Immunoproteomic Analysis of Antibody Response of Rabbit Host Against Heat-Killed Francisella tularensis Live Vaccine Strain

Gaur, Ritu; Alam, Syed Imteyaz; Kamboj, Dev Vrat

doi:10.1007/s00284-017-1217-y

Cited by 8 publications

(7 citation statements)

References 33 publications

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“…Since rabbits are a vector of tularemia and are naturally susceptible to this pathogen, the immune reaction following infection was studied previously by evaluating anti-bacteria antibody titers, changes in clinical and hematological parameters and more 21–23 . Others have also further analyzed the antibody responses towards specific proteins and towards the LPS moieties 24,25 . Here, we took advantage of the fact that rabbits can tolerate infection with live LVS 26 in order to generate an immunization protocol that involved repeated exposures of rabbits to this strain, in order to elicit a strong immune response.…”

Section: Resultsmentioning

confidence: 99%

Inhibition of Francisella tularensis phagocytosis using a novel anti-LPS scFv antibody fragment

Mechaly

Elia

Alcalay

et al. 2019

Sci Rep

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Francisella tularensis ( Ft ), the causative agent of lethal tularemia, is classified as a category A biological warfare threat agent. While Ft infection is treatable by antibiotics, many failed antibiotic treatments were reported, highlighting the need for effective new treatments. It has been demonstrated that binding of antibody-coated bacteria to the Fc receptor located on phagocytic cells is a key process needed for efficient protection against Ft . Yet, Ft utilizes the same receptor to enter the phagocytic cells in order to escape the immune system. To address the question whether an anti- Ft LPS antibody lacking the ability to bind the Fc receptor may inhibit the entry of Ft into host cells, a soluble scFv (TL1-scFv) was constructed from an anti Ft -LPS antibody (TL1) that was isolated from an immune single-chain (scFv) phage-display library. Bacterial uptake was assessed upon infection of macrophages with Ft live attenuated strain (LVS) in the presence of either TL1 or TL1-scFv. While incubation of LVS in the presence of TL1 greatly enhanced bacterial uptake, LVS uptake was significantly inhibited in the presence of TL1-scFv. These results prompt further experiments probing the therapeutic efficacy of TL1-scFv, alone or in combination with antibiotic treatment.

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Section: Resultsmentioning

confidence: 99%

Inhibition of Francisella tularensis phagocytosis using a novel anti-LPS scFv antibody fragment

Mechaly

Elia

Alcalay

et al. 2019

Sci Rep

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“…That led to recognising 28 immunoreactive proteins after performing MS/MS. Then, the rabbit immunoproteome of F. tularensis was compared with those previously reported and, of the above proteins, 12 were recognised by human serum [ 143 ]. Nine proteins were found to be immunogenic in rabbits, mice and humans; of these, eight were new ones.…”

Section: Proteomics Applications In Zoonotic Infectionsmentioning

confidence: 99%

“…Nine proteins were found to be immunogenic in rabbits, mice and humans; of these, eight were new ones. The immunoreactive proteins identified in the study may be used in the design and development of protein subunit vaccines against the disease [ 143 ].…”

Section: Proteomics Applications In Zoonotic Infectionsmentioning

confidence: 99%

Applied Proteomics in ‘One Health’

Katsarou

Billinis

Galamatis³

et al. 2021

Proteomes

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‘One Health’ summarises the idea that human health and animal health are interdependent and bound to the health of ecosystems. The purpose of proteomics methodologies and studies is to determine proteins present in samples of interest and to quantify changes in protein expression during pathological conditions. The objectives of this paper are to review the application of proteomics technologies within the One Health concept and to appraise their role in the elucidation of diseases and situations relevant to One Health. The paper develops in three sections. Proteomics Applications in Zoonotic Infections part discusses proteomics applications in zoonotic infections and explores the use of proteomics for studying pathogenetic pathways, transmission dynamics, diagnostic biomarkers and novel vaccines in prion, viral, bacterial, protozoan and metazoan zoonotic infections. Proteomics Applications in Antibiotic Resistance part discusses proteomics applications in mechanisms of resistance development and discovery of novel treatments for antibiotic resistance. Proteomics Applications in Food Safety part discusses the detection of allergens, exposure of adulteration, identification of pathogens and toxins, study of product traits and characterisation of proteins in food safety. Sensitive analysis of proteins, including low-abundant ones in complex biological samples, will be achieved in the future, thus enabling implementation of targeted proteomics in clinical settings, shedding light on biomarker research and promoting the One Health concept.

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“…This has led to the development of several methods, from the detection of the total protein pattern on the 2D blots prior and/or after the immunodetection (e.g., in [ 64 ]) to the partial blotting process, in which the very same 2D gel is used, both for generating the 2D reference pattern for MS identification and the 2D blot for immunodetection [ 65 ]. In the immune responses to pathogens, work has been carried out on bacteria [ 66 , 67 , 68 , 69 , 70 ], fungi [ 71 , 72 , 73 ], and parasites [ 74 , 75 ]. Such studies have always reached their initial goal of providing the major immunogens from the pathogen under study, and have sometimes resulted in valuable clinical advances [ 71 , 76 , 77 ].…”

Section: When An Unpredictable Subset Of Proteins Is To Be Analyzementioning

confidence: 99%

What Room for Two-Dimensional Gel-Based Proteomics in a Shotgun Proteomics World?

2020

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Two-dimensional gel electrophoresis was instrumental in the birth of proteomics in the late 1980s. However, it is now often considered as an outdated technique for proteomics—a thing of the past. Although this opinion may be true for some biological questions, e.g., when analysis depth is of critical importance, for many others, two-dimensional gel electrophoresis-based proteomics still has a lot to offer. This is because of its robustness, its ability to separate proteoforms, and its easy interface with many powerful biochemistry techniques (including western blotting). This paper reviews where and why two-dimensional gel electrophoresis-based proteomics can still be profitably used. It emerges that, rather than being a thing of the past, two-dimensional gel electrophoresis-based proteomics is still highly valuable for many studies. Thus, its use cannot be dismissed on simple fashion arguments and, as usual, in science, the tree is to be judged by the fruit.

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Immunoproteomic Analysis of Antibody Response of Rabbit Host Against Heat-Killed Francisella tularensis Live Vaccine Strain

Cited by 8 publications

References 33 publications

Inhibition of Francisella tularensis phagocytosis using a novel anti-LPS scFv antibody fragment

Inhibition of Francisella tularensis phagocytosis using a novel anti-LPS scFv antibody fragment

Applied Proteomics in ‘One Health’

What Room for Two-Dimensional Gel-Based Proteomics in a Shotgun Proteomics World?

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