Immunopontentiating activities of the purified polysaccharide from evening primrose in H22 tumor-bearing mice

Zeng, Guang; Jin, Ying; Shen, Huan; Zhou, Nan; Huang, Li

doi:10.1016/j.ijbiomac.2012.10.005

Cited by 36 publications

(20 citation statements)

References 30 publications

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“…Blood ALT and AST are considered to be hepatic function parameters, and blood BUN, UA and CRE are considered to be renal function parameters [49]; these parameters were not significant different between the PTA group and the model group in our present study. These results indicate that PTA has no obvious systemic toxicity to major organs in H22 tumor-bearing mice.…”

Section: Discussioncontrasting

confidence: 43%

Anti-Tumor Effects of the Polysaccharide Isolated from Tarphochlamys Affinis in H22 Tumor-Bearing Mice

Tang

Huang

Xiong

et al. 2016

Cell Physiol Biochem

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Background: The previous studies have demonstrated that the polysaccharide isolated from Tarphochlamys affinis (PTA) exhibits anti-tumor effect on S₁₈₀ tumor-bearing mice and protective effects against hepatic injury. Methods: In this study, we investigated the anti-tumor activity and possible underlying mechanism of PTA on liver cancer using a murine H22 hepatocarcinoma model. Results: PTA was capable of repressing transplanted H22 solid hepatic tumor cell growth in vivo. The relative weight of immune organs (spleen and thymus) and lymphocyte proliferation induced by ConA or LPS were improved after PTA treatment. Furthermore, treatment with PTA promoted immune-stimulating serum cytokine secretion in H22 tumor-bearing mice. Additionally, the percentage of CD4⁺ T lymphocytes, CD8⁺ T lymphocytes and NK cells was increased in tumor-bearing mice following PTA administration. In tumor tissue, PTA significantly up-regulated the expression of Bax and p53 proteins and down-regulated the expression of Bcl-2 protein. In addition, at the therapeutic dose, PTA displayed very few toxic effects to major organs, such as the liver and kidney, in tumor-bearing mice. Conclusion: In H22 tumor-bearing mice, PTA exhibited prominent anti-tumor activity in vivo. The possible mechanism of action might be related to enhanced host immune system function and induction of H22 tumor cell apoptosis.

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Section: Discussioncontrasting

confidence: 43%

Anti-Tumor Effects of the Polysaccharide Isolated from Tarphochlamys Affinis in H22 Tumor-Bearing Mice

Tang

Huang

Xiong

et al. 2016

Cell Physiol Biochem

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“…In addition, CD4 T cells have been shown to express toll-like receptor-4 (TLR4), a receptor for LPS [ 28 , 29 ]. Stimulation of splenocyte viability ultimately increases the release of cytokines, potentially explaining the immune enhancement and antitumor activity [ 30 , 31 ]. Therefore, in our study, splenocytes were incubated with CY (1600 μ g/ml) or LPS ((10 μ g/ml), and ZPCO (0, 5, 10, 30, 50, 100, or 300 μ g/ml) for 24 h, after which cell viability was measured.…”

Section: Resultsmentioning

confidence: 99%

Immunostimulatory Effect of Zanthoxylum schinifolium‐Based Complex Oil Prepared by Supercritical Fluid Extraction in Splenocytes and Cyclophosphamide‐Induced Immunosuppressed Rats

Lee¹,

Park²,

Lee³

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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Complex oil of Zanthoxylum schinifolium and Perilla frutescens seed (ZPCO) is used as a traditional medicine due to its pharmacological activities. The aim of this study was to investigate the immunostimulatory effect of ZPCO in isolated splenocytes as well as in an immunosuppressed rat model, which was generated via oral administration of cyclophosphamide. Notably, our results showed that ZPCO exerted an immunity-enhancing effect both in vitro and in vivo. Specifically, ZPCO treatment enhanced the viability and inflammatory cytokine production of splenocytes and NK cell activity in vitro. Moreover, this product improved host defense under immunosuppressive conditions by increasing the number of immune cells and promoting the expression of cytokines involved in immune responses. Our results suggest that complex oil including Z. schinifolium should be explored as a novel immunostimulatory agent that could potentially be used for therapeutic purposes or as an ingredient in functional foods.

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“…In addition, an arabinoxylan isolated from rice bran increased CD4+ T cell function in human monocyte-derived dendritic cells [20], and increased T and B cell proliferation in both humans with and without cancer [13,27]. Previous studies have consistently reported that polysaccharides from evening primrose, Curcuma xanthorrhiza, and Angelica sinensis increase macrophage activity and/or T cell proliferation, either in vivo or in vitro [21,28,29]. These data, in combination with the data presented in this study, suggest that the increased production of IFN-γ resulting from RBEP supplementation may be mediated by either macrophages, B cells, or T cells, and it is probably not mediated by NK cells.…”

Section: Discussionmentioning

confidence: 99%

Dietary supplementation with rice bran fermented with Lentinus edodesincreases interferon-γ activity without causing adverse effects: a randomized, double-blind, placebo-controlled, parallel-group study

Choi

Paik

Kwon

et al. 2014

Nutr J

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BackgroundThe purpose of this study was to investigate the hypothesis that dietary supplementation with rice bran fermented with Lentinus edodes (rice bran exo-biopolymer, RBEP), a substance known to contain arabinoxylan, enhances natural killer (NK) cell activity and modulates cytokine production in healthy adults.MethodsThis study was designed in a randomized, double-blind, placebo-controlled, and parallel-group format. Eighty healthy participants with white blood cell counts of 4,000-8,000 cells/μL were randomly assigned to take six capsules per day of either 3 g RBEP or 3 g placebo for 8 weeks. Three participants in the placebo group were excluded after initiation of the protocol; no severe adverse effects from RBEP supplementation were reported. NK cell activity of peripheral blood mononuclear cells was measured using nonradioactive cytotoxicity assay kits and serum cytokine concentrations included interferon (IFN)-γ, tumor necrosis factor (TNF)-α, interleukin (IL)-2, IL-4, IL-10, and IL-12 were measured by Bio-Plex cytokine assay kit. This study was registered with the Clinical Research Information Service (KCT0000536).ResultsSupplementation of RBEP significantly increased IFN-γ production compared with the placebo group (P = 0.012). However, RBEP supplementation did not affect either NK cell activity or cytokine levels, including IL-2, IL-4, IL-10, IL-12, and TNF-α, compared with the placebo group.ConclusionsThe data obtained in this study indicate that RBEP supplementation increases IFN-γ secretion without causing significant adverse effects, and thus may be beneficial to healthy individuals. This new rice bran-derived product may therefore be potentially useful to include in the formulation of solid and liquid foods designed for treatment and prevention of pathological states associated with defective immune responses.

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Immunopontentiating activities of the purified polysaccharide from evening primrose in H22 tumor-bearing mice

Cited by 36 publications

References 30 publications

Anti-Tumor Effects of the Polysaccharide Isolated from Tarphochlamys Affinis in H22 Tumor-Bearing Mice

Anti-Tumor Effects of the Polysaccharide Isolated from Tarphochlamys Affinis in H22 Tumor-Bearing Mice

Immunostimulatory Effect of Zanthoxylum schinifolium‐Based Complex Oil Prepared by Supercritical Fluid Extraction in Splenocytes and Cyclophosphamide‐Induced Immunosuppressed Rats

Dietary supplementation with rice bran fermented with Lentinus edodesincreases interferon-γ activity without causing adverse effects: a randomized, double-blind, placebo-controlled, parallel-group study

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