Immunoprevention of x-ray-induced leukemias in the C57BL mouse.

Peters, Robert L.; Sass, Bernard; Stephenson, John; Al-Ghazzouli, Ismail K.; Hino, Shinjiro; Donahoe, Robert M.; Kende, Meir; Aaronson, Stuart A.; Kelloff, Gary J.

doi:10.1073/pnas.74.4.1697

Cited by 23 publications

(7 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Passaged RadLV: The RadLV/RL1o is a thymotropic, highly leukemogenic virus reported to be isolated from a lymphoid cell line established from a primary X-ray-induced thymoma (10). None of our primary RadLVs were found to be as leukemogenic and strictly thymotropic as RadLV/RL1O, and the presence of a highly leukemogenic RadLV from primary thymomas has not been reported (9,11,12,17,18,30,33,40,46). RadLV/RL10 seems to be a unique virus which is biologically undistinguishable from the highly passaged BL/VL3 RadLVs.…”

Section: Discussionmentioning

confidence: 63%

“…On rare occasions, a weakly leukemogenic retrovirus could be recovered from these tumors (9,23,27), suggesting a role of this radiation leukemia virus (RadLV) in the etiology of the disease. But detection of such RadLV in primary thymomas was difficult and rare (9,11,12,17,18,30,33,40,46), and only the use of a more sensitive cocultivation assay has allowed us to detect the presence of ecotropic retroviruses frequently, within 3 months postirradiation (45). Moreover, we could frequently isolate a new class of gag-pol recombinant ecotropic, fibrotropic, weakly leukemogenic retroviruses in lymphoid cell lines established from primary thymomas (43), even though their proviruses were not readily detected in the primary thymoma (22).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Studies on emerging radiation leukemia virus variants in C57BL/Ka mice

Rassart¹,

Shang²,

Boie³

et al. 1986

J Virol

View full text Add to dashboard Cite

To analyze the emergence of radiation leukemia virus (RadLV) variants in primary X-ray-induced C57BL/Ka thymoma and to identify the virus responsible for the very high leukemogenic potential of passaged Kaplan strain BL/VL3 preparation, we cloned several primary and passaged ecotropic RadLV infectious genomes. By restriction analysis, we found that BL/VL3 cells harbor three related but different ecotropic RadLVs. Their restriction map differs significantly from those of primary RadLVs. Hybridization analysis also indicated that BL/VL3 and primary RadLVs differ in their pl5E and long terminal repeat (LTR) regions. As compared with the LTR sequence of the putative parental endogenous ecotropic provirus, the LTR sequence of primary weakly leukemogenic RadLV has only one change, a C-rich sequence, generating a 6-base-pair direct repeat just in front of the promotor. The LTR of the primary nonleukemogenic RadLV only showed few base changes, mainly clustered in R and U5. The LTR from a moderately leukemogenic passaged BLIVL3 RadLV had conserved the C-rich sequence and acquired a 43-base-pair direct repeat in U3 and several other point mutations, small insertions, and deletions scattered in U3, R, and U5. All cloned primary RadLVs were fibrotropic, and some were weakly leukemogenic. All cloned BL/VL3 RadLVs were thymotropic and nonfibrotropic. The block of their replication was found to be after the synthesis of unintegrated linear and supercoiled viral DNA. Most of the BL/VL3 RadLVs were moderately leukemogenic, and one (V-13) was highly leukemogenic, being as virulent as the Moloney strain. We propose a model for the emergence of the RadLV variants and show that the virus responsible for the high leukemogenic potential of BL/VL3 preparation is a nondefective, ecotropic, lymphotropic, nonfibrotropic, unique retrovirus which most likely arose from a parental primary RadLV similar to those studied here.

show abstract

Section: Discussionmentioning

confidence: 63%

mentioning

confidence: 99%

Studies on emerging radiation leukemia virus variants in C57BL/Ka mice

Rassart¹,

Shang²,

Boie³

et al. 1986

J Virol

View full text Add to dashboard Cite

show abstract

“…In the past, several investigators have reported the presence of leukemogenic viruses in cellfree extracts from primary X-ray-induced thymomas of C57BL/6 mice (13,15,28,34,47), but these wild-type viruses could not be fully characterized and propagated in vitro. The establishment of permanent tumor cell lines from our thymomas (42) has allowed us not only to further confirm earlier results on the presence of a leukemogenic MuLV in these tumors but also to determine that our lymphoid cell lines release a leukemogenic MuLV which is XC+, B-tropic, ecotropic, and fibrotropic.…”

Section: Discussionmentioning

confidence: 99%

New Class of Leukemogenic Ecotropic Recombinant Murine Leukemia Virus Isolated from Radiation-Induced Thymomas of C57BL/6 Mice

Rassart

Sankar-Mistry

Lemay

et al. 1983

J Virol

View full text Add to dashboard Cite

We previously reported the establishment of several lymphoid cell lines from Xray-induced thymomas of C57BL/Ka mice, and all, except one, produce retroviruses (P. Sankar-Mistry and P. Jolicoeur, J. Virol.35:270-275, 1980). Biological characterization offive of these new primary radiation leukemia viruses (RadLVs) indicated that they had a B-tropic, fibrotropic, and ecotropic host range and were leukemogenic when reinjected into C57BL/Ka newborn mice. The leukemogenic potential of one isolate (G6T2) was further assessed and shown to be retained after prolonged passaging on fibroblasts in vitro. Restriction endonuclease analysis of the DNA of four of our new RadLV isolates (G6T2, Ti-7, Ti-8, and Ti-9) revealed

show abstract

“…A weakly leukemogenic retrovirus has been isolated from these thymomas (5,15,19), and one such isolate was subsequently rendered highly potent by serial cell-free passages in vivo (13,19). Prevention of thymomas was achieved by active and passive immunization with MuLV antibodies (26), and leukemogenic MuLV has been isolated from several lymphoid cell lines established from these X-ray-induced tumors (18,29). Despite this, infectious MuLV or its footprints have been detected in only a low percentage of primary thymomas, and its presence appears rather elusive (5-7, 10, 11, 17, 19, 26, 31).…”

mentioning

confidence: 99%

Strong selection for cells containing new ecotropic recombinant murine leukemia virus provirus after propagation of C57BL/6 radiation-induced thymoma cells in vitro or in vivo.

Jolicoeur¹,

Rassart²,

Sankar-Mistry³

1983

Mol. Cell. Biol.

View full text Add to dashboard Cite

Using the Southern procedure, we have studied the presence of ecotropicspecific murine leukemia viral sequences in genomic DNA isolated from primary X-ray-induced thymomas, from lymphoid cell lines established from them, or from secondary tumors passaged in vivo. We found that primary radiation-induced thymomas and infiltrated spleens do not harbor newly acquired ecotropic provirus. However, additional ecotropic proviruses (which appear recombinant in the gagpol region) could be detected in most of the tumorigenic cell lines established in vitro from them and in tumors arising from subcutaneous transplantation of the primary thymomas. These results suggest that primary radiation-induced thymomas may not be clonal. They also indicate a strong correlation between the presence of ecotropic recombinant proviruses in the genome and the growth ability, both in vitro and in vivo, of specific cells within these thymomas, suggesting a possible mitogenic function for murine leukemia virus.

show abstract

Immunoprevention of x-ray-induced leukemias in the C57BL mouse.

Cited by 23 publications

References 21 publications

Studies on emerging radiation leukemia virus variants in C57BL/Ka mice

Studies on emerging radiation leukemia virus variants in C57BL/Ka mice

New Class of Leukemogenic Ecotropic Recombinant Murine Leukemia Virus Isolated from Radiation-Induced Thymomas of C57BL/6 Mice

Strong selection for cells containing new ecotropic recombinant murine leukemia virus provirus after propagation of C57BL/6 radiation-induced thymoma cells in vitro or in vivo.

Contact Info

Product

Resources

About