Immunophenotyping lymphocyte and acute phase proteins in canine X-linked muscular dystrophy

Abstract

Duchenne Muscular Dystrophy (DMD) is the most common X-linked muscular disease affecting humans. The Golden Retriever Muscular Dystrophy model (GRMD) is consider the most suitable for several studies. This assay aims to quantify lymphocyte subpopulations CD4, CD5, and CD8, and standardize, the serum electrophoretic profile, to understand their contribution to the pathologic process in normal Golden Retriever dogs (GR group) and dystrophic´s (GRMD group), through the umbilical cord blood, in dogs aged from 2 to 3 months (GR II and GRMD II), and in dogs over 1 year of age (GR III and GRMD III). No significant differences were observed between the CD8+ lymphocyte subpopulations of the groups studied. The CD4+ and CD5+ lymphocyte subpopulations were significantly higher in the GRMD III group compared to the GR III group. Twenty-two different proteins in the gel were identified. The serum concentrations of the proteins belonging to the GR I and GRMD I groups were significantly lower than those of the other groups. We show that expression of acute phase proteins are worst during the aging of the dogs. We hope to expand knowledge to better understand the GRMD model and the translational data.

Analyses of the toxic properties of recombinant human Cyclophilin A in mice

“…Rapid increases in APP levels provide for early defense in an infectious process and precede a full immune response (Suffredini et al 1999). Evaluation of levels of APP levels during some diseases has been shown to be of prognostic significance during subsequent treatments (Abreu et al 2018;Ceron et al 2018). Data presented here indicated that rhCypA induced hepatic expression of the genes of several APP, and that the spectrum and level of expression of the APP genes, were directly dependent on rhCypA concentrations (see Figure 7).…”