“…The primary antibodies and corresponding TSA used for each protein were as follows: anti-CK (AE1/AE3, M3515, Dako, CA, USA) and Opal 520 for CK, anti-PD-L1 (E1L3N, #13684, Cell Signaling, Danvers, MA, USA) and Opal 540 for PD-L1, anti-CD20 (L26, ab133616, Leica, Wetzlar, Germany) and Opal 570 for CD20, anti-CD4 (EPR6855, NCL-L-CD20-L26, Abcam, Cambridge, MA, USA) and Opal 620 for CD4, anti-CD8 (4B11, NB100-65729, Novusbio, Centennial, CO, USA) and Opal 650 for CD8, and anti-FoxP3 (236A/E7, ab20034, Abcam, Cambridge, MA, USA) and Opal 690 for FoxP3. The methods have been introduced in detail in previous studies [32,33].…”