Immunoproteomic to Analysis the Pathogenicity Factors in Leukopenia Caused by Klebsiella Pneumonia Bacteremia

Abstract

Incidences of leukopenia caused by bacteremia have increased significantly and it is associated with prolonged hospital stay and increased cost. Immunoproteomic is a promising method to identify pathogenicity factors of different diseases. In the present study, we used immunoproteomic to analysis the pathogenicity factors in leukopenia caused by Klebsiella Pneumonia bacteremia. Approximately 40 protein spots localized in the 4 to 7 pI range were detected on two-dimensional electrophoresis gels, and 6 differentially expressed protein spots between 10 and 170 kDa were identified. Pathogenicity factors including S-adenosylmethionine synthetase, pyruvate dehydrogenase, glutathione synthetase, UDP-galactose-4-epimerase, acetate kinase A and elongation factor tu (EF-Tu). In validation of the pathogenicity factor, we used western blotting to show that Klebsiella pneumonia had higher (EF-Tu) expression when they accompanied by leukopenia rather than leukocytosis. Thus, we report 6 pathogenicity factors of leukopenia caused by Klebsiella pneumonia bacteremia, including 5 housekeeping enzymes and EF-Tu. We suggest EF-Tu could be a potential pathogenicity factor for leukopenia caused by Klebsiella pneumonia.

From the wound to the bench: exoproteome interplay between wound-colonizing Staphylococcus aureus strains and co-existing bacteria

“…Similarly, the elongation factor (EF) Tu, a guanosine nucleotide binding protein important for protein synthesis, was detected on the surface of Mycoplasma pneumoniae and Lactobacillus johnsonii , where it was involved in fibronectin- and mucin-binding, respectively [ 59 , 60 ]. Furthermore, an immunoproteomic study of bacteremia showed that six housekeeping enzymes and EF-Tu were linked to leukopenia caused by Klebsiella pneumonia [ 61 ]. Altogether, these findings imply that proteins, which were originally considered to be restricted to the cytoplasm, may also be important for virulence enhancement and invasive disease [ 50 , 62 ].…”

The Diverse Functional Roles of Elongation Factor Tu (EF-Tu) in Microbial Pathogenesis

“…Antibodies against EF-Tu have also been detected in a range of natural infections, including those caused by Mycoplasma hyopneumoniae (Pinto et al, 2007), Chlamydia trachomatis (Sanchez-Campillo et al, 1999) and K . pneumonia (Liu et al, 2014). Recombinant EF-Tu (rEF-Tu) from Mycoplasma ovipneumoniae induces an immune response in mice, increasing levels of IgG, TNF-α, IFN-γ, IL-12(p70), IL-4, IL-5, and IL-6.…”

“…Bacterial infections can also lead to a reduction in white blood cell (WBC) counts in infected hosts leading to leukopenia. Isolates of K. pneumoniae from patients with leukopenia express higher levels of EF-Tu compared with K. pneumoniae isolates from patients with leucocytosis (Liu et al, 2014) suggesting that EF-Tu may be a pathogenicity factor in K. pneumonia -based leukopenia (Liu et al, 2014). Interestingly, EF-Tu is upregulated in Mycobacterium species that have been phagocytosed by macrophages.…”

Effect of Porphyromonas gingivalis infection on leukocyte count in rat model of diabetes mellitus

“…The host responded by increasing the number of leukocyte cells in the bloodstream as a defense against Pg. 31 Moreover, the Pg virulence factors released protease enzymes reducing the function of the intercellular endothelial junctional cadherin and platelet endothelial cell adhesion molecule. The virulence factors also enhanced permeability of vascular endothelial and gingival epithelium.…”