Immunoreactive detection of four mammalian steroids in plants

Simons, R. G.; Grinwich, Daniel L.

doi:10.1139/b89-042

Cited by 39 publications

(29 citation statements)

References 15 publications

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“…1 and Table I). Whereas several studies (31,32) described the presence in plants of the mammalian steroids estradiol, estrone, and testosterone, as well as their physiological effects on growth, development, and flowering, the functional significance of estrogen sulfonation by a plant enzyme remains to be elucidated.…”

Section: Fig 2 Comparison Of the Deduced Amino Acid Sequences Of Bmentioning

confidence: 99%

Inactivation of Brassinosteroid Biological Activity by a Salicylate-inducible Steroid Sulfotransferase from Brassica napus

Rouleau¹,

Marsolais²,

Richard³

et al. 1999

Journal of Biological Chemistry

122

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Recent discoveries from brassinosteroid-deficient mutants led to the recognition that plants, like animals, use steroids to regulate their growth and development. We describe the characterization of one member of a Brassica napus sulfotransferase gene family coding for an enzyme that catalyzes the O-sulfonation of brassinosteroids and of mammalian estrogenic steroids. The enzyme is specific for the hydroxyl group at position 22 of brassinosteroids with a preference for 24-epicathasterone, an intermediate in the biosynthesis of 24-epibrassinolide. Enzymatic sulfonation of 24-epibrassinolide abolishes its biological activity in the bean second internode bioassay. This mechanism of hormone inactivation by sulfonation is similar to the modulation of estrogen biological activity observed in mammals. Furthermore, the expression of the B. napus steroid sulfotransferase genes was found to be induced by salicylic acid, a signal molecule in the plant defense response. This pattern of expression suggests that, in addition to an increased synthesis of proteins having antimicrobial properties, plants respond to pathogen infection by modulating steroid-dependent growth and developmental processes.Many developmental and physiological processes in organisms ranging from fungi to humans are regulated by a small number of steroid hormones. However, until recently the plant kingdom was almost completely excluded from the field of steroid endocrinology. The recent demonstration that the Arabidopsis de-etiolated2 (det2) and the constitutive photomorphogenesis and dwarfism (cpd) mutants are defective in the synthesis of brassinosteroids (BRs) 1 focused attention toward the physiological function of steroids in plants (1, 2). Since these initial discoveries, several other mutants impaired in BR synthesis or perception have been characterized at the molecular level (3-5).BRs have been shown to elicit a broad spectrum of responses including the promotion of cell elongation and cell division, inhibition of de-etiolation in the dark, repression of light-regulated genes in the dark, and repression of stressregulated genes (2, 6, 7). Brassinolide was the first BR isolated and characterized from plants (Fig.

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Section: Fig 2 Comparison Of the Deduced Amino Acid Sequences Of Bmentioning

confidence: 99%

Inactivation of Brassinosteroid Biological Activity by a Salicylate-inducible Steroid Sulfotransferase from Brassica napus

Rouleau¹,

Marsolais²,

Richard³

et al. 1999

Journal of Biological Chemistry

122

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“…They act in various roles like cell membrane constituents and bioregulators such as chemical messengers, vitamins, cytotoxins, and hormones (Barrington, 1979;Sandor and Mehdi, 1979). Steroid molecules have been identifi ed as essential growth regulators in plants as well as in animals using many analytical tools (radioimmunoassay, thinlayer chromatography, mass spectrometry, ultraperformance liquid chromatography, high-performance liquid chromatography) (Geuns, 1978;Jones and Roddick, 1988;Simons and Grinwich, 1989;Janeczko and Skoczowski, 2005;Simersky et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Progesterone and β-Estradiol Stimulate Seed Germination in Chickpea by Causing Important Changes in Biochemical Parameters

Erdal

Dumlupınar

2010

Zeitschrift Für Naturforschung C

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Effects of progesterone and β-estradiol on morphologic (germination velocity, root and shoot length) and biochemical (activities of α-amylase, superoxide dismutase, peroxidase and catalase, H2O2 content, lipid peroxidation) parameters during germination and post-germination stages of chickpea seeds were studied. The seeds germinated at various hormone concentrations (10 - 4, 10 - 6, 10 - 9, 10 - 12, 10 - 15 M) were harvested at the end of the 1st, 3rd, and 5th day. With comparison to the control, these hormones caused an increment in the number of germinating seeds at the end of days 1 and 3 by accelerating the seed germination. Root and shoot lengths were augmented by both hormones at all hormone concentrations tested. The highest elongation was recorded in 10 - 6 M progesterone and 10 - 9 - 10 - 12 M β-estradiol. Similarly, activities of α-amylase and superoxide dismutase were increased by all concentrations of both hormones, and maximum increases were obtained with 10 - 6 M progesterone and 10- 9-10- 12 M β-estradiol. In the case of superoxide dismutase activity, not only the H2O2 content but also the peroxidase and catalase activities increased. Lipid peroxidation decreased depending on an increase in the antioxidant enzyme activities. In the present study, it was demonstrated that progesterone and β-estradiol even at low concentrations increase the germination velocity and resistance to stress conditions by changing the activities of some biochemical pathways.

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“…Tsao denkrehula etal. , 1979Zhou and Tsao 1982;Wang and Tsao 1986;Simons and Grinwich 1989). Significant increases of endogenous estrogens have also been observed during plant reproduction (Kopcewicz 1971(Kopcewicz , 1972aKopcewicz and Chrominski 1972;Kopcewicz et al 1973;Zhou and Tsao 1982).…”

Section: Introductionmentioning

confidence: 99%

The occurrence of estrogens in relation to reproductive processes in flowering plants

Zhang

Yang

Tsao

1991

Sexual Plant Reprod

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Estrogens, a group of steroidal sex hormones, were determined in pollen, styles or whole pistils using a highly sensitive and specific radioimmunoassay (RIA) and found to be present at levels between 10-11 and 10-lo g/g FW. In the style of Lilium davidii Duch., the levels of total estrogen decreased after self-pollination of the open flower. After bud pollination, which partly overcame incompatibility, the levels of both estradiol and total estrogen increased. The levels of estradiol and total estrogen in the pistils of Brassica pekinensis Rupr. increased after compatible pollination during the first 3 days following pollination and decreased thereafter when the data were expressed on a FW basis. These results suggest that estrogens are to some extent associated with the reproductive processes.

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Immunoreactive detection of four mammalian steroids in plants

Cited by 39 publications

References 15 publications

Inactivation of Brassinosteroid Biological Activity by a Salicylate-inducible Steroid Sulfotransferase from Brassica napus

Inactivation of Brassinosteroid Biological Activity by a Salicylate-inducible Steroid Sulfotransferase from Brassica napus

Progesterone and β-Estradiol Stimulate Seed Germination in Chickpea by Causing Important Changes in Biochemical Parameters

The occurrence of estrogens in relation to reproductive processes in flowering plants

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