Immunosensor for trace penicillin G detection in milk based on supported bilayer lipid membrane modified with gold nanoparticles

Han, Li; Xu, Bo; Danqi, Wang; Zhou, Yifang; Zhang, Hongling; Xia, Wei; Xu, Shunqing; Li, Yuanyuan

doi:10.1016/j.jbiotec.2015.03.013

Cited by 38 publications

(10 citation statements)

References 32 publications

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“…Penicillin biosensing has been achieved by a number of different sensor layouts before. An electrochemical immunosensor employed anti-penicillin G antibodies integrated in a supported bilayer lipid membrane (s-BLM) modified with AuNPs 76 . Binding of penicillin G was detected by impedance spectroscopy, with the AuNPs serving mainly as antibody-stabilizing additives.…”

Section: Discussionmentioning

confidence: 99%

Penicillin Detection by Tobacco Mosaic Virus-Assisted Colorimetric Biosensors

Koch¹,

Poghossian²,

Schöning³

et al. 2018

Nanotheranostics

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The presentation of enzymes on viral scaffolds has beneficial effects such as an increased enzyme loading and a prolonged reusability in comparison to conventional immobilization platforms. Here, we used modified tobacco mosaic virus (TMV) nanorods as enzyme carriers in penicillin G detection for the first time. Penicillinase enzymes were conjugated with streptavidin and coupled to TMV rods by use of a bifunctional biotin-linker. Penicillinase-decorated TMV particles were characterized extensively in halochromic dye-based biosensing. Acidometric analyte detection was performed with bromcresol purple as pH indicator and spectrophotometry. The TMV-assisted sensors exhibited increased enzyme loading and strongly improved reusability, and higher analysis rates compared to layouts without viral adapters. They extended the half-life of the sensors from 4 - 6 days to 5 weeks and thus allowed an at least 8-fold longer use of the sensors. Using a commercial budget-priced penicillinase preparation, a detection limit of 100 µM penicillin was obtained. Initial experiments also indicate that the system may be transferred to label-free detection layouts.

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Section: Discussionmentioning

confidence: 99%

Penicillin Detection by Tobacco Mosaic Virus-Assisted Colorimetric Biosensors

Koch¹,

Poghossian²,

Schöning³

et al. 2018

Nanotheranostics

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“…M [11]. Our prepared PB has the advantage that it allows the determination of penicillin in a wider range and lower concentration.…”

Section: Effect Of Buffer Concentrationmentioning

confidence: 99%

Nanoparticle Based Biosensor for Penicillin Quantification in Pharmaceuticals

Karakuş

Turan

2019

Int J App Pharm

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Objective: The objective of this study was to develop a new biosensor system based on nanoparticle to determine penicillin in pharmaceuticals. Methods: The characterization and optimization of the potentiometric penicillin biosensor (PB) were prepared by using synthesized surface-dependent and surface-independent ZnO nanoparticles named ZnO nanorods and chitosan were carried out. It was preferred ZnO nanorod because of its electrical, optical, physical and photocatalyst properties, biocompatibility and non-toxicity in the construction of the penicillin biosensor. Results: The operating range was obtained as 10-1-10-3M, the optimum buffer concentration was 10 mmol, optimum pH was 7.4 and the optimum temperature was 25 °C for the PB. The PB has advantages in terms of short response time, long enough shelf life, cheap, and easy elaborate. Conclusion: Whether the biosensor can be used to determine penicillin and accurately measure penicillin, the amount of penicillin in a commercial pharmaceutical preparation named Alfoxil was successfully made by using our prepared penicillin biosensor.

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“…122 This large market has resulted in the development of a large variety of electrochemical sensors to assess milk quality for possible contaminants, including melamine, 123,124 antibiotics, 59,125 bisphenol A (from packaging contamination), 126 DNA bases, 127 and hydrogen peroxide. 122,128,129 Hydrogen peroxide has been used as a means of artificially preserving milk 130 and due to the possibility of adverse health effects from over exposure, the US Food and Drug Administration has limited its acceptable concentration to 80 ppm.…”

Section: Food Safetymentioning

confidence: 99%

“…The significant enhancement of electric fields by using submicron spaced IDEs coupled with the label free nature of EIS makes these methods an attractive option compared with more traditional fluorometric techniques. As such, many nanomaterials, and combinations of nanomaterials (nanoparticles, 59,60 nanorods, 61 carbon nanotubes, 62 graphene…”

mentioning

confidence: 99%