Immunoprecipitation and isoelectric focusing of sheep MHC class I antigens reveal higher complexity than serology

Abstract

Biochemical and serological methods were used to characterize sheep MHC class I polymorphism at the product level. The cells of 65 selected animals were subjected to immunoprecipitation and one-dimensional isoelectric focusing (IEF) with two cross-reacting monoclonal antibodies, B1.1G6 and HC-10. We were able to define up to 18 distinct haplotypes in the Latxa breed sample. Most of the locally defined serological specificities were confirmed or subdivided by biochemical typing. As IEF detected antigens not yet defined by serology, it provided us with a guide to producing more specific antisera by appropriate immunizations. Lastly, evidence of expression of two class I loci products was found.

Detection of polymorphisms in the tumour necrosis factor alpha candidate gene in sheep

“…We are interested in infectious diseases and, recently, TNF promoter polymorphisms have been associated with lack of control of HIV‐1 viremia (Delgado et al ., 2003). Our aim is also to investigate the relationship between the ovine TNF‐α gene and some other candidate genes located in the MHC region, such as MhcDRB1 and class I (Jugo & Vicario, 2000; Jugo et al ., 2002), and to try to differentiate their roles, as described by Ruuls & Sedgwick (1999).…”

Inbred or outbred? An evaluation of the functional allogenicity of farm sheep used in cardiac valve studies

“…Indeed, controversy remains regarding whether 2 or 3 loci encode ovine class I genes. 21,22 Inbreeding may reduce the number of MHC polymorphisms, thereby increasing the probability that foreign recipient tissue will be seen as self, thus attenuating the allogeneic immune response. 23 A variety of methods for MHC typing have been developed, including serologic testing 24 and DNA genotyping.…”