Immunoreactivity of MAPK Signaling in a Rat Model of Intrauterine Growth Retardation Induced by Uterine Artery Ligation

Choi, Myeon-Song; Chung, Yoon Young; Kim, Dong Joon; Kim, Seong Taeck; Jun, Yong Hyun

doi:10.21873/invivo.11819

Cited by 6 publications

(5 citation statements)

References 53 publications

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“…In another transient cerebral ischemia/reperfusion model, DEX induced neuronal survival in the hippocampus and cortex by up-regulating the PI3K/AKT and ERK pathways (38). In our previous studies, both AKT and ERK expression decreased with BDNF down-regulation under prenatal hypoxic conditions (20,39). We propose that DEX treatment induces high BDNF expression in BCCAO.…”

Section: Figure 3 Representative Photograph and Proportion Of Neun-po...mentioning

confidence: 62%

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Dexmedetomidine Stereotaxic Injection Alleviates Neuronal Loss Following Bilateral Common Carotid Artery OcclusionviaUp-Regulation of BDNF Expression

KIM,

SHIN,

CHO

et al. 2023

In Vivo

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Background/Aim: Neurogenesis is an important process in the recovery from neurological damage caused by ischemic lesions. Endogenous neurogenesis is insufficient to restore neuronal damage following cerebral ischemia. Dexmedetomidine (DEX) exerts neuroprotective effects against cerebral ischemia and ischemia/reperfusion injury. DEX promotes neurogenesis, including neuronal proliferation and maturation in the hippocampus. In a previous study, we showed that early neurogenesis increased 3 days after bilateral common carotid artery occlusion (BCCAO). In this study, we investigated the effect of DEX on neurogenesis 3 days after BCCAO. Materials and Methods: Male Sprague-Dawley (SD) rats (7-8 weeks old) were used as a BCCAO model. Right and left common carotid arteries of the rats were occluded using 4-0 silk sutures. Two hours after surgery, an intracranial DEX injection was administered to rats that underwent surgery using a stereotaxic injector. Brains were obtained from control and BCCAO rats 3 days after surgery. Immunohistochemistry was performed on the cortex and dentate gyrus of the hippocampus using a NeuN antibody. Western blot was performed with HIF1α and brain-derived neurotrophic factor (BDNF) antibodies. Results: The number of mature neurons decreased 3 days after BCCAO, but DEX treatment alleviated neural loss in the parietal cortex and hippocampus. Up-regulation of BDNF was also observed after dexmedetomidine treatment. Conclusion: Stereotaxic injection of dexmedetomidine alleviates neural loss following BCCAO by up-regulating BDNF expression. Materials and MethodsAnimal surgery. Male Sprague-Dawley (SD) rats (7-8 weeks old) were supplied by a certified breeder (Damul Laboratory Animals, Daejeon, Republic of Korea). All animal experiments were 184

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Section: Figure 3 Representative Photograph and Proportion Of Neun-po...mentioning

confidence: 62%

“…Brains were fixed with a 4% paraformaldehyde solution for 3 days at 4˚C. Paraffin sections were prepared using fixed brains as described previously (20). The cerebrums were separated from the brain stem and were cut at sagittal plane intervals of 7-8 μm.…”

mentioning

confidence: 99%

Dexmedetomidine Stereotaxic Injection Alleviates Neuronal Loss Following Bilateral Common Carotid Artery OcclusionviaUp-Regulation of BDNF Expression

KIM,

SHIN,

CHO

et al. 2023

In Vivo

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“…In addition, miR-3175 participates in the MAPK-related signaling pathway, whereas MAP3K9 is an upstream activator of MAPK signaling [35,36]. Choi et al also found the immunoreactivity of MAPK signaling in FGR rats [37]. Therefore, miR-3175-MAP3K9 may trigger the pathogenesis of FGR by regulating the immune activity of the downstream MAPK pathway.…”

Section: Discussionmentioning

confidence: 99%

Integrative analysis of the immune-related ceRNA network in fetal growth restriction based on weighted gene co-expression network analysis

Zhao

2022

Arch Gynecol Obstet

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Immune disorders lead to placental dysfunction and fetal growth restriction (FGR), but current research on the immune regulation mechanisms of FGR is insufficient. Therefore, this study aimed to construct an immune-related competing endogenous RNA (ceRNA) network to predict FGR onset risk. Methods: Based on microarray data from the GEO database, CIBERSORT was used to analyze immune cell infiltration. Weighted gene co-expression network analysis (WGCNA) was used to screen immune-related module genes with differential expression (DE) in FGR. A ceRNA network was constructed by integrating long non-coding RNA (lncRNA)-mRNA co-expression relations, lncRNA-microRNA (miRNA) relations, and miRNA-mRNA negative regulatory relations. The diagnostic values of key genes in the network and their relationships with immune cell infiltration were 2 further validated. Results: By comparing FGR and normal samples, 442 DE mRNAs, 57 DE miRNAs, and 241 DE lncRNAs were obtained. Furthermore, four types of immune cells exhibited significantly different infiltration levels. WGCNA revealed 236 immune-related DE mRNAs that were involved in hormone secretion and immune cell differentiation. We then constructed a ceRNA network comprising 16 lncRNAs, 16 miRNAs, and 21 mRNAs through co-expression analysis and miRNA prediction.Receiver operating characteristic curves confirmed the superior predictive performance of key genes in the network for FGR. The validation dataset confirmed the increased infiltration of M1 macrophages in FGR samples and their positive correlations with NEURL1 and ODF3B expression. Conclusion:We constructed an immune-related ceRNA regulatory network wherein key genes are positively correlated with M1 macrophage infiltration and can be used for FGR diagnosis.

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“…The highly expressed tsRNAs were mainly involved in the biological process of embryonic development and cell connection, and most significantly enriched pathways were GnRH signaling pathway, insulin signaling pathway, MAPK signaling pathway, melanogenesis, and focal adhesion signaling pathways. The GnRH signaling pathway ( Korpysz and Szalecki, 2019 ), insulin signaling pathway ( Dunlop et al, 2021 ), and MAPK signaling pathway ( Choi et al, 2020 ) have been reported to be associated with IUGR. In addition, differentially expressed tsRNAs in normal-size and IUGR porcine skeletal muscle were mainly enriched in metabolic pathways, Rap1 signaling pathway, endocytosis, mTOR signaling pathway, and AMPK signaling pathway.…”

Section: Discussionmentioning

confidence: 99%

Identification of tRNA-derived small RNAs and their potential roles in porcine skeletal muscle with intrauterine growth restriction

Gan

Chen

et al. 2022

Front. Physiol.

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Intrauterine growth restriction (IUGR) in humans often manifests as poor growth and delayed intellectual development, whereas in domestic animals it results in increased mortality. As a novel epigenetic regulatory molecule, tRNA-derived small RNAs (tsRNAs) have been reported to be involved in many biological processes. In this study, pigs (35d) were used as a model to characterize tsRNAs by sequencing in normal and IUGR porcine skeletal muscle. A total of 586 tsRNAs were identified, of which 103 were specifically expressed in normal-size pigs and 38 were specifically expressed in IUGR pigs. The tsRNAs formed by splicing before the 5′ end anti codon of mature tRNA (tRF-5c) accounted for over 90% of tsRNAs, which were significantly enriched in IUGR pigs than in normal-size pigs. Enriched pathways of differentially expressed tsRNAs target genes mainly included metabolic pathways, Rap1 signaling pathway, endocytosis, mTOR signaling pathway, and AMPK signaling pathway. Regulatory network analysis of target genes revealed that IGF1 was one of the most important molecules of regulatory nodes in IUGR and normal porcine skeletal muscle. In addition, IGF1 was found to be one of the target genes of tRF-Glu-TTC-047, which is a highly expressed tsRNA in IUGR pigs. The findings described herein uncover the role of tsRNAs in IUGR porcine skeletal muscle development, thus providing insights into the prevention and treatment of IUGR in mammals.

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Immunoreactivity of MAPK Signaling in a Rat Model of Intrauterine Growth Retardation Induced by Uterine Artery Ligation

Cited by 6 publications

References 53 publications

Dexmedetomidine Stereotaxic Injection Alleviates Neuronal Loss Following Bilateral Common Carotid Artery OcclusionviaUp-Regulation of BDNF Expression

Dexmedetomidine Stereotaxic Injection Alleviates Neuronal Loss Following Bilateral Common Carotid Artery OcclusionviaUp-Regulation of BDNF Expression

Integrative analysis of the immune-related ceRNA network in fetal growth restriction based on weighted gene co-expression network analysis

Identification of tRNA-derived small RNAs and their potential roles in porcine skeletal muscle with intrauterine growth restriction

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