Immunoneutralisation of GnRH-I, without cross-reactivity to GnRH-II, in the development of a highly specific anti-fertility vaccine for clinical and veterinary use

Ferro, Valerie A.; Khan, Md. Abu Hadi Noor Ali; Latimer, Valerie S; Brown, Donald E.; Urbanski, Henryk F.; Stimson, W. H.

doi:10.1016/s0165-0378(01)00071-7

Cited by 19 publications

(17 citation statements)

References 27 publications

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“…Likewise in our study, Ferro et al (16) indicated that the histological assessment showed drastic changes in testicular morphology, as well as atrophy to the seminiferous tubules and a total depression in spermatogenesis in the GnRH-I immunised animals. Marked atrophy of the testis leading to testicular failure was characterised by azoospermia of the seminiferous tubules.…”

Section: Discussionsupporting

confidence: 87%

“…The spermatogenic cells of the tubules appeared to become degenerated and were continuously cast off. The inner wall of the tubules contained only a small number of spermatogenic cells (spermatogonium and spermatocytes) and Sertoli cells, with no visible spermatozoa under light microscopy (16). Leydig cells lying in between the seminiferous tubules were virtually non-existent and rarely in small clusters, leaving only a few irregular polyhedral cells in rows together with blood vessels.…”

Section: Discussionmentioning

confidence: 98%

“…Ferro et al (16) found tubular folding and hyperplasia of tubule epithelia in all parts of the rat epididymis immunized with GnRH vaccine. Pseudo stratified columnar epithelia cells of ductuli efferentes changed into tall stratified columnar cells.…”

Section: Discussionmentioning

confidence: 99%

“…The most significant changes reported as a result of GnRH vaccine have been in the testis (9,10,16). Ferro et al (16) immunized rats with GnRH vaccine and observed testicular atrophy and azoospermia in seminiferous tubules by light microscope.…”

Section: Discussionmentioning

confidence: 99%

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Histological Investigation of Testicular and Accessory Sex Glands in Ram Lambs Immunized Against Recombinant GNRH Fusion Proteins

Odabaş

Kanter

2008

ELECTRON J GEN MED

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Aim:The aim of the study was to investigate the histological appearance of testis, epididymis, prostate and seminal vesicles of ram lambs immunized against gonadotrophin releasing hormone (GnRH).Methods: The study was performed on 14 male lambs, 7 in the study group and the other 7 in the control group. Another 5 lambs were included from the study after biopsy samples were taken from their testes at age 10 weeks to determine testicular histology at the start of the study. Seven male lambs aged 10 weeks were immunized against GnRH using a recombinant GnRH fusion protein vaccine. The immunization was repeated twice monthly until slaughter at 9 months of age. Testes and epididymis biopsies were taken at the 6th month. After slaughter, testis, epididymis, prostate and seminal vesicles were extracted for histopathological investigation.Results: At the 6th month and at slaughter histopathology of all the sex glands, most evidently in the testes, indicated secondary infertility. In the study group, tubule diameter decreased and spermatogenetic activity disappeared. The ball of sperm cells seen in the epididymis tubules of the control group at the 9th month was not seen in the study group. Changes in prostate tissues between the study and control groups were not evident but stromal tissue in the seminal vesicles increased in the study group.Conclusion: In conclusion, the histomorphological changes showed that recombinant GnRH fusion proteins provided immune castration in male lambs when used at an early age.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Histological Investigation of Testicular and Accessory Sex Glands in Ram Lambs Immunized Against Recombinant GNRH Fusion Proteins

Odabaş

Kanter

2008

ELECTRON J GEN MED

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“…These findings clearly have implications for the specificity and safety of GnRH-based vaccines. Studies are underway to develop vaccines which will have the desired effect of eliminating or reducing fertility but without causing pathology [39].…”

Section: Gonadotropin-releasing Hormone (Gnrh)mentioning

confidence: 99%

The development of contraceptive vaccines

Delves

2002

Expert Opinion on Investigational Drugs

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The use of vaccination as a means of controlling fertility was established during the last decade with the publication of a successful Phase II trial demonstrating the efficacy of this approach to family planning. However, only this one Phase II trial has been completed despite a plethora of hormonal and gamete antigens that have been proposed as candidate vaccines. Improvements in the design and formulation of contraceptive vaccines are underway and will be a necessary prelude to further clinical trials. AbstractThe use of vaccination as a means of controlling fertility was established during the last decade with the publication of a successful phase II trial demonstrating the efficacy of this approach to family planning. However, only this one phase II trial has been completed despite a plethora of hormonal and gamete antigens that have been proposed as candidate vaccines. Improvements in the design and formulation of contraceptive vaccines are underway and will be a necessary prelude to further clinical trials.

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Immune responses to a GnRH-based anti-fertility immunogen, induced by different adjuvants and subsequent effect on vaccine efficacy

Ferro

Costa

Carter

et al. 2004

Vaccine

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Immunoneutralisation of GnRH-I, without cross-reactivity to GnRH-II, in the development of a highly specific anti-fertility vaccine for clinical and veterinary use

Cited by 19 publications

References 27 publications

Histological Investigation of Testicular and Accessory Sex Glands in Ram Lambs Immunized Against Recombinant GNRH Fusion Proteins

Histological Investigation of Testicular and Accessory Sex Glands in Ram Lambs Immunized Against Recombinant GNRH Fusion Proteins

The development of contraceptive vaccines

Immune responses to a GnRH-based anti-fertility immunogen, induced by different adjuvants and subsequent effect on vaccine efficacy

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