Immunoreactivity pattern of calretinin in the developing human cerebellar cortex

Pibiri, Valeria; Gerosa, Clara; Vinci, Laura; Faa, Gavino; Ambu, Rossano

doi:10.1016/j.acthis.2017.01.005

Cited by 8 publications

(5 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since the 1980s, with the advent of immunohistochemical strategies for identifying cell types, researcher had begun correlating cell-specific neurochemical marker expression (typically Ca 2+ -binding proteins, neuropeptides and certain receptors) with morphological and electrophysiological characterization in order to distinguish several subtypes of GABAergic interneurons (Kubota et al, 1993, 2011; Kubota and Kawaguchi, 1994, 1997; Cauli et al, 2014). Recent advances suggest that neurons expressing an unidentified cytoplasmic antigen Rat-303 (Hockfield, 1987; Hockberger et al, 1994; Geurts et al, 2001), the metabotropic glutamate and serotonin receptors (Neki et al, 1996; Geurts et al, 2001, 2002; Simat et al, 2007; Sillitoe et al, 2008), the neuropeptide somatostatin (SOM; Johansson et al, 1984; Geurts et al, 2001; Galas et al, 2017) and Ca 2+ -binding proteins such as parvalbumin and calretinin, respectively (PRV and CRT; Schneeberger et al, 1985; Rogers, 1989; Geurts et al, 2001; Schwaller et al, 2002; Bastianelli, 2003; Pibiri et al, 2017), account for nearly 100% of cerebellar GABAergic interneurons. Rat-303 antibody selectively stains Golgi cells and LCs (Hockfield, 1987; Rogers, 1989; Dieudonné and Dumoulin, 2000; Geurts et al, 2001, 2002).…”

Section: Cerebellar Interneurons Classificationmentioning

confidence: 99%

Diverse Neuron Properties and Complex Network Dynamics in the Cerebellar Cortical Inhibitory Circuit

Prestori

Mapelli

D’Angelo

2019

Front. Mol. Neurosci.

View full text Add to dashboard Cite

Neuronal inhibition can be defined as a spatiotemporal restriction or suppression of local microcircuit activity. The importance of inhibition relies in its fundamental role in shaping signal processing in single neurons and neuronal circuits. In this context, the activity of inhibitory interneurons proved the key to endow networks with complex computational and dynamic properties. In the last 50 years, the prevailing view on the functional role of cerebellar cortical inhibitory circuits was that excitatory and inhibitory inputs sum spatially and temporally in order to determine the motor output through Purkinje cells (PCs). Consequently, cerebellar inhibition has traditionally been conceived in terms of restricting or blocking excitation. This assumption has been challenged, in particular in the cerebellar cortex where all neurons except granule cells (and unipolar brush cells in specific lobules) are inhibitory and fire spontaneously at high rates. Recently, a combination of electrophysiological recordings in vitro and in vivo, imaging, optogenetics and computational modeling, has revealed that inhibitory interneurons play a much more complex role in regulating cerebellar microcircuit functions: inhibition shapes neuronal response dynamics in the whole circuit and eventually regulate the PC output. This review elaborates current knowledge on cerebellar inhibitory interneurons [Golgi cells, Lugaro cells (LCs), basket cells (BCs) and stellate cells (SCs)], starting from their ontogenesis and moving up to their morphological, physiological and plastic properties, and integrates this knowledge with that on the more renown granule cells and PCs. We will focus on the circuit loops in which these interneurons are involved and on the way they generate feed-forward, feedback and lateral inhibition along with complex spatio-temporal response dynamics. In this perspective, inhibitory interneurons emerge as the real controllers of cerebellar functioning.

show abstract

Section: Cerebellar Interneurons Classificationmentioning

confidence: 99%

Diverse Neuron Properties and Complex Network Dynamics in the Cerebellar Cortical Inhibitory Circuit

Prestori

Mapelli

D’Angelo

2019

Front. Mol. Neurosci.

View full text Add to dashboard Cite

show abstract

“…Interestingly there is another type of calcium buffer that is extensively expressed in the granule cells and unipolar brush cells but is typically absent from adult Purkinje cells, calretinin (Dino et al 1999). However, a recent study shows that calretinin expression is transiently observed in Purkinje cells and molecular layer interneurons during human embryonic development (Pibiri et al 2017) development in Purkinje cells (Fig. 2), although a similar developmental transition has not been identified to date in rodent models to our knowledge.…”

Section: Calcium Buffersmentioning

confidence: 82%

Development of Physiological Activity in the Cerebellum

Jayabal

Watt

2021

Handbook of the Cerebellum and Cerebellar Disorders

View full text Add to dashboard Cite

One remarkable aspect of cerebellar development is that intrinsic physiological activity of several neuronal cell types, including Purkinje cells, can be observed throughout a large portion of the developmental window. Although ion channels primarily drive this intrinsic activity, it can also be influenced by other cellular properties and inputs, including synaptic and neuromodulatory inputs, calcium buffers, and others. Many of the factors that drive or influence intrinsic activity are expressed in a tightly regulated manner during the development of the cerebellum. Here, we review how the ion channels, calcium buffers, synapses, and neuromodulators that are differentially expressed during development give

show abstract

“…In lamprey's SC different types of neurons expressed different intensity of calretinin, sometimes in opposite ways (Viloria et al, 2008). These could point to role of calretinin as regulator of neuronal excitability (Viloria et al, 2008; Pibiri et al, 2017). Integrated nociceptive informations formed in the spinal dorsal horn are important for finding targets for pain therapy (Cordero‐Erausquin et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

“…In the chicken embryonal SGs, calretinin was noticed only after establishing periphery target connection (Király et al, 1993). In developing human cerebellum at 11 weeks no calretinin was found while in week 18 th Purkinje cells and migrating cerebellar interneurons were labelled (Pibiri et al, 2017). In lamprey's SC different types of neurons expressed different intensity of calretinin, sometimes in opposite ways (Viloria et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…It was never found in the motoneurons (Résibois and Rogers, 1992). Various neuronal subpopulations in zebrafish (Castro et al, 2006), lamprey's SC (Viloria et al, 2008), Purkinje cells and migrating cerebellar interneurons in human (Yew et al, 1997; Pibiri et al, 2017) expressed calretinin. Since we have long time experience in the investigation of the neurulation mechanisms in human development, especially those of spinal cord (Saraga‐Babić et al, 1995; Saraga Babic et al, 1996; Saraga‐Babic et al, 2002; Vukojevic et al, 2016) in the present study we tried to elucidate the spatio‐temporal distribution of several neuronal markers in the different zones of the human SC at early embryonic stages.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Time course and expression pattern of the neuronal markers in the developing human spinal cord

Restović

Bočina

Vukojević

et al. 2019

Intl J of Devlp Neuroscience

View full text Add to dashboard Cite

The aim of this study was to examine the spatio‐temporal appearance of different neuronal cell subtypes by analyzing expression patterns of several neuronal markers (calretinin, neurofilament 200 (NF200), vanilloid receptor 1(VR1) and calcitonin gene‐related peptide (CGRP)) of the embryonic human spinal cord (SC). Developing human SCs from 11 human conceptuses beetwen 5–10 developmental weeks (DW) were examined by light and electron microscopy and immunofluorescence. Light and electron microscopy revealed different embryonic stages of recognizable structure of the SC. NF200, CGRP and VR1 positive cells were observed in SCs during 5th–6th DW. NF200 was predominantly expressed in the ventral part, indicating presence of motoneurons. As development advanced, NF200 was mainly expressed in the marginal zone. Expression of CGRP was intense during all of the investigated periods, predominantly during the 5th–6th DW pointing to neural sensory differentiation, as opposed to the last DW when reduced expression of CGRP in the marginal layer indicated the terminations of the sensory afferents. Expression of VR1 was highest in the intermediate zone, at the beginning and at the end of the investigated periods, pointing to VR1 spatial pattern in the visceral afferents in the grey matter, while the first signs of calretinin were found in the 9th–10th DW ventrally. Delineating the relationships between factors involved in processes of neuronal differentiation as well as spatial and temporal arrangement of SC interrelated neurons can provide a useful information about normal SC development as well as the insight in possible causes of anomalies and disorders during embryonic life.

show abstract

Immunoreactivity pattern of calretinin in the developing human cerebellar cortex

Cited by 8 publications

References 27 publications

Diverse Neuron Properties and Complex Network Dynamics in the Cerebellar Cortical Inhibitory Circuit

Diverse Neuron Properties and Complex Network Dynamics in the Cerebellar Cortical Inhibitory Circuit

Development of Physiological Activity in the Cerebellum

Time course and expression pattern of the neuronal markers in the developing human spinal cord

Contact Info

Product

Resources

About