Immunoproteomics Methods and Techniques

Fulton, Kelly M.; Baltat, Isabel; Twine, Susan M.

doi:10.1007/978-1-4939-9597-4_2

Cited by 14 publications

(17 citation statements)

References 294 publications

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“…Several studies have focused on infection-associated antigens that trigger host immune responses. This approach is named immunoproteomics and can be achieved by combining 2-DE protein separation, Western blotting, and MS [102]. Immunoblotting was performed on P. aeruginosa strains isolated from cystic fibrosis (CF) patients [41].…”

Section: Proteomics For Host-pathogen Studiesmentioning

confidence: 99%

Exoproteomics for Better Understanding Pseudomonas aeruginosa Virulence

Sauvage

Hardouin

2020

Toxins

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Pseudomonas aeruginosa is the most common human opportunistic pathogen associated with nosocomial diseases. In 2017, the World Health Organization has classified P. aeruginosa as a critical agent threatening human health, and for which the development of new treatments is urgently necessary. One interesting avenue is to target virulence factors to understand P. aeruginosa pathogenicity. Thus, characterising exoproteins of P. aeruginosa is a hot research topic and proteomics is a powerful approach that provides important information to gain insights on bacterial virulence. The aim of this review is to focus on the contribution of proteomics to the studies of P. aeruginosa exoproteins, highlighting its relevance in the discovery of virulence factors, post-translational modifications on exoproteins and host-pathogen relationships.

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Section: Proteomics For Host-pathogen Studiesmentioning

confidence: 99%

Exoproteomics for Better Understanding Pseudomonas aeruginosa Virulence

Sauvage

Hardouin

2020

Toxins

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“…Immunoproteomics is an extension of proteomics, which permits specific elucidation of proteins based on immunoreactivity (Fulton et al 2019 ). In procedure of immunoproteomics, 2-D gel blots are probed with antiserum collected from host post infection.…”

Section: Resultsmentioning

confidence: 99%

Immunoproteomic analysis of Clostridium botulinum type B secretome for identification of immunogenic proteins against botulism

et al. 2021

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Objectives To identify immunogenic proteins of C. botulinum type B secretome by immunoproteomic analysis. Results In the present study, an attempt was made to elucidate the vaccine candidates/diagnostic molecules against botulism using immuno proteomic approach. C. botulinum type B secretome was elucidated when it was grown in TPGY as well as CMM media. Predominant 51 proteins were identified in both the media using 2-DE and mass spectrometry analysis. 2D gels (CMM & TPGY) were probed with respected proteins mice antiserum and obtained 17 and 10 immunogenic proteins in TPGY as well as CMM media respectively. Hypothetical protein CLOSPO_00563, ornithine carbamoyl transferase, FlaA, molecular chaperone GroEL and secreted protease proteins were found as the common immuno dominant proteins in both media. Polyclonal Antibodies raised against C. botulinum types A and E showed cross-reactivity with secretome C. botulinum type B at the lowest dilution (1:1000) but did not show cross reactivity with highest dilution (1:30,000) with C. botulinum type B secretome. Polyclonal antibodies against C. botulinum type F secretome did not show cross reactivity with C. botulinum type B secretome. Conclusions Identified immunogenic proteins can be used as vaccine candidates and diagnostic markers for the infant and wound botulism but common immunogenic proteins may be the best vaccine candidate molecule for development of vaccine as well as diagnostic system against the infant and wound botulism. Supplementary Information The online version contains supplementary material available at 10.1007/s10529-021-03091-4.

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“…MLAA-34 is one of the newly identified monocytic leukemia-associated antigens ( 11 , 19 ). This gene is homologous to the known human CAB39L gene and has been confirmed to be a novel splice variant of CAB39L ( 5 , 9 ).…”

Section: Discussionmentioning

confidence: 99%

C59T mutation in exon 2 of monocytic leukemia-associated antigen-34 gene indicates a high risk of recurrence of acute myeloid leukemia

Lei

Chen

et al. 2017

Oncology Letters

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Monocytic leukemia-associated antigen-34 (MLAA-34) is a novel monocytic leukemia-associated antigen and a candidate oncogene. The aim of the present study was to investigate the involvement of the MLAA-34 gene in acute myeloid leukemia (AML). MLAA-34 expression level, chromosome location, gene copy number and single nucleotide polymorphisms (SNPs) of 40 patients with AML and 5 healthy volunteers were analyzed by reverse transcription-polymerase chain reaction, fluorescence in situ hybridization and DNA sequencing. The effects of MLAA-34 mutation on overall survival (OS) and progression-free survival (PFS) of patients with AML were also analyzed. MLAA-34 was significantly upregulated in patients with AML when compared with volunteer controls, and this upregulation was associated with a C59T SNP site located in the second exon of MLAA-34. MLAA-34 was mapped to 13q14.2 and no translocation was observed in patients with AML. In addition, this SNP site is affinitive to the well-known molecular markers of AML, including Fms-like tyrosine kinase 3 and DNA methyltransferase 3A, as well as extramedullary lesions, periphery leukocyte numbers, remission and cytogenetic abnormalities of patients with AML. Patients with AML with MLAA-34 C59T mutations had significantly shorter OS and PFS times compared with that of patients without C59T mutations. The present findings indicated that the MLAA-34 C59T mutation was a high-risk factor for recurrence of AML, and may be a candidate target for AML therapy.

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Immunoproteomics Methods and Techniques

Cited by 14 publications

References 294 publications

Exoproteomics for Better Understanding Pseudomonas aeruginosa Virulence

Exoproteomics for Better Understanding Pseudomonas aeruginosa Virulence

Immunoproteomic analysis of Clostridium botulinum type B secretome for identification of immunogenic proteins against botulism

C59T mutation in exon 2 of monocytic leukemia-associated antigen-34 gene indicates a high risk of recurrence of acute myeloid leukemia

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