Immunosedimentation patterns in pathological human sera

Córdoba, Félix; González, Carlos Valdés; Rivera, Pablo

doi:10.1016/0009-8981(66)90165-3

Cited by 3 publications

(2 citation statements)

References 5 publications

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“…Since some of the coenzymes can compete successfully for the combining sites of the antibody, the effect of the purified antibodies on coenzyme-dependent enzymic activities can also be tested. Recently, antibodies specific for the coenzymes folic acid (Jaton and Ungar-Waron, 1967; Ricker and Stollar, 1967) and pyridoxal (Ungar-Waron and Sela, 1966; Cordoba et al, 1966) have been produced. Antibodies to pyridoxal did inhibit the pyridoxal-dependent enzyme, glutamic-oxalacetic transaminase (Ungar- Waron and Sela, 1966).…”

Section: Discussionmentioning

confidence: 99%

Production of antibodies with specificity toward adenosine, uridine, and ribose 5'-phosphates

Vunakis¹,

Seaman²,

Setlow³

et al. 1968

Biochemistry

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During the photooxidation of guanine-containing nucleotides and dinucleotides, the guanine moiety is destroyed to yield a reactive photointermediate which condenses with the amino groups of a copolymer composed of glutamyl and lysyl residues. Immunization of rabbits with such conjugates complexed to methylated bovine serum albumin results in the production of antibodies directed toward the haptens as well as toward the random copolymer. The specificities of the antisera to conjugates obtained with guanosine 5'-phosphate (GMP-5'), GpA, and GpU photoproducts were examined by complement fixation. The antibodies directed toward the mononucleotide coniugate show specificity toward the ribose 5 '-phosphate moieties of the nucleotides. In sera of rabbits immunized with photooxidized GpA and GpU conjugates, the antibodies are directed toward the 5 '-nucleotides, adenosine 5'-phosphate (AMP-5') and uridine 5 '-phos-

show abstract

Section: Discussionmentioning

confidence: 99%

Production of antibodies with specificity toward adenosine, uridine, and ribose 5'-phosphates

Vunakis¹,

Seaman²,

Setlow³

et al. 1968

Biochemistry

View full text Add to dashboard Cite

show abstract

“…These sera were tested by quantitative complement (C') fixation and precipitation techniques, respectively. The two sera It has recently been shown that antibodies may be produced to pyridoxal, pyridoxal 5'-phosphate, and pyridoxamine 5'-phosphate (Ungar-Waron and Sela, 1966;Cordoba et al, 1966). These antibodies were induced by immunization with antigens prepared by covalently linking the haptenic coenzymes to large carrier molecules (BSA1 or a synthetic branched polypeptide).…”

mentioning

confidence: 99%