Immunoproteomics of Helicobacter pylori infection and relation to gastric disease

Haas, Gaby; Karaali, Galip; Ebermayer, Karl; Metzger, Wolfram; Lamer, Stephanie; Zimny‐Arndt, Ursula; Diescher, Susanne; Goebel, Ursula; Vogt, Konstanze; Roznowski, A B; Wiedenmann, B.; Meyer, Thomas F.; Aebischer, Toni; Jungblut, Peter R.

doi:10.1002/1615-9861(200203)2:3<313::aid-prot313>3.0.co;2-7

Cited by 156 publications

(129 citation statements)

References 26 publications

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“…In addition, a few unidentified antigenic bands, with molecular weights of 18, 34 and 39.5 kDa, have been reported to possess good diagnostic value (Andersen and Espersen 1992;Galmiche et al, 2000;Haas et al, 2002). Lin et al (2007) detected seven proteins, with relative molecular masses of 76 KDa (flagellar hook protein), 74 KDa [flagellar hook-associated protein (FliD)], 67 KDa (molecular chaperone DnaK), 61 KDa (urease β subunit), 53 KDa (flagellin A), 53 KDa (flagellin B), and 51 KDa (serine protease (HtrA), in serum samples from patients with duodenal ulcers and gastric cancer.…”

Section: Discussionmentioning

confidence: 70%

Antigenic Proteins of Helicobacter pylori of Potential Diagnostic Value

Khalilpour

Agilan²,

Lee³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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Helicobacter pylori antigen was prepared from an isolate from a patient with a duodenal ulcer. Serum samples were obtained from culture-positive H. pylori infected patients with duodenal ulcers, gastric ulcers and gastritis (n=30). As controls, three kinds of sera without detectable H. pylori IgG antibodies were used: 30 from healthy individuals without history of gastric disorders, 30 from patients who were seen in the endoscopy clinic but were H. pylori culture negative and 30 from people with other diseases. OFF-GEL electrophoresis, SDS-PAGE and Western blots of individual serum samples were used to identify protein bands with good sensitivity and specificity when probed with the above sera and HRP-conjugated anti-human IgG. Four H. pylori protein bands showed good (≥ 70%) sensitivity and high specificity (98-100%) towards anti-Helicobacter IgG antibody in culturepositive patients sera and control sera, respectively. The identities of the antigenic proteins were elucidated by mass spectrometry. The relative molecular weights and the identities of the proteins, based on MALDI TOF/ TOF, were as follows: CagI (25 kDa), urease G accessory protein (25 kDa), UreB (63 kDa) and proline/pyrroline-5-carboxylate dehydrogenase (118 KDa). These identified proteins, singly and/or in combinations, may be useful for diagnosis of H. pylori infection in patients.

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Section: Discussionmentioning

confidence: 70%

Antigenic Proteins of Helicobacter pylori of Potential Diagnostic Value

Khalilpour

Agilan²,

Lee³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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“…12, which is published as supporting information on the PNAS web site). These results are consistent with other reports that have failed to identify BabA or BabB as immunodominant antigens in humans (25,26). Taken together, the findings that rhesus-derived strains of H. pylori and most J166 output strains have two copies of babB suggest to us that BabB may function as an adhesin and that overexpression of BabB is advantageous.…”

Section: Discussionmentioning

confidence: 99%

Modification of Helicobacter pylori outer membrane protein expression during experimental infection of rhesus macaques

Solnick

Hansen

Salama

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

214

216

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Clinical isolates of Helicobacter pylori show marked diversity, which may derive from genomic changes that occur during the often lifelong association of the bacterium with its human host. We used the rhesus macaque model, together with DNA microarrays, to examine genomic changes in H. pylori that occur early during experimental infection. Microarray analysis showed that H. pylori recovered from challenged macaques had deleted babA, a member of a large family of paralogous outer membrane proteins (OMPs) that mediates attachment of H. pylori to the Lewis B blood group antigen on gastric epithelium. In some cases the babA gene was replaced by babB, an uncharacterized OMP that is closely related to babA. In other cases the babA gene was present but was not expressed because of alteration in dinucleotide CT repeats in the 5 coding region. In either case, strains lacking babA did not adhere to Lewis B, which is expressed on macaque gastric epithelium. Absence of babA and duplication of babB was also seen in H. pylori isolates derived from human clinical samples, suggesting that this gene conversion event is not unique to experimentally infected rhesus monkeys. These results demonstrate in real time with a relevant animal model that H. pylori regulates OMP expression in vivo by using both antigenic variation and phase variation. We suggest that changes in babA and babB after experimental infection of macaques represent a dynamic response in the H. pylori outer membrane that facilitates adherence to the gastric epithelium and promotes chronic infection.

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“…Among the currently available techniques, the combination of proteomics with serology (immunoproteomics (30 -34)) is largely being used to screen for panels of biomarkers and therapeutic targets in patients with infectious diseases (including SC (27,31,35,36)), cancer, or autoimmune disorders (32,(37)(38)(39)(40)(41). This discipline permits characterization of the immunome of a (micro)organism, defined as the subset of the proteome of a (micro)organism that acts as a target for the immune system (42).…”

Section: Not Only Does Systemic Candidiasis (Sc)mentioning

confidence: 97%

Decoding Serological Response to Candida Cell Wall Immunome into Novel Diagnostic, Prognostic, and Therapeutic Candidates for Systemic Candidiasis by Proteomic and Bioinformatic Analyses

Pitarch

Jiménez

Nombela

et al. 2006

Molecular & Cellular Proteomics

129

123

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In an effort to bring novel diagnostic and prognostic biomarkers or even potential targets for vaccine design for systemic candidiasis (SC) into the open, a systematic proteomic approach coupled with bioinformatic analysis was used to decode the serological response to Candida wall immunome in SC patients. Serum levels of IgG antibodies against Candida wall-associated proteins (proteins secreted from protoplasts in active wall regeneration, separated by two-dimensional gel electrophoresis, and identified by mass spectrometry) were measured in 45 SC patients, 57 non-SC patients, and 61 healthy subjects by Western blotting. Two-way hierarchical clustering and principal component analysis of their serum anti-Candida wall antibody expression patterns discriminated SC patients from controls and highlighted the heterogeneity of their expression profiles. Multivariate logistic regression models demonstrated that high levels of antibodies against glucan 1,3-␤-glucosidase (Bgl2p) and the antiwall phosphoglycerate kinase antibody seropositivity were the only independent predictors of SC. Receiver operating characteristic curve analysis revealed no difference between their combined evaluation and measurement of anti-Bgl2p antibodies alone. In a logistic regression model adjusted for known prognostic factors for mortality, SC patients with high anti-Bgl2p antibody levels or a positive anti-wall enolase antibody status, which correlated with each other, had a reduced 2-month risk of death. After controlling for each other, only the seropositivity for anti-wall enolase antibodies was an independent predictor of a lower risk of fatality, supporting that these mediated the protective effect. No association between serum anti-cytoplasmic enolase antibody levels and outcomes was established, suggesting a specific mechanism of enolase processing during wall biogenesis. We conclude that serum anti-Bgl2p antibodies are a novel accurate diagnostic biomarker for SC and that, at high levels,

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Immunoproteomics of Helicobacter pylori infection and relation to gastric disease

Cited by 156 publications

References 26 publications

Antigenic Proteins of Helicobacter pylori of Potential Diagnostic Value

Antigenic Proteins of Helicobacter pylori of Potential Diagnostic Value

Modification of Helicobacter pylori outer membrane protein expression during experimental infection of rhesus macaques

Decoding Serological Response to Candida Cell Wall Immunome into Novel Diagnostic, Prognostic, and Therapeutic Candidates for Systemic Candidiasis by Proteomic and Bioinformatic Analyses

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