Immunophenotype and functional properties of feline dendritic cells derived from blood and bone marrow

Bienzle, Dorothee; Reggeti, F.; Clark, Mary E.; Chow, Catherine

doi:10.1016/s0165-2427(03)00132-6

Cited by 41 publications

(40 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In humans and other species, DC progenitor cells, such as CD14 ϩ monocytes, are present in peripheral blood and CD14 ϩ and/or adherent peripheral blood mononuclear cells (PBMCs) can be induced to differentiate in culture into monocyte-derived DCs in the presence of granulocytemacrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4) (1,16,29). Under these conditions, monocytes develop into immature DCs (iDCs).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Generation of Feline Dendritic Cells Derived from Peripheral Blood Monocytes for In Vivo Use

Freer

Matteucci

Mazzetti

et al. 2005

Clin Vaccine Immunol

View full text Add to dashboard Cite

Dendritic cells (DCs) are professional antigen-presenting cells that can prime T cells and polarize the cellular immune response. Because Th1-type immune responses have been connected to success in combating viral infection, a promising therapeutic application of DCs would be their differentiation in vitro and injection back into the host to boost an immune response in infected animals. This study was aimed both at developing a protocol to cultivate feline DCs in the absence of exogenous proteins for their use in vivo and at investigating what might be the most appropriate stimulus to induce their maturation in vitro and finding correlates of maturation. We generated DCs from peripheral blood monocytes in the presence of feline interleukin-4 and granulocyte-macrophage colony stimulating factor, and after 5 days their maturation was induced with either lipopolysaccharide, human recombinant tumor necrosis factor alpha, poly(I:C), or activated feline platelets. After 48 h, their CD14, CD1a, major histocompatibility complex class II, and B7.1 surface expression was analyzed in parallel with their ability to uptake antigen or prime a mixed leukocyte reaction. The results presented show that feline DCs cultured in autologous plasma differentiate and are able to mature in the presence of stimuli similar to the ones currently used for other species. The present work sets the grounds for future use of DCs obtained by the protocol described for in vivo vaccination and immunotherapy of feline immunodeficiency virus-infected cats.

show abstract

mentioning

confidence: 99%

“…On the other hand, very few studies have been performed so far on the characterization and culture conditions for feline DCs (1,4). Feline immunodeficiency virus infection in cats has long been studied as a model for human AIDS, but very little is known on the role of DCs in this particular context.…”

mentioning

confidence: 99%

Generation of Feline Dendritic Cells Derived from Peripheral Blood Monocytes for In Vivo Use

Freer

Matteucci

Mazzetti

et al. 2005

Clin Vaccine Immunol

View full text Add to dashboard Cite

show abstract

“…All cats were shown to be positive for FIV antibody and negative for feline leukemia virus Cell preparation: The methods for the preparation of DCs and macrophages were modified based on previously described protocols [4,13,27]. Briefly, heparinized whole blood was layered onto Ficoll/Hypaque (Lymphoprep; Nycomed Pharma AS, Oslo, Norway) and centrifuged at 560 g for 45 min at room temperature.…”

Section: Methodsmentioning

confidence: 99%

“…Monocytes incubated with granulocyte macrophage colony-stimulating factor (GM-CSF) and interleukin 4 (IL-4) acquire the properties of DCs. Moreover, several studies have been performed to establish a method for generating feline DCs [4,13,27]. Similar to human and murine DCs, feline DCs have been generated from monocytes cultured with GM-CSF and IL-4, and they also possessed the characteristic properties of DCs.…”

mentioning

confidence: 99%

Characterization of Monocyte-Derived Dendritic Cells from Cats Infected with Feline Immunodeficiency Virus

Mizukoshi

Baba

Horiuchi

et al. 2009

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

ABSTRACT. Dendritic cells (DCs) are professional antigen presenting cells (APCs) that possess an extraordinary capacity to stimulate naïve T cells and initiate a primary immune response. To develop a DC-based immunotherapy for feline immunodeficiency virus (FIV) infection, we carried out a study to characterize DCs from FIV-infected cats and compared the observations with those obtained from healthy controls. DCs were derived from adherent peripheral blood mononuclear cells that had been cultivated with recombinant feline interleukin 4, granulocyte macrophage colony-stimulating factor and heat-inactivated autologous plasma. Various parameters, such as cell morphology, surface phenotype, endocytosis and mixed leukocyte reaction (MLR), were analyzed to characterize feline DCs. Monocytederived DCs from FIV-infected cats as well as those from healthy controls showed a dendritic appearance and expressed an APC-like phenotype (CD1c + , CD80 + and MHC class II + ). However, the expression level of CD1a was variable in the DCs derived from FIVinfected cats, although this was not the case in the DCs derived from the healthy controls. DCs from the FIV-infected cats retained the ability to take up dextran via the mannose receptor and also showed an apparent MLR, indicating that these cells could be useful in immunotherapy. In this study, monocytes obtained from FIV-infected cats could differentiate into functional DCs, suggesting that they might be used in a DC-based immunotherapy against FIV infection.KEY WORDS: Dendritic cell, feline immunodeficiency virus, immunotherapy.

show abstract

“…Em animais domésticos, as DCs mieloides -derivadas de monócitos -foram caracterizadas em porcos (PAILLOT et al, 2001), vacas (HOWARD et al, 1999, ovelhas (CHAN et al, 2002), cavalos (HAMMOND et al, 1999), gatos (BIENZLE et al, 2003) e cães (GOODEL et al, 1985;DE CARVALHO et al, 2006). , 1986).…”

Section: "Your Time Is Limited So Don't Waste It Living Someone Elseunclassified

<b>Efeitos dos fatores tumorais derivados do melanoma canino na geração e maturação de células dendríticas caninas:</b> estudo <i>in vitro</i>

Silva¹

View full text Add to dashboard Cite

AGRADECIMENTOSAo homem que me mostrou como eu sou boa e como posso ser melhor, que me ama apesar do meu mau humor matinal, da loucura, da falta de tempo, do excesso de pêlos e de trabalho. Meu geofísico-mágico-programador-mecânico-amigo-namorado, muito obrigada. À Faculdade de Medicina Veterinária e Zootecnia da Universidade de SãoPaulo (FMVZ-USP). Minha eterna e acolhedora casa. Chegar todos os dias e sentir esse cheiro único é tranquilizador.

show abstract

Immunophenotype and functional properties of feline dendritic cells derived from blood and bone marrow

Cited by 41 publications

References 43 publications

Generation of Feline Dendritic Cells Derived from Peripheral Blood Monocytes for In Vivo Use

Generation of Feline Dendritic Cells Derived from Peripheral Blood Monocytes for In Vivo Use

Characterization of Monocyte-Derived Dendritic Cells from Cats Infected with Feline Immunodeficiency Virus

<b>Efeitos dos fatores tumorais derivados do melanoma canino na geração e maturação de células dendríticas caninas:</b> estudo <i>in vitro</i>

Contact Info

Product

Resources

About