Immunosensors made of polymer-infused porous paper for the non-invasive detection of airways cytokines trapped by porous face masks

Vaquer, Andreu; Adrover-Jaume, Cristina; Clemente, Antonio; Iglesias, Amanda; López, Meritxell; Martínez, Rocío Anguita; Roig, Isabel María; Cosío, Borja G.; Rica, Roberto de la

doi:10.1016/j.snb.2022.133233

Cited by 4 publications

(5 citation statements)

References 29 publications

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“…The most outstanding feature of the microfluidic system is its ability to analyze and manipulate microliters of fluid in channels with diameters of 10–100 μm [ 41 , 42 ]. A porous structured microfluidic system with small thermal mass, high mass transfer efficiency, high specific surface area, good permeability, low relative density and high specific strength can effectively improve the flux rate and sensitivity of the device [ 43 , 44 , 45 , 46 , 47 , 48 , 49 ]. The diversity of porous materials stems from a wide range of preparation methods [ 50 , 51 , 52 , 53 , 54 ].…”

Section: Different Materials and Preparation Methods For Microfluidic...mentioning

confidence: 99%

Porous Structural Microfluidic Device for Biomedical Diagnosis: A Review

et al. 2023

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Microfluidics has recently received more and more attention in applications such as biomedical, chemical and medicine. With the development of microelectronics technology as well as material science in recent years, microfluidic devices have made great progress. Porous structures as a discontinuous medium in which the special flow phenomena of fluids lead to their potential and special applications in microfluidics offer a unique way to develop completely new microfluidic chips. In this article, we firstly introduce the fabrication methods for porous structures of different materials. Then, the physical effects of microfluid flow in porous media and their related physical models are discussed. Finally, the state-of-the-art porous microfluidic chips and their applications in biomedicine are summarized, and we present the current problems and future directions in this field.

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Section: Different Materials and Preparation Methods For Microfluidic...mentioning

confidence: 99%

Porous Structural Microfluidic Device for Biomedical Diagnosis: A Review

et al. 2023

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“…Paper biosensors were fabricated following our previously described polymer-infusing method. 18 Briefly, Whatman filter paper #1 was cut into 7 × 7 cm squares. Then, 3 mL of 18% PSS was added to a Petri dish and the paper square was placed above the liquid.…”

Section: Methodsmentioning

confidence: 99%

“…The spray bottle and the polypropylene piece were held by a 3D printed device that maintained a fixed distance between them. 18 Each bacterial suspension was sprayed thrice and, after drying at room temperature (RT), clipped with three layers of blotting filter that had been previously soaked with 1.9 mL of PBS-BSA assembled on a clamp tool. Next, the paper biosensor was placed above the polypropylene piece and pressed against it for 5 min under high pressure.…”

Section: Surgical Facemask Processingmentioning

confidence: 99%

“…After washing away excess nanoprobes, a colored spot remains whose pixel intensity correlates with the target concentration. While this approach has been successfully used to detect proteins (viral antigens, cytokines), 17,18 this is the first report of applying it for detecting bacterial cells. This brought new analytical challenges regarding facemask processing that have been successfully addressed here.…”

Section: Introductionmentioning

confidence: 99%

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Facemask analyses for the non-invasive detection of chronic and acute P. aeruginosa lung infections using nanoparticle-based immunoassays

Delgado-Cano,

Clemente,

Adrover-Jaume

et al. 2023

Analyst

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“…Ab-AuNPs reservoirs were made of filter paper sheets infused with polystyrene sulfonate (PSS) following our already published method [ 27 ]. Briefly, Whatman filter paper #1 was cut into 7 × 7 cm 2 squares.…”

Section: Methodsmentioning

confidence: 99%

A paper biosensor for overcoming matrix effects interfering with the detection of sputum pyocyanin with competitive immunoassays

Adrover-Jaume,

Clemente,

Rodríguez-Urretavizcaya

et al. 2023

Microchim Acta

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Detecting sputum pyocyanin (PYO) with a competitive immunoassay is a promising approach for diagnosing Pseudomonas aeruginosa respiratory infections. However, it is not possible to perform a negative control to evaluate matrix-effects in competitive immunoassays, and the highly complex sputum matrix often interferes with target detection. Here, we show that these issues are alleviated by performing competitive immunoassays with a paper biosensor. The biosensing platform consists of a paper reservoir, which contains antibody-coated gold nanoparticles, and a substrate containing a competing recognition element, which is a piece of paper modified with an albumin-antigen conjugate. Detection of PYO with a limit of detection of 4.7·10−3 µM and a dynamic range between 4.7·10−1 µM and 47.6 µM is accomplished by adding the sample to the substrate with the competing element and pressing the reservoir against it for 5 min. When tested with patient samples, the biosensor was able to qualitatively differentiate spiked from non-spiked samples, whereas ELISA did not show a clear cut-off between them. Furthermore, the relative standard deviation was lower when determining sputum with the paper-based biosensor. These features, along with a mild liquefaction step that circumvents the use of harsh chemicals or instruments, make our biosensor a good candidate for diagnosing Pseudomonas infections at the bedside through the detection of sputum PYO. Graphical Abstract

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Immunosensors made of polymer-infused porous paper for the non-invasive detection of airways cytokines trapped by porous face masks

Cited by 4 publications

References 29 publications

Porous Structural Microfluidic Device for Biomedical Diagnosis: A Review

Porous Structural Microfluidic Device for Biomedical Diagnosis: A Review

Facemask analyses for the non-invasive detection of chronic and acute P. aeruginosa lung infections using nanoparticle-based immunoassays

A paper biosensor for overcoming matrix effects interfering with the detection of sputum pyocyanin with competitive immunoassays

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