in the development of novel contrast agents and the implementation of insightful approaches to monitor biological processes non-invasively. In particular, superparamagnetic iron oxide nanoparticles (SPIO) have demonstrated their utility as an important tool for enhancing magnetic resonance contrast, allowing researchers to monitor not only anatomical changes, but physiological and molecular changes as well. Applications have ranged from detecting inflammatory diseases via the accumulation of non-targeted SPIO in infiltrating macrophages to the specific identification of cell surface markers expressed on tumors. In this article, we attempt to illustrate the broad utility of SPIO in molecular imaging, including some of the recent developments, such as the transformation of SPIO into an activatable probe termed the magnetic relaxation switch.

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Section: Coprecipitationmentioning

confidence: 99%

Section: Coprecipitationmentioning

confidence: 99%

Superparamagnetic Iron Oxide Nanoparticle Probes for Molecular Imaging

et al. 2006

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“…Monoclonal antibody A7 was coupled with the dextran of Ferumoxides using a modification of the sodium periodate method (Molday and Mackenzie, 1982). In all, 1 ml of Ferumoxides was added to 10 mM sodium periodate.…”

Section: Mab A7 Coupled To Ferumoxidesmentioning

confidence: 99%

Monoclonal antibody A7-superparamagnetic iron oxide as contrast agent of MR imaging of rectal carcinoma

et al. 2005

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Superparamagnetic iron oxide (SPIO)-based colloid has been used clinically as a tissue-specific magnetic resonance contrast agent. We coupled monoclonal antibody A7 (Mab A7), which reacts specifically with human colorectal carcinoma, to Ferumoxides (SPIO) and examined the accumulation of this conjugate in xenografted tumours in nude mice. We examined in vitro immunoreactivity of Mab A7 coupled to Ferumoxides and its in vivo distribution in nude mice with human colorectal carcinoma. Magnetic resonance imaging of tumour-bearing nude mice was performed 72 h after injection of A7-Ferumoxides. A7-Ferumoxides retained binding activities that were nearly identical to intact Mab A7. More of the radiolabelled A7-Ferumoxides accumulated in the tumour than normal mouse IgG-Ferumoxides from 12 h onwards after injection (Po0.05). Both A7-Ferumoxides and normal mouse IgGFerumoxides disappeared from blood linearly over time. The accumulation levels in normal tissue decreased linearly over time but were lower than levels in tumours from 6 h. In magnetic resonance T2-weighted imaging of the tumour-bearing nude mice, signal intensity was reduced at the margin of the tumour by injection of A7-Ferumoxides. Mab A7 coupled to Ferumoxides is potentially suitable as a magnetic resonance contrast agent for detecting local recurrence of rectal carcinoma.

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“…Nanoparticles can be used for tumor destruction via heating (hyperthermia) [75] . They help in the parting and refinement of molecules and cells [76] , MRI contrast enhancement [77] and phagokinetic studies [78] . Other applications regarding manufacturing and materials, energy and electronics are: Carbon tetrachloride trash in water can be cleaned up by using iron nanoparticles.…”

Section: Synthesis Of Nanoparticles From Plantsmentioning

confidence: 99%

A Brief Review on Nanoparticles: Types of Platforms, Biological Synthesis and Applications

Anwar¹

2018

Res. Rev. J Mat. Sci

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Immunospecific ferromagnetic iron-dextran reagents for the labeling and magnetic separation of cells

Cited by 579 publications

References 21 publications

Superparamagnetic Iron Oxide Nanoparticle Probes for Molecular Imaging

Superparamagnetic Iron Oxide Nanoparticle Probes for Molecular Imaging

Monoclonal antibody A7-superparamagnetic iron oxide as contrast agent of MR imaging of rectal carcinoma

A Brief Review on Nanoparticles: Types of Platforms, Biological Synthesis and Applications

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