Immunosensing prostate-specific antigen: Faradaic vs non-Faradaic electrochemical impedance spectroscopy analysis on interdigitated microelectrode device

Ibau, C.; Arshad, M.K. Md; Gopinath, Subash C.B.; M.N, M. Nuzaihan; Fathil, M. F. M.; Shamsuddin, Shahidah Arina

doi:10.1016/j.ijbiomac.2020.08.125

Cited by 30 publications

(17 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It can conclude that silica nanoparticle surface is porous and dense. Additionally, different silica sizes and shapes are contributed to the incomplete core reaction as a result of varying age and temperature 46 , 47 . Apart from that, nano-sizing of silica nanoparticles was obtained after undergoes with centrifugation processes and nano-filtration procedures 48 .…”

Section: Resultsmentioning

confidence: 99%

“…The I–V values were calculated after the interaction of the immobilized aptamer with As(III). The calibration plot was established and LOD was estimated using a linear regression analysis using the formula: where α is the standard deviation of the biosensing interaction, which was measured from the calibration curve y-residuals, and ‘ b ’ is the biosensor sensitivity, which was calculated from the calibration curve's slope 46 .…”

Section: Experimental Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Silica and graphene mediate arsenic detection in mature rice grain by a newly patterned current–volt aptasensor

Uda

Gopinath

Hashim

et al. 2021

Sci Rep

View full text Add to dashboard Cite

Arsenic is a major global threat to the ecosystem. Here we describe a highly accurate sensing platform using silica nanoparticles/graphene at the surface of aluminum interdigitated electrodes (Al IDE), able to detect trace amounts of arsenic(III) in rice grain samples. The morphology and electrical properties of fabricated Al IDEs were characterized and standardized using AFM, and SEM with EDX analyses. Micrometer scale Al IDEs were fabricated with silicon, aluminum, and oxygen as primary elements. Validation of the bare Al IDE with electrolyte fouling was performed at different pH levels. The sensing surface was stable with no electrolyte fouling at pH 7. Each chemical modification step was monitored with current–volt measurement. The surface chemical bonds were characterized by fourier transform infrared spectroscopy (FTIR) and revealed different peaks when interacting with arsenic (1600–1000 cm−1). Both silica nanoparticles and graphene presented a sensitive limit of detection as measured by slope calibration curves at 0.0000001 pg/ml, respectively. Further, linear regression was established using ΔI (A) = 3.86 E−09 log (Arsenic concentration) [g/ml] + 8.67 E−08 [A] for silica nanoparticles, whereas for graphene Y = 3.73 E−09 (Arsenic concentration) [g/ml] + 8.52 E−08 on the linear range of 0.0000001 pg/ml to 0.01 pg/ml. The R2 for silica (0.96) and that of graphene (0.94) was close to the maximum (1). Modification with silica nanoparticles was highly stable. The potential use of silica nanoparticles in the detection of arsenic in rice grain extract can be attributed to their size and stability.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Experimental Methodsmentioning

confidence: 99%

Silica and graphene mediate arsenic detection in mature rice grain by a newly patterned current–volt aptasensor

Uda

Gopinath

Hashim

et al. 2021

Sci Rep

View full text Add to dashboard Cite

show abstract

“…The final optimization step is to determine the adiponectin incubation time for high interaction of adiponectin and the antiadiponectin solution. For this purpose, the biosensors were prepared in optimum conditions and incubated with adiponectin solutions for three different periods (30,45, and 60 min) and the impedance measurements were performed. R ct values were calculated by the obtained impedance curves, and the calibration graphs (Figure 3C) were drawn.…”

Section: Optimization Study Of the Proposed Biosensormentioning

confidence: 99%

“…The impedance spectroscopy techniques are useful and preferable to accomplish the surface characterization of the electrode and examine the interaction between the biorecognition material and bioanalyte . EIS is an important method for investigating surface morphology with imaging techniques due to these features. − …”

Section: Introductionmentioning

confidence: 99%

Highly Sensitive and Single-Use Biosensing System Based on a GP Electrode for Analysis of Adiponectin, an Obesity Biomarker

Özcan

Sezgintürk

2021

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

This study presents a disposable, novel, and sensitive biosensing system to determine adiponectin, an obesity biomarker, in real human serum. The graphite paper (GP) working electrode is a new material for impedimetric biosensors. In the literature, there is no study in which this electrode is used in impedance-based biosensors for adiponectin detection. Sensitive and useful techniques, such as electrochemical impedance spectroscopy and cyclic voltammetry, were utilized for investigation of the modification of the GP electrode surface and optimization and characterization of the constructed biosensor. The single frequency impedance technique was used to study the interactions between antiadiponectin and adiponectin. The morphology of the electrode surface for each immobilization step was examined with scanning electron microscopy. All experimental parameters were optimized to fabricate a rapid and sensitive biosensing system. The designed biosensor presents excellent performance with a wide detection range (0.05–25 pg mL–1) and a low limit of detection (0.0033 pg mL–1) for adiponectin determination. Also, it has been demonstrated that the biosensor sensitively allows for the detection of adiponectin in human serum. The affinity of the designed immunosensor toward other proteins and components was examined in the presence of the target protein (adiponectin), leptin (100 pg mL–1), creatine kinase (50 pg mL–1), parathyroid hormone (50 pg mL–1), and d-glucose (0.5 M). The selectivity of the adiponectin biosensor resulted in high capacity to neglect the interference effect. The constructed biosensor showed good linearity, long-term storage life (10 weeks), high reusability (18 times regenerability), and high ability to detect adiponectin concentrations at picogram levels.

show abstract

“…The performance of developed aptasensor was determined by Electrochemical Impedance Spectroscopy (EIS) electrical characterization. EIS analyses are well-defined in the recent state-of-art of biosensor, especially in resulting exact impedance spectra and evaluation for precise changes of surface chemistry performed on a sensing surface ( Ibau et al, 2020 ; Krishnan et al, 2021 ). With the regard, the intensity of anti-NCP aptamer and NCP specific binding on diamond enhanced AuIDE was validated through the impedimetric assessment.…”

Section: Introductionmentioning

confidence: 99%

Aptasensing nucleocapsid protein on nanodiamond assembled gold interdigitated electrodes for impedimetric SARS-CoV-2 infectious disease assessment

Ramanathan

Ismail

Arshad

et al. 2022

Biosensors and Bioelectronics

Self Cite

View full text Add to dashboard Cite

Immunosensing prostate-specific antigen: Faradaic vs non-Faradaic electrochemical impedance spectroscopy analysis on interdigitated microelectrode device

Cited by 30 publications

References 30 publications

Silica and graphene mediate arsenic detection in mature rice grain by a newly patterned current–volt aptasensor

Silica and graphene mediate arsenic detection in mature rice grain by a newly patterned current–volt aptasensor

Highly Sensitive and Single-Use Biosensing System Based on a GP Electrode for Analysis of Adiponectin, an Obesity Biomarker

Aptasensing nucleocapsid protein on nanodiamond assembled gold interdigitated electrodes for impedimetric SARS-CoV-2 infectious disease assessment

Contact Info

Product

Resources

About