Immunoproteomic Identification of Noncarbohydrate Antigens Eliciting Graft‐Specific Adaptive Immune Responses in Patients with Bovine Pericardial Bioprosthetic Heart Valves

Generation of glycans depleted decellularized porcine pericardium, using digestive enzymatic supplements and enzymatic mixtures for food industry

“…Sugars have been reported as the major key players in xenoimmunogenicity; nevertheless, other organic compounds might play a major role as well. In addition to carbohydrates, proteins have also been investigated as potential xenoantigens 42 . Nonetheless, the in vivo testing of glycans‐depleted xenogeneic matrices might help in a better understanding of xenografts rejection.…”

“…This research study represents a proof of concept; the results reported in this In addition to carbohydrates, proteins have also been investigated as potential xenoantigens. 42 Nonetheless, the in vivo testing of glycans-depleted xenogeneic matrices might help in a better understanding of xenografts rejection.…”

GTKO rabbit: A novel animal model for preclinical assessment of decellularized xenogeneic grafts via in situ implantation

“…Graft-specific IgG and IgM levels were detected using indirect ELISA [ [24] , [25] , [26] , [27] , [28] ]. The PCB homogenate contains various immunogenic substances that may be contained in the material, including proteins, polysaccharides, and even small molecular substances such as chemical reagent residues.…”

Immunogenicity of decellularized extracellular matrix scaffolds: a bottleneck in tissue engineering and regenerative medicine

“…Additional to immunologic concerns related to glycan antigens, xenogeneic dECM could induce the immune response due to some of their protein constituents. In a study on the immunogenicity of bovine bioprosthetic heart valves, 19 protein antigens, including one ECM protein (Hyaluronan and proteoglycan link protein 3), provoked human antibody response [148]. Moreover, collagen structure comprises triple helix and non-helical regions named telopeptides.…”