Immunoreactive LH-RH neurons in the hypothalamus identified by light and fluorescent microscopy

Naik, D. V.

doi:10.1007/bf00222597

Cited by 79 publications

(30 citation statements)

References 22 publications

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“…1973;ZIMMERMAN et al, 1974;NAIK, 1975;BARRY and CARETTE, 1975;SILVERMAN, 1976;SETALO et al, 1976). Other investigators though have been unable to detect LH-RH neuronal somata despite a clear demonstration of their terminals (BAKER et al, 1974;KING et al, 1974;GROSS, 1976).…”

Section: Light Micrographmentioning

confidence: 99%

“…Some authors believe that they are in the arcuate nucleus (ZIMMERMAN, 1974;NAIK, 1975), others that they are located in other hypothalamic region such as the medial preoptic area or the suprachiasmatic nucleus (BARRY et al, 1973;SETALO et al, 1976). This discrepancy may be due to animal species differences, the physiological state and/or the specificity of anti-sera employed for immunohistochemistry.…”

Section: Light Micrographmentioning

confidence: 99%

“…Since some authors have shown immunohistochemically that ependymal processes possess LH-RH reactivity, ependymal processes may play a significant role in the transport of LH-RH synthesized in the neuronal soma in the hypothalamus to the portal capillary (ZIMMERMAN et al, 1974;NAIK, 1975). Exogenous LH-RH injected into the third ventricle was shown by autoradiography to be taken up by ependymal processes (UEMURA et al, 1975;GOLDGEFTER, 1976).…”

Section: Light Micrographmentioning

confidence: 99%

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A Morphological Survey of the Median Eminence: Fluorescence Histochemistry, Electron Microscopy and Immunohistochemistry

Ibata

Watanabe

1977

Arch. Histol. Jap., Arch. Histol. Jpn

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Section: Light Micrographmentioning

confidence: 99%

Section: Light Micrographmentioning

confidence: 99%

Section: Light Micrographmentioning

confidence: 99%

See 1 more Smart Citation

A Morphological Survey of the Median Eminence: Fluorescence Histochemistry, Electron Microscopy and Immunohistochemistry

Ibata

Watanabe

1977

Arch. Histol. Jap., Arch. Histol. Jpn

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“…Recently, a relationship between the glandular pituitary and the pars tuberalis, which had been considered as a simple frontal elongation of the pituitary, was highlighted and its regulatory function within the hypothalamo-pituitary-gonadal axis proposed [8,18,19,35,36,38]. The pars tuberalis and its cytological characteristics and regulatory mechanisms involving LH-RH neurons have also been reported [5, 6, 10, 18, 20, 24-26, 29, 31, 33, 35] along with detailed observations on the distribution of LH-RH nerve fibers in the hypothalamus [2,3,5,10,24,25]. Only Naik [24] has cited the presence of immunopositive LH-RH fibers in the pars tuberalis as well as in the ependymal layer; however, he did not expound upon the significance of these observations.…”

Section: Introductionmentioning

confidence: 99%

“…The pars tuberalis and its cytological characteristics and regulatory mechanisms involving LH-RH neurons have also been reported [5, 6, 10, 18, 20, 24-26, 29, 31, 33, 35] along with detailed observations on the distribution of LH-RH nerve fibers in the hypothalamus [2,3,5,10,24,25]. Only Naik [24] has cited the presence of immunopositive LH-RH fibers in the pars tuberalis as well as in the ependymal layer; however, he did not expound upon the significance of these observations. Recently, the pars tuberalis of the hypophysis was reported as possibly being involved in the photoperiodic regulation of the anterior pituitary hormones [4,[21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Intercellular Communication within the Rat Anterior Pituitary: Immunohistochemical Study on the Relationship between the Hypothalamus, Agranular Cells and GnRH Neurons in the Pars Tuberalis and Anterior Pituitary Gland

Kurita

Shirasawa

Mabuchi

et al. 2004

Acta Histochem. Cytochem

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The pars tuberalis has been generally understood as a simple forward elongation of the glandular pituitary that develops into an architecturally similar structure. In rats, it is also believed that the pars tuberalis completely encircles the infundibular stalk at the ventral portion of hypothalamus. Intact Wistar-Imamichi rats were used in the present study, and frontal and sagittal serial sections of the pituitary gland/brain complex were prepared. Serial sections were stained with hematoxylin-eosin and with antibodies to LH-RH, S-100 protein or LH-RH receptor. Electron microscopic analyses were also conducted. The anatomical end of the epithelial structure of the pars tuberalis was observed at approximately 1 mm behind the optic chiasm. At this point, both the capillaries and the pars tuberalis spread and interdigitated over the median eminence. The pars tuberalis did not, however, completely encircle the pituitary stalk. Electron microscopic observations revealed some profiles of neuroendocrine nerve elements that were also present between the agranular cells of the pars tuberalis. A few LH-RHpositive nerve fibers were initially found just behind the optic chiasm, while the number of LH-RH-positive reactions increased around the capillary vessels. These reactions then decreased significantly and were sparsely distributed both in the diencephalon and in the area near the pituitary stalk where the two separated. LH-RH receptors were observed on S-100 protein-positive cells in the pars tuberalis. The present observations show the significance of the pars tuberalis and its relationship between the anterior pituitary gland and the hypothalamus as organs that possibly form another collaborating mechanism for the regulation of hormone secretion of the anterior pituitary gland.

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Scanning microscopy of pituitary vascular casts

1976

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Vascular casts of the pituitary-median eminence complex from seventeen adult female rabbits were examined with the scanning electron microscope. The results of this study confirm the presence of a single capillary bed common to the entire neurohypophysis. Arterial supply to the rabbit pituitary is only to the neurohypophysis. A direct supply to adenohypophysis was not found. Within the median eminence there are an external and internal capillary plexus. The internal capillary plexus is directed toward the infundibular recess of the third ventricle. It does not receive a direct arterial supply but derives its blood supply from the external plexus before draining to the adenohypophysis. Vessels of the posterior median eminence are confluent with vessels of the infundibular stem. On the basis of these studies, it is proposed that the entire neurohypophysis, not simply the median eminence, serves as the final common pathway to the glandular pituitary. It is also proposed that in the median eminence, vessels are organized to deliver blood containing hypothalamic releasing and inhibiting hormones as well as posterior lobe neural hormones (antidiuretic hormone and oxytocin) to the ventricular surface for subsequent transport to cerebrospinal fluid and distribution to the brain.

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Immunoreactive LH-RH neurons in the hypothalamus identified by light and fluorescent microscopy

Cited by 79 publications

References 22 publications

A Morphological Survey of the Median Eminence: Fluorescence Histochemistry, Electron Microscopy and Immunohistochemistry

A Morphological Survey of the Median Eminence: Fluorescence Histochemistry, Electron Microscopy and Immunohistochemistry

Intercellular Communication within the Rat Anterior Pituitary: Immunohistochemical Study on the Relationship between the Hypothalamus, Agranular Cells and GnRH Neurons in the Pars Tuberalis and Anterior Pituitary Gland

Scanning microscopy of pituitary vascular casts

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