Immunoprotective response induced by recombinant glycoprotein D in the BALB/c respiratory mouse model of Equid alphaherpesvirus 1 infection

Fuentealba, Nadia Analía; Sguazza, Guillermo Hernán; Zanuzzi, Carolina Natalia; Bravi, María Emilia; Scrochi, Mariela Rita; Valera, Alejandro Rafael; Corva, Santiago Gerardo; Gimeno, Eduardo Juan; Pecoraro, Marcelo Ricardo Ítalo; Galosi, Cecília Mônica

doi:10.1016/j.ram.2018.05.004

Cited by 2 publications

(3 citation statements)

References 38 publications

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“…Nanoparticles can be efficiently engulfed by DCs and induce DC maturation and cytokine release, activating antigen-specific immune responses and enhancing T cell proliferation, IFN-γ production and generation of cytotoxic T lymphocytes (Dudek et al 2018). The traditional adjuvant ISA206 is a water-oil-water emulsion, which may maintain the native structures of antigen better than water-oil adjuvants (Fuentealba et al 2019;G.F. El-Bagoury1 and Darwish2 2010;Zhugunissov et al 2018).…”

Section: Discussionmentioning

confidence: 99%

Polystyrene nanoparticles induce stronger cellular and more durable humoral immune responses against an inactivated bovine herpesvirus 1 isolate

Liu

Xin

Zhang

et al. 2020

Preprint

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The inactivated bovine herpesvirus type 1(BoHV-1) vaccines are generally safe and suitable for use in dairy and pregnant cattle, but induces weaker cellular immune responses and shorter antibody responses compared with the modified live virus vaccine. In this study, we used polystyrene (PS) nanoparticles (100 nm) as a carrier for purified inactivated broken BoHV-1 to improve cellular and humoral immune responses compared with the traditional inactivated vaccine. Mice were injected intramuscularly with the inactivated complex mixed with ISA206 adjuvant. Transmission electron microscopy showed that the PS nanoparticles displayed broken BoHV-1 on their surfaces. After validation of BoHV-1 and gB gC gD gE tegument proteins, it proved that the BoHV-conjugated PS nanoparticles induced higher-titer and more durable antibody responses. The inactivated BoHV-PS nanoparticle complex elicited neutralizing antibodies (titer ~2 6 ) in 5 weeks post-immunization in mice. The CD4/CD8 ratio was higher in mice immunized with PS nanoparticles compared with other groups. However, this ratio reached its maximum 1 week later than in mice immunized with ISA206+BoHV-1 or BoHV-1. Levels of interleukin (IL)-4, IL-6, and interferon-γ in followed similar patterns. In conclusion, this pilot study demonstrated that PS nanoparticles can adjuvant inactivated BoHV-1 vaccines, enhancing both cell-mediated immune responses and the duration of antibody responses. This study provides the foundation for a new development platform for inactivated vaccines, which can elicit potent cellular and humoral immune responses in animals and humans.The inactivated bovine herpesvirus type 1(BoHV-1) vaccines are generally safe and suitable for use in dairy and pregnant cattle, but induces weaker cellular immune responses and shorter antibody responses compared with the modified live virus vaccine. In this study, we used polystyrene (PS) nanoparticles (100 nm) as a carrier for purified inactivated broken BoHV-1 to improve cellular and humoral immune responses compared with the traditional inactivated vaccine. Mice were injected intramuscularly with the inactivated complex mixed with ISA206 adjuvant. Transmission electron microscopy showed that the PS nanoparticles displayed broken BoHV-1 on their surfaces. After validation of BoHV-1 and gB gC gD gE tegument proteins, it proved that the BoHV-conjugated PS nanoparticles induced higher-titer and more durable antibody responses. The inactivated BoHV-PS nanoparticle complex elicited neutralizing antibodies (titer ~2 6 ) in 5 weeks post-immunization in mice. The CD4/CD8 ratio was higher in mice immunized with PS nanoparticles compared with other groups. However, this ratio reached its maximum 1 week later than in mice immunized with ISA206+BoHV-1 or BoHV-1. Levels of interleukin (IL)-4, IL-6, and interferon-γ in followed similar patterns. In conclusion, this pilot study demonstrated that PS nanoparticles can adjuvant inactivated BoHV-1 vaccines, enhancing both cell-mediated immune responses and the duration of antibody responses. This study provides the foundation for a new development platform for inactivated vaccines, which can elicit potent cellular and humoral immune responses in animals and humans.

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Section: Discussionmentioning

confidence: 99%

Polystyrene nanoparticles induce stronger cellular and more durable humoral immune responses against an inactivated bovine herpesvirus 1 isolate

Liu

Xin

Zhang

et al. 2020

Preprint

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“…The virus encodes 12 surface glycoproteins of which glycoprotein B (gB) and glycoprotein C (gC) play important role in the entry of virions into the host cell by making contact with proteoglycans present on the cell surface for adsorption, penetration and cell to cell spread of virions inside the host (Shukla and Spear, 2001;Spear and Longnecker, 2003). The gD has shown to elicit protective mucosal immune response thereby inhibits the initial virus attachment and entry into the respiratory epithelial cells (Fuentealba et al, 2019). These glycoproteins are thus important targets for the immune system and have been experimentally used for immunization of animals against the homologous virus either individually or in various combinations.…”

Section: Introductionmentioning

confidence: 99%

“…These glycoproteins are thus important targets for the immune system and have been experimentally used for immunization of animals against the homologous virus either individually or in various combinations. Direct injection of plasmid DNA expressing either EHV1-gB or gD has also been explored as a vaccine strategy (Fuentealba et al, 2019;Kukreja et al, 1998;Osterrieder et al, 1995;Ruitenberg et al, 1999;Weerasinghe et al, 2006;Wellington et al, 1996). In a recent study has shown that the use of live attenuated herpes simplex virus 1 vaccine strain VC2 expressing EHV-1 gD can efficiently infect equine cells and generate strong and protective anti EHV-1 immune response in mice (Liu et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Recombinant Glycoprotein B of Equine herpesvirus Type 1 Elicits Protective Immune Response against Challenge in BALB/c Mouse Model

Joshi

Gupta

Pavulraj

et al. 2021

IJAR

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Background: Equine herpesvirus type 1 (EHV-1) is the most important viral pathogen of equines, causing respiratory illness, abortion, neonatal foal mortality and neurologic disorders. Large numbers of commercial EHV-1 vaccines are available to protect equines from the disease, but they provide only partial protection. Despite immunization with inactivated and modified live virus vaccine, mares show abortions. Present study was aimed to investigate the immunogenicity and protective efficacy of EHV-1 recombinant glycoprotein B (rgB) and gB expressing plasmid DNA against EHV-1 infection in BALB/c mice model.Methods: About 3-4 weeks old 225 female BALB/c mice were selected for the comparative study of immunization followed by challenged with EHV-1/India/Tohana/96-2 strain virus in 5 different groups of 45 animals each.Result: Following immunization, rgB vaccinated mice showed optimal stimulation of EHV-1 gB specific cell mediated and humoral mediated immunity (HMI and CMI). The gB expressing plasmid DNA vaccinated mice developed only CMI while inactivated whole virus vaccinated mice had only HMI. Upon EHV-1 challenge, all infected mice displayed variable levels of clinical signs with changes in body weight, however, vaccinated mice showed very rapid recovery with optimal protection. Positive control group mice showed severe pulmonary lesions along with persistence virus infection till 5 days post challenge (dpc) whereas vaccinated mice had less pulmonary lesion only up to 3dpc. Minimal lung lesions and early virus clearance was observed in the rgB immunized mice in comparison to the gB plasmid DNA and inactivated EHV-1 vaccine immunized mice. It has been concluded that immunization with rgB elicits optimum protective immune response against EHV-1 infection in mice model. The rgB could be a potential vaccine candidate against EHV-1 infection in equine in the future.

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Immunoprotective response induced by recombinant glycoprotein D in the BALB/c respiratory mouse model of Equid alphaherpesvirus 1 infection

Cited by 2 publications

References 38 publications

Polystyrene nanoparticles induce stronger cellular and more durable humoral immune responses against an inactivated bovine herpesvirus 1 isolate

Polystyrene nanoparticles induce stronger cellular and more durable humoral immune responses against an inactivated bovine herpesvirus 1 isolate

Recombinant Glycoprotein B of Equine herpesvirus Type 1 Elicits Protective Immune Response against Challenge in BALB/c Mouse Model

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