Immunoreactive antigens of the outer membrane protein of Aeromonas hydrophila, isolated from goldfish, Carassius auratus (Linn.)

Maji, Samata; Mali, Prasenjit; Joardar, Siddhartha Narayan

doi:10.1016/j.fsi.2005.06.003

Cited by 39 publications

(25 citation statements)

References 25 publications

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“…Relative to the vaccines which were delivered alone, the booster dose helped to reduce the cumulative mortality to 10% after Day 10 following the challenge in the present study. Recently, attention has been given to immunoproteomic vaccines such as bacterial OMP (Maji et al 2006) andBF vaccines (Azad et al 1999) to confer immune responses against bacterial and viral pathogens. The OMP vaccines helped to decrease the cumulative mortality to 30% and the OMP with adjuvant to 20% after Aeromonas hydrophila challenge (Thanga Viji et al 2012).…”

Section: Discussionmentioning

confidence: 99%

Vaccination strategies to protect goldfish Carassius auratus against Aeromonas hydrophila infection

Viji¹,

Kumari²,

Velmurugan³

et al. 2013

Dis. Aquat. Org.

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Ornamental goldfish Carassius auratus were treated with whole cell (WC), extracellular product (ECP), outer membrane protein (OMP) and biofilm (BF) vaccines developed from the virulent Aeromonas hydrophila (AHV1; GenBank HQ331525.1) with and without the immunoadjuvant Asparagus racemosus. On various days post-vaccination (dpv), the treated fish were challenged with virulent A. hydrophila. These fish were monitored for survival, growth, specific bacterial reduction, and biochemical, haematological and immunological parameters. C. auratus attained 100% mortality within 7 d in non-vaccinated groups, whereas the vaccines helped to significantly (p ≤ 0.001) increase survival after 25 and 50 dpv. The vaccines with immunoadjuvant (ECP 2 , OMP 2 and BF 2 treatments) helped to reduce the Aeromonas load after the challenge, and serum albumin, globulin and protein levels were significantly (p < 0.01) improved in the OMP 2 -and BF 2 -treated groups. Haemoglobin and red blood cell counts were also significantly improved (p < 0.05) in the vaccinated groups compared to the control group. Additionally, haemagglutination occurred at the 1:12 dilution level in the vaccine plus immunoadjuvant-treated groups. Supplementing the vaccines with immunoadjuvant helped to improve phagocytosis to 54.07%, serum bactericidal activity to 14.6% and the albumin:globulin ratio to 7.6% in BF 2 after 50 dpv. Its positive effect significantly (p < 0.05) increased in vaccinated groups compared to controls. Based on the results, especially with the OMP and BF vaccines, the immunoadjuvant A. racemosus helped to improve the efficiency of the vaccines. This approach will aid in the development of more efficient vaccines against bacterial infections affecting the aquaculture industry. KEY WORDS:Ornamental fish · Biofilm · Outer membrane protein · OMP · Herbal immunoadjuvants Resale or republication not permitted without written consent of the publisher

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Section: Discussionmentioning

confidence: 99%

Vaccination strategies to protect goldfish Carassius auratus against Aeromonas hydrophila infection

Viji¹,

Kumari²,

Velmurugan³

et al. 2013

Dis. Aquat. Org.

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“…The OMPs can induce specific bactericidal antibodies [8][9][10], inhibit the bacterial colonization in hosts [11], and induce cell-mediated immunity [12][13][14]. Many OMPs from different pathogenic bacteria, such as Aeromonas salmonicida [8, [15][16][17], Aeromonas hydrophila [9,[18][19][20][21], Vibrio parahaemolyticus [22], Vibrio alginolyticus [23], Vibrio harveyi [12 -13], Edwardsiella ictaluri [10], and Edwardsiella tarda [14,24], have been identified as effective protective antigens. Some OMPs mixtures extracted from one pathogenic bacterial strain have been proved to provide cross protection for fish against several other bacterial strains or species [15,20], suggesting that different pathogenic bacterial species may share some similar or conserved OMP antigens.…”

Section: Introductionmentioning

confidence: 99%

Enhancement of protective immunity in European eel (<italic>Anguilla anguilla</italic>) against <italic>Aeromonas hydrophila</italic> and <italic>Aeromonas sobria</italic> by a recombinant <italic>Aeromonas</italic> outer membrane protein

Guan¹,

Xiong²,

Huang³

et al. 2011

ABBS

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To develop a vaccine, which can simultaneously prevent the diseases caused by various pathogenic bacteria in fish, we try to find a conserved outer membrane protein (OMP) antigen from different bacterial pathogens. In this study, an OMP fragment of 747 bp (named as Omp-G), which was highly conserved in seven Aeromonas OMP sequences from the NCBI database, was amplified by PCR from one Aeromonas sobria strain (B10) and two Aeromonas hydrophila strains (B27 and B33) with the designed specific primers. The sequence was cloned into pGEX-2T (6 3 His-tag) vector, expressed in Escherichia coli system, and then the recombinant protein (named as rOmp-G) was purified with nickel chelating affinity chromatography. The purified rOmp-G showed a good immunogenicity in rabbits and well-conserved characteristics in these three pathogens by enzyme-linked immunosorbed assay. Furthermore, the rOmp-G also showed good immunogenicity in eels (Anguilla anguilla) for eliciting significantly increased specific antibodies (P < 0.01), and providing higher protection efficiencies (P < 0.05) after the pathogens challenge. The values of the relative percent survival in eels were 70% and 50% for two A. hydrophila strain challenge, and 75% for A. sobria strain challenge. This is the first report of a potential vaccination in eels that simultaneously provide protectiveness against different Aeromonas pathogens with a conserved partial OMP.

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“…). Several studies on the development of protein‐based vaccines against A. hydrophila have been conducted (Rahman & Kawai ; Maji, Mali & Joardar ; Khushiramani et al . ; Maiti et al .…”

Section: Discussionmentioning

confidence: 99%

“…To solve this problem, it has been reported that the use of a wide range of immunization with a vaccine that consists of the common protective antigens of A. hydrophila may be effective (Ni et al 2010). Several studies on the development of protein-based vaccines against A. hydrophila have been conducted (Rahman & Kawai 2000;Maji, Mali & Joardar 2006;Khushiramani et al 2007;Maiti et al 2009). However, it has been reported that these were not adequately evaluated whether they provide protection against challenge with heterologous strains.…”

Section: Discussionmentioning

confidence: 99%

Development and validation of glycoprotein‐based native‐subunit vaccine for fish against Aeromonas hydrophila

Çiftci

Onuk

Çiftçi

et al. 2016

Journal of Fish Diseases

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Aeromonas hydrophila is known to be causative agent of an infection named as Bacterial haemorrhagic septicaemia or red pest in freshwater fish. The aim of this study was to develop and validate the glycoprotein-based fish vaccine against Aeromonas hydrophila. For this aim, after identification and characterization of A. hydrophila isolates from fish farms, one A. hydrophila isolate was selected as vaccine strain. Antigenic glycoproteins of this vaccine strain were determined by Western blotting and glycan detection kit. The connection types of these glycoproteins were examined by glycoprotein differentiation kit. Two glycoproteins, molecular weights of 19 and 38 kDa, with SNA connection type were selected for use in vaccination trials. After their purification by SNA-specific lectin and size-exclusion chromatography, protection studies with purified proteins were performed. For challenge trials, four experimental fish groups were designated: Group I (with montanide), Group II (with montanide and ginseng), Group III [with Al(OH)3 ] and Group IV [with Al(OH)3 and ginseng]. The survival ratings of fish were determined, and protection was calculated as 21.56%, 29.41%, 69.83% and 78.88% in groups I, II, III and IV, respectively. In conclusion, A. hydrophila glycoproteins with Al(OH)3 and ginseng could be used as a safe and effective vaccine for fish.

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Immunoreactive antigens of the outer membrane protein of Aeromonas hydrophila, isolated from goldfish, Carassius auratus (Linn.)

Cited by 39 publications

References 25 publications

Vaccination strategies to protect goldfish Carassius auratus against Aeromonas hydrophila infection

Vaccination strategies to protect goldfish Carassius auratus against Aeromonas hydrophila infection

Enhancement of protective immunity in European eel (<italic>Anguilla anguilla</italic>) against <italic>Aeromonas hydrophila</italic> and <italic>Aeromonas sobria</italic> by a recombinant <italic>Aeromonas</italic> outer membrane protein

Development and validation of glycoprotein‐based native‐subunit vaccine for fish against Aeromonas hydrophila

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Immunoreactive antigens of the outer membrane protein of Aeromonas hydrophila, isolated from goldfish, Carassius auratus (Linn.)

Cited by 39 publications

References 25 publications

Vaccination strategies to protect goldfish Carassius auratus against Aeromonas hydrophila infection

Vaccination strategies to protect goldfish Carassius auratus against Aeromonas hydrophila infection

Enhancement of protective immunity in European eel (&lt;italic&gt;Anguilla anguilla&lt;/italic&gt;) against &lt;italic&gt;Aeromonas hydrophila&lt;/italic&gt; and &lt;italic&gt;Aeromonas sobria&lt;/italic&gt; by a recombinant &lt;italic&gt;Aeromonas&lt;/italic&gt; outer membrane protein

Development and validation of glycoprotein‐based native‐subunit vaccine for fish against Aeromonas hydrophila

Contact Info

Product

Resources

About

Enhancement of protective immunity in European eel (<italic>Anguilla anguilla</italic>) against <italic>Aeromonas hydrophila</italic> and <italic>Aeromonas sobria</italic> by a recombinant <italic>Aeromonas</italic> outer membrane protein