Immunoregulatory Effects of Saengshik on DSS-Induced Inflammatory Bowel Disease in Mouse Model System

임병우,; 정용준,; 박미현,; 김종대,; 황성주,; 유병팔,

doi:10.3746/jkfn.2007.36.1.032

Cited by 1 publication

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References 24 publications

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“…It has also been shown to have higher nutritional value [ 3 ] and had a greater anti-inflammatory effect than Sunsik in our study. Several studies have reported that Saengshik has protective effects on carbon tetrachloride (CCl 4 )-induced protein oxidative damage in mouse liver, and other health benefits, such as anti-diabetes, anti-hyperlipidemia, anti-obesity, immune enhancement, anti-oxidative, detoxifying, fatty liver improving, and other effects in human and animal studies [ 4 5 6 7 8 9 10 11 ]. Natural raw meal (NRM), called raw meal in America, which is regarded as a natural health food, contains high levels of freeze-dried fresh whole grains, vegetables, and berries instead of legumes due to high allergic score [ 12 ], which is a critical difference between it and Saengshik manufactured in South Korea [ 5 ].…”

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confidence: 99%

Effects of natural raw meal (NRM) on high-fat diet and dextran sulfate sodium (DSS)-induced ulcerative colitis in C57BL/6J mice

Shin

Song

Park³

et al. 2015

Nutr Res Pract

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BACKGROUND/OBJECTIVESColitis is a serious health problem, and chronic obesity is associated with the progression of colitis. The aim of this study was to determine the effects of natural raw meal (NRM) on high-fat diet (HFD, 45%) and dextran sulfate sodium (DSS, 2% w/v)-induced colitis in C57BL/6J mice.MATERIALS/METHODSBody weight, colon length, and colon weight-to-length ratio, were measured directly. Serum levels of obesity-related biomarkers, triglyceride (TG), total cholesterol (TC), low density lipoprotein (LDL), high density lipoprotein (HDL), insulin, leptin, and adiponectin were determined using commercial kits. Serum levels of pro-inflammatory cytokines including tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, and IL-6 were detected using a commercial ELISA kit. Histological study was performed using a hematoxylin and eosin (H&E) staining assay. Colonic mRNA expressions of TNF-α, IL-1β, IL-6, inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) were determined by RT-PCR assay.RESULTSBody weight and obesity-related biomarkers (TG, TC, LDL, HDL, insulin, leptin, and adiponectin) were regulated and obesity was prevented in NRM treated mice. NRM significantly suppressed colon shortening and reduced colon weight-to-length ratio in HFD+DSS induced colitis in C57BL/6J mice (P < 0.05). Histological observations suggested that NRM reduced edema, mucosal damage, and the loss of crypts induced by HFD and DSS. In addition, NRM decreased the serum levels of pro-inflammatory cytokines, TNF-α, IL-1β, and IL-6 and inhibited the mRNA expressions of these cytokines, and iNOS and COX-2 in colon mucosa (P < 0.05).CONCLUSIONThe results suggest that NRM has an anti-inflammatory effect against HFD and DSS-induced colitis in mice, and that these effects are due to the amelioration of HFD and/or DSS-induced inflammatory reactions.

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