Immunoreactivities of IL-1β and IL-1R in oviduct of Chinese brown frog ( Rana dybowskii ) during pre-hibernation and the breeding period

Hu, Ruiqi; Liu, Yuning; Deng, Yu; Ma, Sihui; Sheng, Xia; Wang, Qiang; Xu, Meiyu

doi:10.1016/j.acthis.2015.12.006

Cited by 7 publications

(7 citation statements)

References 36 publications

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“…In summary, our previous studies showed that IL-1Î and IL-1R were found in the oviducts of Rana dybowskii, and IL-1Î might be involved in immune and inflammatory responses. 18 The present study demonstrated that TLR4, MyD88, IRAK4 and NF-ÎB were expressed in the oviducts of Rana dybowskii during the breeding period and pre-hibernation. These results suggested that TLR4 might regulate the secretion of cytokines via the MyD88 signaling pathway, which plays a regulatory role in the immune response of Rana dybowskii oviducts.…”

Section: Discussionmentioning

confidence: 48%

“…The epithelial cells were divided into epithelial cells without cilia in the pre-hibernation and epithelial cells with cilia in the breeding period, which was in accordance with previous studies. 18 Cilia were observed predominantly in the epithelial cells during the breeding period, but not in the pre-hibernation (Figure 2 c,e). Glandular cells were obviously expanded in the pre-hibernation compared to the breeding period, while the epithelial cells were not observed enlargement (Figure 2 d,f).…”

Section: Histologymentioning

confidence: 98%

“…17 Previous study has shown that IL-1β and IL1R1 proteins have significant higher expressions in the oviduct of Rana dybowskii during pre-hibernation than those of the breeding period. 18 To extend our understanding of the regulation of oviductal functions in Rana dybowskii, we investigated localization and expression levels of TLR4/MyD88 signal pathway in Rana dybowskii oviducts during the breeding period and pre-hibernation, aiming to clarify the relationship between the TLR4/MyD88 signaling pathway and the oviductal functions in Rana dybowskii. Frog Breeding Farm, Jilin province, China.…”

Section: Introductionmentioning

confidence: 99%

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Expressions of TLR4, MyD88, IRAK4 and NF-κΒ in the oviduct of Chinese brown frog (Rana dybowskii)

Zhang

Zhao

et al. 2019

Eur J Histochem

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One special physiological phenomenon of the Chinese brown frog (Rana dybowskii) is that its oviduct goes through expansion prior to pre-hibernation instead of expanding during the breeding period. Our previous study discovered that some cytokines such as interleukin-1 beta (IL-1β) and its receptor type I (IL1R1) proteins were expressed in the oviduct of Rana dybowskii. In this study, we continued to investigate the localizations and expression levels of cytokines including toll-like receptor 4 (TLR4) and its related factors, myeloid differentiation factor 88 (MyD88), interleukin-1 receptor-associated kinase (IRAK) and nuclear factor kappa B (NF-κΒ) in the oviduct of Rana dybowskii during the breeding period and pre-hibernation. Morphological results showed that there were significant differences in oviductal weight and pipe diameter with significantly higher values in pre-hibernation than those of the breeding period. Strong immunostaining of TLR4, MyD88, IRAK4 and NF-κΒ were observed in the pre-hibernation. These proteins levels had no significant difference between these two periods except MyD88 which was significantly higher in the pre-hibernation. The mRNA expression levels of MyD88 and NF-κΒ were obviously higher in pre-hibernation than those of the breeding period. These findings suggested that TLR4/MyD88 signal pathway might participate in the oviductal functions of Rana dybowskii during the breeding period and pre-hibernation.

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Section: Discussionmentioning

confidence: 48%

Section: Histologymentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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Expressions of TLR4, MyD88, IRAK4 and NF-κΒ in the oviduct of Chinese brown frog (Rana dybowskii)

Zhang

Zhao

et al. 2019

Eur J Histochem

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“…These research focus on feeding and satiety signals, seasonal timing mechanisms, fuel utilization, immune function, stress responses, and adaptive mechanisms against muscle disuse atrophy. In amphibians and reptiles like R. sylvatica (Storey & Storey, 2017), Rana dybowskii (Hu et al, 2016), Polypedates megacephalus (Weng et al, 2016), Graptemys geographica (Jackson & Ultsch, 2010), P. vitticeps (Capraro et al, 2019), and Alligator sinensis (Tang et al, 2019), studies of hibernation include cold tolerance, frost tolerance, hypoxia tolerance, immunity, energy distribution, and the seasonal adaptation of intestinal flora.…”

Section: Introductionmentioning

confidence: 99%

Molecular mechanism of Chinese alligator (Alligator sinensis) adapting to hibernation

et al. 2020

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Hibernation is a physiological state for Chinese alligators to cope with cold weather. In mammals, gene expression changes during hibernation and their regulatory mechanisms have been extensively studied, however, these studies in reptiles are still rare. Here, integrated analysis of messenger RNA (mRNA), microRNA (miRNA), and long noncoding RNA (lncRNA) reveals the molecular mechanisms of the hypothalamus, liver, and skeletal muscle in hibernating and active individuals. During hibernation, the number of genes increased in the hypothalamus, liver, and skeletal muscle was 585, 282, and 297, while the number of genes decreased was 215, 561, and 627, respectively, as compared with active individuals. Through Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis, the differential expressed genes were mainly enriched in DNA damage repair, biological rhythm, energy metabolism, myoprotein degradation, and other related items and pathways. Besides, 4740 miRNAs were identified in three tissues. Through the comprehensive analysis of miRNA and mRNA abundance profiles, 12,291, 6997, and 8232 miRNA–mRNA pairs all showed a negative correlation in the hypothalamus, liver, and skeletal muscle, respectively. Some miRNA target genes were related tobiological rhythm and energy metabolism, suggesting that miRNA may play an important role in the physiological metabolism of the hibernating adaptability of Chinese alligators. Moreover, 402, 230, and 130 differentially expressed lncRNAs were identified in the hypothalamus, liver, and skeletal muscle, respectively. The targeting relationship of four lncRNA–mRNA pairs were predicted, with the main function of target genes involved in the amino acid transportation. These results are helpful to further understand the molecular regulatory basis of the hibernation adaptation in Chinese alligators.

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“…Unexpectedly, the oviduct of R. dybowskii usually expands during the period of prehibernation, instead of the breeding period (Figure 2). Meanwhile, levels of some immunomodulatory cytokines and receptor proteins, including interleukin- (IL-) 1 β , interleukin-1 receptor type 1 (IL1R1), peroxisome proliferator-activated receptor- (PPAR-) γ 2, leptin, and leptin receptor, are also higher in the oviduct during prehibernation compared with the breeding period [20, 21]. Thus, it is the optimum moment to harvest OR from R. dybowskii in autumn.…”

Section: Introductionmentioning

confidence: 99%

Traditional Uses, Bioactive Constituents, Biological Functions, and Safety Properties of Oviductus ranae as Functional Foods in China

Zhang

Wang

et al. 2019

Oxidative Medicine and Cellular Longevity

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Oviductus ranae is an animal-based traditional Chinese material widely used as tonics in China for hundreds of years. Various bioactive components are present in OR including proteins, amino acids, steroids, fatty acids, phospholipids, nucleosides, vitamins, hydantoins, and mineral elements. These constituents exert a myriad of biological functions such as immunomodulatory, antioxidant, antifatigue, antiaging, estrogen-like, hepatoprotective, hypolipidemic, antiosteoporotic, antidepressant, antitumor, antitussive, expectorant, anti-inflammatory, and antiasthmatic activities. Unlike other traditional Chinese crude drugs recorded in Chinese Pharmacopoeia, OR is seldom prescribed as medicine but often consumed as nutraceuticals to optimize health. In this review, the traditional uses, bioactive constituents, biological functions, and safety properties of OR as functional foods in China were summarized and discussed. It is expected that this review will provide useful information for anyone who is interested in OR.

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Immunoreactivities of IL-1β and IL-1R in oviduct of Chinese brown frog ( Rana dybowskii ) during pre-hibernation and the breeding period

Cited by 7 publications

References 36 publications

Expressions of TLR4, MyD88, IRAK4 and NF-κΒ in the oviduct of Chinese brown frog (Rana dybowskii)

Expressions of TLR4, MyD88, IRAK4 and NF-κΒ in the oviduct of Chinese brown frog (Rana dybowskii)

Molecular mechanism of Chinese alligator (Alligator sinensis) adapting to hibernation

Traditional Uses, Bioactive Constituents, Biological Functions, and Safety Properties of Oviductus ranae as Functional Foods in China

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