Immunophenotypic Characterization of Normal Bone Marrow Stem Cells

Laranjeira, Pires; Ribeiro, Andreia; Mendes, Sandrine; Henriques, Ana; Pais, Maria Luísa; Paiva, Artur

doi:10.5772/38523

Cited by 3 publications

(5 citation statements)

References 42 publications

(47 reference statements)

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“…Their ability to differentiate into different tissues is variable according to their tissue of origin [ 4 ]. Bone marrow is the traditional source of human MSCs; however, there they represent a rare population of approximately 0.001% to 0.01% of total nucleated cells and their frequency tends to decline with increasing age [ 9 - 12 ]. Although adult MSCs have the ability to expand in culture while retaining their growth and multilineage potential [ 13 ], compared with MSCs from fetal sources, they undergo fewer cell divisions before they reach senescence [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Mesenchymal stem cells from umbilical cord matrix, adipose tissue and bone marrow exhibit different capability to suppress peripheral blood B, natural killer and T cells

Ribeiro¹,

Laranjeira²,

Mendes³

et al. 2013

Stem Cell Res Ther

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218

178

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IntroductionThe ability to self-renew, be easily expanded in vitro and differentiate into different mesenchymal tissues, render mesenchymal stem cells (MSCs) an attractive therapeutic method for degenerative diseases. The subsequent discovery of their immunosuppressive ability encouraged clinical trials in graft-versus-host disease and auto-immune diseases. Despite sharing several immunophenotypic characteristics and functional capabilities, the differences between MSCs arising from different tissues are still unclear and the published data are conflicting.MethodsHere, we evaluate the influence of human MSCs derived from umbilical cord matrix (UCM), bone marrow (BM) and adipose tissue (AT), co-cultured with phytohemagglutinin (PHA)-stimulated peripheral blood mononuclear cells (MNC), on T, B and natural killer (NK) cell activation; T and B cells’ ability to acquire lymphoblast characteristics; mRNA expression of interleukin-2 (IL-2), forkhead box P3 (FoxP3), T-bet and GATA binding protein 3 (GATA3), on purified T cells, and tumor necrosis factor-alpha (TNF-α), perforin and granzyme B on purified NK cells.ResultsMSCs derived from all three tissues were able to prevent CD4+ and CD8+ T cell activation and acquisition of lymphoblast characteristics and CD56dim NK cell activation, wherein AT-MSCs showed a stronger inhibitory effect. Moreover, AT-MSCs blocked the T cell activation process in an earlier phase than BM- or UCM-MSCs, yielding a greater proportion of T cells in the non-activated state. Concerning B cells and CD56bright NK cells, UCM-MSCs did not influence either their activation kinetics or PHA-induced lymphoblast characteristics, conversely to BM- and AT-MSCs which displayed an inhibitory effect. Besides, when co-cultured with PHA-stimulated MNC, MSCs seem to promote Treg and Th1 polarization, estimated by the increased expression of FoxP3 and T-bet mRNA within purified activated T cells, and to reduce TNF-α and perforin production by activated NK cells.ConclusionsOverall, UCM-, BM- and AT-derived MSCs hamper T cell, B cell and NK cell-mediated immune response by preventing their acquisition of lymphoblast characteristics, activation and changing the expression profile of proteins with an important role in immune function, except UCM-MSCs showed no inhibitory effect on B cells under these experimental conditions. Despite the similarities between the three types of MSCs evaluated, we detect important differences that should be taken into account when choosing the MSC source for research or therapeutic purposes.

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Section: Introductionmentioning

confidence: 99%

Mesenchymal stem cells from umbilical cord matrix, adipose tissue and bone marrow exhibit different capability to suppress peripheral blood B, natural killer and T cells

Ribeiro¹,

Laranjeira²,

Mendes³

et al. 2013

Stem Cell Res Ther

Self Cite

218

178

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“…Their number has been estimated at approximately 0.01% of nucleated BM cells and declines with age (36,37). Their number has been estimated at approximately 0.01% of nucleated BM cells and declines with age (36,37).…”

Section: Mesenchymal Stromal Cellsmentioning

confidence: 99%

“…However, MSC amplification in cultures produces changes in their phenotypic and genomic imprinting profiles and distorts their real distribution in comparison with in vivo models (36,37). However, MSC amplification in cultures produces changes in their phenotypic and genomic imprinting profiles and distorts their real distribution in comparison with in vivo models (36,37).…”

Section: Mesenchymal Stromal Cellsmentioning

confidence: 99%

“…The main obstacle for MSC isolation and characterization is their low frequency in fresh tissues, especially in BM. Their number has been estimated at approximately 0.01% of nucleated BM cells and declines with age (36,37).…”

Section: Mesenchymal Stromal Cellsmentioning

confidence: 99%

“…Frequently, sorting methods involving immunomagnetic beads or fluorescence-activated cell sorting (FACS) are used after an initial amplification of the MSCs in cultures. However, MSC amplification in cultures produces changes in their phenotypic and genomic imprinting profiles and distorts their real distribution in comparison with in vivo models (36,37). Ducret et al found an important diminution of CD271 + and STRO-1 + cells (<1%) in the culture expanded MSC populations compared with fresh human dental pulp (37).…”

Section: Mesenchymal Stromal Cellsmentioning

confidence: 99%

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Evaluation of bone marrow microenvironment could change how myelodysplastic syndromes are diagnosed and treated

Aanei

Catafal

2018

Cytometry Pt A

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Myelodysplastic syndromes are a heterogeneous group of clonal hematopoietic disorders. However, the therapies used against the hematopoietic stem cells clones have limited efficacy; they slow the evolution toward acute myeloid leukemia rather than stop clonal evolution and eradicate the disease. The progress made in recent years regarding the role of the bone marrow microenvironment in disease evolution may contribute to progress in this area. This review presents the recent updates on the role of the bone marrow microenvironment in myelodysplastic syndromes pathogenesis and tries to find answers regarding how this information could improve myelodysplastic syndromes diagnosis and therapy.

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Isolation and Characterization of Mesenchymal Stem Cells from Amniotic Membrane

Grãos¹,

Paiva²

2015

Amniotic Membrane

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Immunophenotypic Characterization of Normal Bone Marrow Stem Cells

Cited by 3 publications

References 42 publications

Mesenchymal stem cells from umbilical cord matrix, adipose tissue and bone marrow exhibit different capability to suppress peripheral blood B, natural killer and T cells

Mesenchymal stem cells from umbilical cord matrix, adipose tissue and bone marrow exhibit different capability to suppress peripheral blood B, natural killer and T cells

Evaluation of bone marrow microenvironment could change how myelodysplastic syndromes are diagnosed and treated

Isolation and Characterization of Mesenchymal Stem Cells from Amniotic Membrane

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