Immunoreactive β-endorphin in sheep ovary

Lim, Alan T.; Lolait, Stephen J.; Barlow, John W.; O, Wai Sum; Zois, I; Toh, Ban Hock; Funder, John W.

doi:10.1038/303709a0

Cited by 107 publications

(43 citation statements)

References 16 publications

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“…Characteristics and specificity of the ,B-EP and Nac-EP radioimmunoassays have been previously described in detail (10)(11)(12)(13). The primary antibody (R56) employed in ,B-EP radioimmunoassay studies was raised against synthetic ovine 63-EPI,3, (Peninsula Laboratories, Inc., Belmont, CA) and recognizes the undecapeptide sequence f3-EP,7-27.…”

Section: Methodsmentioning

confidence: 99%

Pro-opiomelanocortin messenger ribonucleic acid and posttranslational processing of beta endorphin in spleen macrophages.

Lolait¹,

Clements²,

Markwick³

et al. 1986

J. Clin. Invest.

210

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Section: Methodsmentioning

confidence: 99%

Pro-opiomelanocortin messenger ribonucleic acid and posttranslational processing of beta endorphin in spleen macrophages.

Lolait¹,

Clements²,

Markwick³

et al. 1986

J. Clin. Invest.

210

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“…β-Endorphin, one of the well-known representatives of this group of factors, has been found in the corpora lutea (CL) of sheep [31] and cows [10], in granulosa and interstitial cells of rodents [34,35], and in human ovary [3]. Met-enkephalin, another extensively examined opioid peptide, has been demonstrated in bovine, rabbit and human ovaries [6,30,41].…”

Section: Introductionmentioning

confidence: 99%

The physiological role of ?-endorphin in porcine ovarian follicles

et al. 2000

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-β-Endorphin-like immunoreactivity (β-END-LI) was measured by radioimmunoassay in porcine ovarian follicular fluid (FF) from small, medium and large follicles throughout the oestrous cycle. The concentration of β-END-LI in FF from small follicles collected on days 1-5 of the cycle was at least tenfold higher than in the fluid from any other follicles independently from their size and the period of the cycle. The level of β-END-LI in small follicles on days 6-10 was drastically decreased. Subsequently, on days 11-16 its concentration was enhanced and reduced again in preovulatory period of the cycle. Concentrations of β-END-LI in FF from medium follicles were relatively equal throughout the cycle (days 6-21). No significant differences in β-END-LI levels were found between small, medium and large follicles from days 17-21. However, β-END-LI concentrations in medium follicles on days 11-13 and 14-16 were statistically lower than those in small follicles. Moreover, the effects of FSH, prolactin (PRL), progesterone (P 4 ), testosterone (T) and 17 β-oestradiol (E 2 ) on β-END-LI release by granulosa cells (GCs) from large follicles and, on the other hand, the effects of the opioid agonist FK 33-824 alone or in combination with FSH, PRL or naloxone (NAL) on follicular steroidogenesis were studied. FSH drastically increased β-END-LI output in a dose-dependent fashion. This stimulatory effect of the gonadotrophin was inhibited by the highest dose of P 4 (10 -5 M). The effect of PRL and the steroids added to the cultures on β-END-LI release was negligible. FSH-or PRL-induced P 4 secretion by GCs was essentially abolished by both FK 33-824 and NAL. However, androstenedione (A 4 ) and testosterone output by the cells was greatly potentiated by FK 33-824. In the presence of NAL, FSH or PRL, A 4 release stimulated by FK 33-824 was suppressed to the basal level. Secretion of E 2 was completely free from the influence of FK 33-824 or NAL; only oestrone (E 1 ) output was modulated by them in cultures where FSH or PRL was present. In conclusion, FSH appears to be the key regulator of β-END-LI secretion by porcine granulosa cells. Moreover, steroidogenesis in pig granulosa cells is modulated by opioid peptides acting both alone and by way of interaction with FSH or PRL.opioid peptides / β-endorphin / porcine granulosa cells / steroid secretion Reprod. Nutr. Dev. 40 (2000) 63-75 63

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“…By immunoassay and immunofluorescence techniques, f3-EP has been detected in various nonneuronal tissues such as the gastrointestinal tract (3), pancreas (4), placenta (5), ovary (6), and testis (7). In both intracranial and other tissues, the extent of proteolytic cleavage and posttranslational processing (glycosylation, amidation, and acetylation) varies widely.…”

Section: Introductionmentioning

confidence: 99%

N-acetyl endorphin in rat spermatogonia and primary spermatocytes.

Cheng¹,

Clements²,

Smith³

et al. 1985

J. Clin. Invest.

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In previous reports modest levels of f-endorphin have been found by radioimmunoassay in rat testis, and localized by immunofluorescence to the interstitial cells. We have confirmed these previous reports and extended them by showing that the majority of testicular endorphins are acetylated forms, Nacetyl y-endorphin, N-acetyl a-endorphin, and N-acetyl jBendorphin, 27. In addition, N-acetylated endorphins are not found in interstitial cells, but are confined to spermatogonia and primary spermatocytes.

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Immunoreactive β-endorphin in sheep ovary

Cited by 107 publications

References 16 publications

Pro-opiomelanocortin messenger ribonucleic acid and posttranslational processing of beta endorphin in spleen macrophages.

Pro-opiomelanocortin messenger ribonucleic acid and posttranslational processing of beta endorphin in spleen macrophages.

The physiological role of ?-endorphin in porcine ovarian follicles

N-acetyl endorphin in rat spermatogonia and primary spermatocytes.

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