Immunophenotyping of Acute Leukaemias. Immunophänotypisierung akuter Leukämien

Abstract

For nearly 100 years the classification of blood cells and the diagnosis of leukaemia have been based on cytomorphological features after staining. Even in the era of molecular biology this is still essential. Therapy of acute myeloid leukaemia (AML) is mostly dependent on the interpretation of the morphological appearance of blasts under the microscope. Cytomorphology should also lead to a rational use of techniques like immunophenotyping, cytogenetics, fluorescence / situ hybridisation (FISH), and polymerase chain reaction (PCR).In the past two decades, the impact of immunophenotyping by flow cytometry in the diagnosis and management of acute leukaemia has expanded rapidly. This has been mainly attributed to significant advances in laser and computer technologies and the production of several hundred monoclonal antibodies (moAbs) to a variety of antinens expressed by haematopoietic cells.This review concentrates on immunophenotyping of cells from patients with acute leukaemia and shows the clinical impact on diagnostics and treatment.

Immunophenotyic profile of adult acute lymphoblastic leukaemia in Iraq, a one year experience

“…It can provide correlated data that links different population profiles all based on single cell analysis. (4) In Iraq immunophenotyping has only been recently introduced to the panel of investigations for ALL. The aim of this study is to provide an overview of the immunophenotypic profile of adult ALL cases in Iraq and also study the frequency of expression of aberrant myeloid antigens in them.…”