Immunoreactive Serum Calcitonin and Skeletal Histology in Chronic Renal Failure

Abstract

Serum calcitonin and serum parathyroid hormone (PTH) were measured in 50 patients undergoing regular haemodialysis for end-stage chronic renal failure, and an analysis of osteoclast and osteoblast activities was made in bone biopsies obtained by iliac crest trephine. Osteoclast and osteoblast activities were studied in a multivariate analysis in relation to factors which might reasonably be thought to influence activity, namely serum calcitonin, serum PTH, serum calcium, serum inorganic phosphate, and bone aluminium. Only serum PTH correlated strongly with osteoclast activity (p = 0.0047). Serum PTH correlated also with osteoblast activity (p = 0.0024). Serum inorganic phosphate correlated negatively with osteoblast activity (p = 0.0082). Serum calcitonin did not correlate with osteoclast or osteoblast activities but did correlate strongly with bone aluminium in a multivariate analysis (p = 0.0078). Bone aluminium did not correlate independently with osteoclast or osteoblast activities. This study affirms the implied powerful role of PTH in influencing osteoclast and osteoblast activities in end-stage chronic renal failure.

Effects of Growth Hormone Treatment for Short Stature on Calcium Homeostasis, Bone Mineralisation, and Body Composition

“…Serum l,25-(OH);> vit 1>, was measured by a competitive proteinbinding assay as described by Seshadri et al [17] modified to use a lyophilised preparation of the calf thymus receptor (Immuno Diag nostics, Camperdown, Australia) as the binding protein (assay varia bilities 10 and 15-20%). Calcitonin was measured by RIA using a goat anti-human calcitonin antibody and synthetic human calcitonin for l25I-labelled tracer and standard, with charcoal separation of bound from free radioactivity [18] (assay variabilities 3 and 12%). Serum PTH was measured by an intact-hormone immunoradiometric assay (Nicholls Institute.…”

Somatic mutations in the RET proto‐oncogene in sporadic medullary thyroid carcinoma

“…Base line calcitonin levels were measured by radioimmunoassay (interassay coefficient of variation at a concentration of 200 ng/l serum was 12%) (Furlong et al, 1991) or calcitonin enzyme linked immunoassay (interassay coefficient of variation at a concentration of 100 ng/l serum was 8%) ('calcitonin EASIA', Medgenix Diagnostics, Belgium) and for comparison were divided into three levels: level 1,values were up to twice the upper limit of the normal range; level 2,values were 2-5 times the upper limit of the normal range; and level 3,values were greater than five times the upper limit of the normal range.…”