Immunoperoxidase Localization of Apolipoprotein D in Human Enterocytes and Hepatocytes

Abstract

In this study we prepared a pure apolipoprotein D and obtained a specific antiserum to it. The purified apolipoprotein D migrated as a single band of Mr = 29,000 but appeared as five isoforms on isoelectrofocusing. The antiserum did not cross-react with other apolipoproteins. Immunoenzymatic staining revealed the presence of apolipoprotein D in the perinuclear area of the cytoplasm of isolated normal hepatocytes and HepG2 cells. Apolipoprotein D was also localized in intestinal epithelium and in liver cells. The intracellular distribution of apolipoprotein D was similar to that of apolipoprotein B. Our results indicated that apolipoprotein D, like many other circulating apolipoproteins, is synthesized in enterocytes and hepatocytes.

Apolipoprotein B epitopes are present in nuclear preparations of human and mouse cells

“…Notably, we detected ApoA-I, a major HDL constituent, in nuclear preparations of HDL-stimulated cells in accordance with previous reports suggesting that HDL-derived factors may well operate as nuclear factors (2,16). This idea is supported by recent evidence that the HDL atypical apolipoprotein ApoD can be detected within the nuclear space (17,18). Further experimental work will be necessary to confirm the pathobiological relevance of these findings.…”

The Lipocalin Apolipoprotein D Functional Portrait: A Systematic Review

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