Immunoregulatory effects of Taishan Pinus massoniana pollen polysaccharide on chicks co-infected with avian leukosis virus and Bordetella avium early in ovo

Guo, F.C.; Xue, Cong; Wu, Cun; Zhao, Xue; Qu, Tinghe; He, Xiaoyun; Guo, Zhongkun; Zhu, Rui‐Liang

doi:10.1016/j.rvsc.2013.11.010

Cited by 19 publications

(8 citation statements)

References 24 publications

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“…The immune-regulating activities of many plant polysaccharides have been studied353637. Our previous studies have proven the excellent immune enhancement effects of TPPPS on animals1638. The significant immune recovery effects of TPPPS on ALV-J-induced immunosuppression endow this polysaccharide with more prospects.…”

Section: Discussionmentioning

confidence: 99%

Taishan Pinus massoniana pollen polysaccharide inhibits subgroup J avian leucosis virus infection by directly blocking virus infection and improving immunity

Wei

Liu

et al. 2017

Sci Rep

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Subgroup J avian leucosis virus (ALV-J) generally causes neoplastic diseases, immunosuppression and subsequently increases susceptibility to secondary infection in birds. The spread of ALV-J mainly depends on congenital infection and horizontal contact. Although ALV-J infection causes enormous losses yearly in the poultry industry worldwide, effective measures to control ALV-J remain lacking. In this study, we demonstrated that Taishan Pinus massoniana pollen polysaccharide (TPPPS), a natural polysaccharide extracted from Taishan Pinus massoniana pollen, can significantly inhibit ALV-J replication in vitro by blocking viral adsorption to host cells. Electron microscopy and blocking ELISA tests revealed that TPPPS possibly blocks viral adsorption to host cells by interacting with the glycoprotein 85 protein of ALV-J. Furthermore, we artificially established a congenitally ALV-J-infected chicken model to examine the anti-viral effects of TPPPS in vivo. TPPPS significantly inhibited viral shedding and viral loads in immune organs and largely eliminated the immunosuppression caused by congenital ALV-J infection. Additionally, pre-administration of TPPPS obviously reduced the size and delayed the occurrence of tumors induced by acute oncogenic ALV-J infection. This study revealed the prominent effects and feasible mechanisms of TPPPS in inhibiting ALV-J infection, thereby providing a novel prospect to control ALV-J spread.

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Section: Discussionmentioning

confidence: 99%

Taishan Pinus massoniana pollen polysaccharide inhibits subgroup J avian leucosis virus infection by directly blocking virus infection and improving immunity

Wei

Liu

et al. 2017

Sci Rep

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“…IL-2 mainly produced by activated T lymphocytes has important immunoregulatory effects in the immune system and can enhance multiple functions of immune cells, stimulating helper T cells and natural killer cells to produce cytokines and facilitate their proliferation. Therefore, IL-2 level can indirectly reflect the level of immune response [15]. The content of cytokine IL-2 in the serum of group I was significantly higher than that of the other groups (P < 0 01), indicating that RPPS could remarkably increase the content of cytokine IL-2 in chicken serum.…”

Section: International Journal Of Polymer Sciencementioning

confidence: 89%

Immunomodulatory Effects of Robinia pseudoacacia Polysaccharides on Live Vaccine against Infectious Bronchitis in Immunosuppressive Chickens

Sun¹,

Li²,

Wang³

2019

International Journal of Polymer Science

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In order to investigate the immunomodulatory effect of Robinia pseudoacacia Polysaccharides (RPPS) on vaccine against Infectious Bronchitis (IB) in immunosuppressive chickens, the artificial leukemia chicken model was established and then the IB live vaccine (H120 strain) was immunized. The immunomodulatory efficacy of RPPS was determined by the antibody titer, the lymphocyte transformation rate in peripheral blood, the CD4+ and CD8+ T lymphocyte levels in peripheral blood, and the cytokine levels in the serum. The results showed that RPPS could not only enhance the immune effect of IB live vaccine but also improve the immunity of immunosuppressive chickens. Thus, the function of RPPS immunopotentiator could be further developed.

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“…TPPPS has been demonstrated that it can enhance the stimulating abilities of vaccines to induce better humoral and cellular immunities (Zhao et al, 2013 ; Guo et al, 2014 ). Similarly, judging from our results, TPPPS used as adjuvant exhibited a big enhancement for the immune response of the recombinant subunit.…”

Section: Discussionmentioning

confidence: 99%

Fused IgY Fc and Polysaccharide Adjuvant Enhanced the Immune Effect of the Recombinant VP2 and VP5 Subunits—A Prospect for Improvement of Infectious Bursal Disease Virus Subunit Vaccine

et al. 2017

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Infectious bursal disease virus (IBDV) is a highly contagious pathogen that causes damage in lymphoid organs and remains a threat to the poultry industry worldwide. Currently, subunit vaccines based on VP2 antigen expressed in prokaryotic systems are widely used in clinical settings. However, the immunogenicity of VP2 vaccines is limited because of their inherent defect that the structure of the antigen expressed in Escherichia coli (E. coli) may be different from its natural conformation. In this study, we fused VP2 and VP5 protective antigen genes and linked the chicken IgY Fc gene onto it. The eukaryotic expression plasmid carrying the fusion gene was transformed into Pichia pastoris (P. pastoris) to express the recombinant VP2–VP5–Fc protein. The recombinant protein was used as immunogen for evaluating immune response, and the recombinant VP2–Fc and VP2 proteins expressed in P. pastoris and the commercial VP2 subunit vaccines were used as controls. Moreover, Taishan Pinus massoniana pollen polysaccharide (TPPPS), an immunomodulator found by our laboratory, was used as adjuvant to investigate its immune modulatory effects on immunogens. Chickens were divided into six groups and inoculated with VP2–VP5–Fc+TPPPS, VP2–VP5–Fc, VP2–Fc, VP2 vaccine, commercial VP2 subunit vaccine, and phosphate buffered saline (PBS). The recombinant VP2 subunit vaccine expressed in P. pastoris exhibited higher immunogenicity than the commercial VP2 subunit vaccine. The VP2–Fc protein showed a better effect than the VP2 protein, and the VP2–VP5–Fc subunit further improved the immune effects. In addition, TPPPS was proved to be a good immunopotentiator for the VP2–VP5–Fc subunit vaccine. Hence, the recombinant VP2–VP5–Fc subunit combined with TPPPS adjuvant exhibits potential as efficient IBDV vaccine to prevent infectious bursal disease.

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Immunoregulatory effects of Taishan Pinus massoniana pollen polysaccharide on chicks co-infected with avian leukosis virus and Bordetella avium early in ovo

Cited by 19 publications

References 24 publications

Taishan Pinus massoniana pollen polysaccharide inhibits subgroup J avian leucosis virus infection by directly blocking virus infection and improving immunity

Taishan Pinus massoniana pollen polysaccharide inhibits subgroup J avian leucosis virus infection by directly blocking virus infection and improving immunity

Immunomodulatory Effects of Robinia pseudoacacia Polysaccharides on Live Vaccine against Infectious Bronchitis in Immunosuppressive Chickens

Fused IgY Fc and Polysaccharide Adjuvant Enhanced the Immune Effect of the Recombinant VP2 and VP5 Subunits—A Prospect for Improvement of Infectious Bursal Disease Virus Subunit Vaccine

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