Immunoregulation of uveoretinal inflammation

Forrester, John V.; Lumsden, Lynne; Liversidge, Janet; Kuppner, M. C.; Mesri, Mehdi

doi:10.1016/1350-9462(94)00012-5

Cited by 23 publications

(17 citation statements)

References 109 publications

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“…Under normal circumstances, the RPE generates immunosuppressive mediators. However, after activation, as is found in the case of RPE cell rejection and as our findings imply, they may act as regulators themselves and lead to an activation of the immune system, especially of the T cells [24].…”

Section: Discussionsupporting

confidence: 74%

Influence of Interleukin 10 and Transforming Growth Factor-β on T Cell Stimulation through Allogeneic Retinal Pigment Epithelium Cells in vitro

et al. 2002

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The effect of interleukin 10 (IL-10) and transforming growth factor-β (TGF-β) on the immunological capability of human retinal pigment epithelium (RPE) cells was tested by measuring the proliferation of allogeneic T cells after stimulation through the RPE cells in vitro. For this, human RPE cells were activated with different concentrations of interferon-γ (0, 50, 100 and 250 U/ml) used as stimulator cells. The following T cell proliferation was measured with a tetrazolium-based colorimetric assay over a period of up to 4 days. Our results show that RPE cells are able to stimulate allogeneic T cells in the presence or absence of professional antigen-presenting cells (macrophages). Additionally, the proliferation could be significantly decreased by IL-10 or TGF-β. The maximum of suppression, especially by TGF-β, was found after 48 and 72 h of incubation. This shows that the antigen-presenting properties of human RPE cells can be altered in culture.

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Section: Discussionsupporting

confidence: 74%

Influence of Interleukin 10 and Transforming Growth Factor-β on T Cell Stimulation through Allogeneic Retinal Pigment Epithelium Cells in vitro

et al. 2002

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“…TGF-b is secreted in a latent form from which the mature form is released from the latency-associated peptide by proteolytic cleavage and other unknown mechanisms [9,10]. The roles of TGF-b in ocular pathophysiology and in immunoregulation are well documented [11][12][13]. Elevated expression of TGF-b in vitreous, retina and retinal pigment epithelium (RPE) has been correlated closely with retinal fibrosis and choroidal neovascularization [11].…”

Section: Introductionmentioning

confidence: 99%

Transforming growth factor-β expression in human retinal pigment epithelial cells is enhanced by Toxoplasma gondii: a possible role in the immunopathogenesis of retinochoroiditis

Nagineni

Detrick

Hooks

2002

Clinical and Experimental Immunology

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SUMMARYRetinochoroiditis caused by Toxoplasma gondii infection results in inflammation and necrosis of the retina. We have used human retinal pigment epithelial cultures (HRPE) as an in vitro model to investigate the role of TGF-b in T. gondii-induced retinochoroiditis. RT-PCR analyses showed enhanced steady state levels of TGF-b1 and TGF-b2 mRNA in T. gondii-infected HRPE. Uninfected HRPE secrete TGF-b1 in a latent form while 10-30% of the secreted TGF-b2 was in the active form. T. gondii infection induced a significant increase (P < 0·01) in total TGF-b1 and TGF-b2 secretion by HRPE. In addition, soluble extracts of T. gondii (ST) stimulated secretion of both TGF-b1 and TGF-b2 significantly (P < 0·01). Interestingly, T. gondii infection as well as ST of the parasites completely inhibited secretion of the active form of TGF-b2. Studies evaluating the effect of TGF-b on T. gondii replication in HRPE revealed that TGF-b enhanced parasite replication. The interactions between host retinal cells and T. gondii may play an active role in the pathogenesis of retinochoroiditis.

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“…However, CRFB4 was detectable and has been recently identified as a signal-transducing chain of the IL-10 receptor [32]. Results have demonstrated that the CRFB4 chain is part of the IL-10 receptor signalling complex and serves as an accessory IL-10R β-chain, essential for an active receptor complex [16].…”

Section: Discussionmentioning

confidence: 99%

The influence of pro-inflammatory cytokines on human retinal pigment epithelium cell receptors

Hollborn

Kohen

Wiedemann

et al. 2001

Graefe's Arch Clin Exp Ophthalmol

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Our results demonstrate that human RPE cells express the mRNA of different cytokine receptors and the expression may be partially modulated by pro-inflammatory cytokines. This may show that RPE cells act as corresponding cells not only in vitro, but also in inflammation and immunological processes in the eye. In this connection it could be hypothesised that activated RPE cells play a stimulating role in addition to the known suppressive one.

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Immunoregulation of uveoretinal inflammation

Cited by 23 publications

References 109 publications

Influence of Interleukin 10 and Transforming Growth Factor-β on T Cell Stimulation through Allogeneic Retinal Pigment Epithelium Cells in vitro

Influence of Interleukin 10 and Transforming Growth Factor-β on T Cell Stimulation through Allogeneic Retinal Pigment Epithelium Cells in vitro

Transforming growth factor-β expression in human retinal pigment epithelial cells is enhanced by Toxoplasma gondii: a possible role in the immunopathogenesis of retinochoroiditis

The influence of pro-inflammatory cytokines on human retinal pigment epithelium cell receptors

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