Immunoreactivity of progesterone receptor isoform B, nuclear factor κB, and IκBα in adenomyosis

Nie, Jichan; Lü, Yuan; Liu, Xishi; Guo, Sun‐Wei

doi:10.1016/j.fertnstert.2009.01.084

Cited by 68 publications

(72 citation statements)

References 35 publications

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“…That immunoreactivity to PR-B is reduced, while NF-kB p65, COX-2, OTR, and TRPV1 immunostaining levels are all elevated in ectopic endometrium in mice with adenomyosis as compared with those without is consistent with human studies, so did their correlation with the severity of dysmenorrhea. [45][46][47][48] In particular, elevated myometrial OTR expression is consistent with our recent report that myometrial OTR expression is increased in women with adenomyosis and is positively correlated with the uterine contractility and the severity of dysmenorrhea. 8 The finding that is somewhat novel arising from this study is the increased staining for collagen I and IV, even though our results are consistent with the finding of increased interstitial collagen deposition in CD-1 mice with adenomyosis that was induced exactly the same manner as in our study.…”

Section: Discussionsupporting

confidence: 91%

Possible Loss of GABAergic Inhibition in Mice With Induced Adenomyosis and Treatment With Epigallocatechin-3-Gallate Attenuates the Loss With Improved Hyperalgesia

et al. 2014

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We have previously reported that induction of adenomyosis in mice results in progressive hyperalgesia, uterine hyperactivity, and elevated plasma corticosterone levels and that epigallocatechin-3-gallate (EGCG) treatment dose dependently suppressed myometrial infiltration and improved generalized hyperalgesia. In this study, we examined whether adenomyosis induced in mice results in the loss of GABAergic inhibition as manifested by the diminished glutamate decarboxylase (GAD) 65-expressing neurons in the brainstem nucleus raphe magnus (NRM) that could correlate with heightened hyperalgesia. We also evaluated whether EGCG treatment would reverse these changes and also improve the expression of some proteins known to be involved in adenomyosis. Adenomyosis was induced in 28 female ICR mice and additional 12 were used as blank controls, as reported previously. At the 16th week, all mice with induced adenomyosis received low-or high-dose EGCG treatment or untreated. Mice without adenomyosis received no treatment. After 3 weeks of treatment, their uterine horns and brains were harvested. The right uterine horn was used for immunohistochemistry analysis and for counting the number of macrophages infiltrating into the ectopic endometrium. The brainstem NRM sections were subjected to immunofluorescence staining for GAD65. We found that mice with induced adenomyosis had significantly diminished GAD65-expressing neurons, concomitant with heightened hyperalgesia. Treatment with EGCG increased these neurons in conjunction with improved hyperalgesia, reduced the expression of pp65, cycloxygenase 2, oxytocin receptor, collagen I and IV, and transient receptor potential vanilloid type 1 in ectopic endometrium or myometrium, reduced the number of macrophages infiltrating into the ectopic endometrium while elevated the expression of progesterone receptor isoform B. Thus, adenomyosis-induced pain resembles neuropathic pain in that there is a remarkable central plasticity.

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Section: Discussionsupporting

confidence: 91%

Possible Loss of GABAergic Inhibition in Mice With Induced Adenomyosis and Treatment With Epigallocatechin-3-Gallate Attenuates the Loss With Improved Hyperalgesia

et al. 2014

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“…The patients recruited to this study were reported previously [19,21]. Briefly, 50 women with histologically confirmed adenomyosis (excluding endometriosis) seen at Shanghai Obstetrics and Gynecology Hospital, Fudan University, were recruited for this study.…”

Section: Methodsmentioning

confidence: 99%

“…In women with adenomyosis and without, exactly half of them were in the secretory phase. Their amount of menses during menstruation was grouped into three classes: light, moderate, and heavy, and the severity of dysmenorrhea was classified as mild, moderate, and severe, as reported previously [19,21]. …”

Section: Methodsmentioning

confidence: 99%

“…For each patient with adenomyosis, information was collected through reading medical charts and interviewing patients on age at surgery, uterus size (estimated volume []see [19,21]), complaint of dysmenorrhea, severity of dysmenorrhea, duration of dysmenorrhea, amount of menses, and gravidity. This study was approved by the institutional ethics review board of Shanghai Obstetrics and Gynecology Hospital.…”

Section: Methodsmentioning

confidence: 99%

“…We also correlated their immunoreactivity levels with the patients’ symptomology such as dysmenorrhea status and/or severity, amount of menses, and uterus size. Finally, we evaluated correlation coefficients of the DNMT immunoreactivity levels with other proteins that we have reported previously, including NF-ĸB [19], SLIT [20], oxytocin receptor (OTR) and transient receptor potential vanilloid type 1 (TRPV1) [21]. This may provide some much needed clues suggesting that some of these aberrations may be epigenetically controlled.…”

Section: Introductionmentioning

confidence: 99%

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Aberrant Immunoreactivity of Deoxyribonucleic Acid Methyltransferases in Adenomyosis

Liu

Guo²

2012

Gynecol Obstet Invest

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Objectives: To investigate the expression and localization of deoxyribonucleic acid methyltransferases (DNMTs) in women with and without adenomyosis. Study Design: Ectopic and homologous eutopic endometrium from 50 women with adenomyosis and endometrium from 18 age- and menstrual phase-matched women without adenomyosis were used for immunohistochemical analysis. Tissue sections were immunostained with DNMT1, DNMT3A, and DNMT3B. Microscopic evaluation to assess the presence and localization of DNMT1, DNMT3A, and DNMT3B throughout the menstrual cycle in both eutopic endometrial and endometriotic tissues of women with adenomyosis was performed, and comparison with normal endometrium was made. Results: Compared with normal endometrium, immunoreactivity to DNMT1 and DNMT3B was higher in ectopic endometrium, while DNMT3A staining levels were significantly reduced in both eutopic and ectopic endometrium. DNMT1 immunoreactivity in eutopic endometrium was positively associated with heavier menses. Increased DNMT3B immunoreactivity in ectopic, but not eutopic, endometrium was associated with the severity of dysmenorrhea. Conclusions: The immunoreactivity to DNMTs in adenomyosis differs significantly from that in normal endometrium, suggesting that adenomyosis may be an epigenetic disease. The finding that DNMT3B correlates with the severity of dysmenorrhea suggests that DNMTs may be involved in adenomyosis-caused dysmenorrhea and its severity. Thus, therapeutics based on epigenetic rectification may hold promise in the management of adenomyosis.

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The Endometrium in Adenomyosis

Habiba¹,

Benagiano²

2015

Uterine Adenomyosis

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Immunoreactivity of progesterone receptor isoform B, nuclear factor κB, and IκBα in adenomyosis

Cited by 68 publications

References 35 publications

Possible Loss of GABAergic Inhibition in Mice With Induced Adenomyosis and Treatment With Epigallocatechin-3-Gallate Attenuates the Loss With Improved Hyperalgesia

Possible Loss of GABAergic Inhibition in Mice With Induced Adenomyosis and Treatment With Epigallocatechin-3-Gallate Attenuates the Loss With Improved Hyperalgesia

Aberrant Immunoreactivity of Deoxyribonucleic Acid Methyltransferases in Adenomyosis

The Endometrium in Adenomyosis

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