Immunophenotyping of canine bronchoalveolar and peripheral blood lymphocytes

Dirscherl, Peter; Beisker, Wolfgang; Kremmer, Elisabeth; Mihalkov, A.; Voss, C.; Ziesenis, A.

doi:10.1016/0165-2427(94)05414-n

Cited by 17 publications

(12 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Although the percentages of CD4 ϩ and CD8 ϩ in PB are not always strictly comparable with results from other studies, 23,24 the CD4 : CD8 ratio was comparable. However, in the present study, significantly higher percentages of CD8 ϩ T cells were observed in the PB of the healthy randomsource dogs than in the PB of the purpose-bred Beagles.…”

Section: Discussioncontrasting

confidence: 44%

“…In the few previous reports of lymphocyte subpopulation analysis in the BALF from healthy dogs, the CD8 ϩ cell counts were higher than reported in the present study, which resulted in a lower CD4 : CD8 ratio. 24,25 In EBP patients, the numbers of CD4 ϩ and CD8 ϩ T cells in PB were significantly higher than in the control group. However, in the BALF of EBP dogs, there were significantly more CD4 ϩ T cells and significantly fewer CD8 ϩ T cells than in the control population.…”

Section: Discussionmentioning

confidence: 94%

See 1 more Smart Citation

An Immunologic Investigation of Canine Eosinophilic Bronchopneumopathy

et al. 2002

View full text Add to dashboard Cite

Immunologic variables in dogs with eosinophilic bronchopneumopathy (EBP) have not been extensively evaluated. The aim of this study was to determine immunoglobulin (Ig) concentrations and to perform phenotypic subtyping of lymphocytes in the bronchoalveolar lavage fluid (BALF) and peripheral blood (PB) of 12 dogs with EBP at the time of diagnosis (TD) and to compare these data with those obtained in healthy dogs, as well as in EBP dogs after antibiotic therapy (TAB) and during corticosteroid treatment (TM). Matched samples of serum and BALF were used to determine Ig concentrations (IgG, IgM, and IgA) by capture enzyme-linked immunosorbent assay (ELISA), from which a secretory index (SI) was calculated. Lymphocyte subpopulations were studied in the BALF and PB by flow cytometry. Log values of BALF IgM and IgA were significantly higher (0.64 Ϯ 0.05 and 1.06 Ϯ 0.13, respectively) in EBP dogs at TD than in controls and then tended to decrease at TM (0.55 Ϯ 0.03 and 1.02 Ϯ 0.17, respectively). A calculated SI for IgA was not significantly increased. In the BALF of dogs with EBP, the CD4 : CD8 was significantly (P Ͻ .05) higher (22.6 Ϯ 30.3) than in controls (3.2 Ϯ 1.9), due to significantly higher CD4 ϩ T cells and lower CD8 ϩ T cells. At TM, the BALF T-cell percentages returned to normal (2.4 Ϯ 0.6). We propose that the influx of eosinophils into the airway of dogs with EBP is at least in part mediated by cytokines derived from CD4 ϩ T cells. Further studies of canine cytokines and chemokines will help determine whether canine EBP involves type I hypersensitivity mechanisms regulated by Th2 lymphocytes.

show abstract

Section: Discussioncontrasting

confidence: 44%

Section: Discussionmentioning

confidence: 94%

An Immunologic Investigation of Canine Eosinophilic Bronchopneumopathy

et al. 2002

View full text Add to dashboard Cite

show abstract

“…The goals of this study were to identify changes in the peripheral blood lymphocyte distribution in dogs treated for lymphoma by use of well-characterized antibodies reactive with canine leukocytes. 7,8 A flow cytometric approach with approximately 1 mL of anticoagulated blood was readily adapted, and a reference range for aged clinically healthy dogs comparable to studies assessing a more limited range of markers in dogs 10,13,[17][18][19] and to values in the human literature 20 was established. Control dogs of similar age to dogs with lymphoma were recruited for this study because it has been suggested that with increasing age the proportion of CD4 ϩ and CD21 ϩ lymphocytes decreases, and the proportion of CD8 ϩ lymphocytes increases.…”

Section: Discussionmentioning

confidence: 99%

The Immunophenotype of Peripheral Blood Lymphocytes in Clinically Healthy Dogs and Dogs with Lymphoma in Remission

Gauthier

Aubert

Abrams‐Ogg

et al. 2005

Journal of Veterinary Internal Medicine

View full text Add to dashboard Cite

Lymphoma is a common cancer of dogs that frequently is treated with chemotherapy or radiation therapy. Response to therapy is variable and currently available diagnostic tests do not reliably predict response to therapy. Treatment for lymphoma often results in lymphopenia, but it is unknown whether the changes in circulating lymphocytes result from generalized or specific reduction of lymphocytes. In this study, blood lymphocytes from 12 clinically healthy dogs, 10 dogs in remission because of treatment for B-cell lymphoma, and 8 dogs in remission from T-cell lymphoma were analyzed by flow cytometry by using a panel of 20 antibodies reactive with canine leukocyte antigens. Results identified similar lymphocyte parameters in treated dogs regardless of the type of lymphoma. Treated dogs had Ͼ50% reduction in blood lymphocyte concentration, and an absolute decrease in most subsets of lymphocytes. Both groups of treated dogs had relative increases in the proportion of CD3 ϩ , T-cell receptor (TCR)␣␤ ϩ , and CD90ϩ lymphocytes, and a decreased proportion of CD45RA ϩ cells. In addition, dogs with T-cell lymphoma in remission had a significant increase in the proportion of CD49d ϩ lymphocytes. These findings were interpreted as representing likely suppression of lymphocyte regeneration by chemotherapy, with a relative increase in the proportion of memory over naïve lymphocytes. Lack of correlation with the T-or B-cell origin of the initial lymphoma suggested that, by using flow cytometric methods, residual circulating neoplastic cells could not be detected. However, the changes in the lymphocyte profile of dogs treated with chemotherapy may have relevance to their immunocompetence.

show abstract

“…24 We have shown here that in particular the CD4 T cells have a low CD45RA expression; the CD45RA bright CD4 T cells are less than 5% of the total CD4 T cells. This indicates that the CD4 T cells in the airway compartment have differentiated into the memory phenotype both in normal and allergic dogs.…”

Section: Discussionmentioning

confidence: 99%

Local T‐cell activation after segmental allergen challenge  in the lungs of allergic dogs

et al. 2002

View full text Add to dashboard Cite

SUMMARYDogs with immunoglobulin E (IgE) allergy for ragweed that are sensitized by intrapulmonary exposure to ragweed can be used to study the pulmonary immune response that is important in allergic asthma. Using this model, we tested the hypothesis that T lymphocytes are activated locally in the airways shortly after allergen exposure of the lungs. The airways of six allergic dogs and three non-allergic dogs were exposed to ragweed by segmental allergen challenge (SAC). T-cell subsets and T-cell activation in blood and bronchoalveolar lavage (BAL) fluid were measured by flow cytometry before SAC and at 4, 24 and 72 hr thereafter. SAC caused a statistically significant increase in the percentage of major histocompatibility complex (MHC) class II-positive CD4 and CD8 T cells in BAL fluid and a significant increase in the mean fluorescent activity of MHC class II from 4 hr after SAC onward. This activation was significantly different from that found in cells from lung lobes challenged with saline, or from lung lobes in non-allergic dogs challenged with ragweed. The percentage of CD45RA bright CD8 cells increased significantly in allergic dogs after both ragweed and saline challenges. This was significantly higher than in non-allergic dogs. We conclude that T-cell activation in the airways of dogs can be measured after in vivo activation of the cells by measuring MHC class II and CD45RA expression in BAL fluid T cells. Furthermore, in allergic dogs, T cells are activated locally in the lungs within 4 hr after exposure to ragweed allergen. These results suggest a role for T lymphocytes in the development of late-phase allergic reactions in the airways.

show abstract

Immunophenotyping of canine bronchoalveolar and peripheral blood lymphocytes

Cited by 17 publications

References 25 publications

An Immunologic Investigation of Canine Eosinophilic Bronchopneumopathy

An Immunologic Investigation of Canine Eosinophilic Bronchopneumopathy

The Immunophenotype of Peripheral Blood Lymphocytes in Clinically Healthy Dogs and Dogs with Lymphoma in Remission

Local T‐cell activation after segmental allergen challenge  in the lungs of allergic dogs

Contact Info

Product

Resources

About

Immunophenotyping of canine bronchoalveolar and peripheral blood lymphocytes

Cited by 17 publications

References 25 publications

An Immunologic Investigation of Canine Eosinophilic Bronchopneumopathy

An Immunologic Investigation of Canine Eosinophilic Bronchopneumopathy

The Immunophenotype of Peripheral Blood Lymphocytes in Clinically Healthy Dogs and Dogs with Lymphoma in Remission

Local T‐cell activation after segmental allergen challenge in the lungs of allergic dogs

Contact Info

Product

Resources

About

Local T‐cell activation after segmental allergen challenge  in the lungs of allergic dogs