Immunomorphometric variations of sustentacular cells of the male viscacha adrenal medulla during the annual reproductive cycle. Effects of androgens and melatonin

Gallol, Luis Ezequiel; Mohamed, Fabián

doi:10.1016/j.acthis.2018.03.009

Cited by 5 publications

(7 citation statements)

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“…In addition, our earlier findings indicated that PILE possesses anti-hyperglycemic activity ( 17 ). PILE mainly exerts this activity by four mechanisms: i) Acting as free radical scavengers in peroxidation and preventing the expression of oxidative stress-related proteins ( 12 , 18 , 30 ); ii) improving liver carbohydrate metabolism ( 31 ); iii) improving glucose uptake through mediators of the insulin signaling pathway ( 23 ); and iv) possessing α-glucosidase inhibitory effects, which can delay the breakdown of starch into glucose, increasing glucose uptake from the circulation, lowering blood glucose levels ( 32 ). Since a previous study reported that treatment with PILE preserved liver function by restoring AST, ALT and ALP levels in the mouse sera ( 17 ), the role of PILE treatment on hyperglycemia-induced liver damage was investigated in the present study.…”

Section: Discussionmentioning

confidence: 99%

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Treatment with Pluchea indica (L.) Less. leaf ethanol extract alleviates liver injury in multiple low‑dose streptozotocin‑induced diabetic BALB/c mice

2020

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Hyperglycemia-induced oxidative stress and inflammation are hallmarks of liver damage in diabetes mellitus. Accumulating evidence has demonstrated that Pluchea indica leaf ethanol extract (PILE) possesses strong antioxidant and anti-inflammatory properties. However, studies of its effects on liver damage in streptozotocin (STZ)-induced diabetic animals remain insufficient. To the best of our knowledge, the present study was the first to illustrate that PILE mitigated liver injury in STZ animals. Mice were first pretreated with PILE at either 50 mg/kg (PILE 50) or 100 mg/kg (PILE 100) 2 weeks prior to the induction of hyperglycemia by multiple low doses of STZ. The mice were then fed with PILE 50 or PILE 100 for 4 or 8 weeks, following which liver weight, pathological changes, oxidative stress parameters, inflammation-related markers and caspase-mediated apoptosis were measured at each time point. Untreated STZ mice exhibited abnormal increases in liver weight and severe pathological changes. However, PILE 100 reduced the severity of the STZ-induced diabetic phenotype at both time points. A significant decrease in the levels of superoxide dismutase and catalase, in addition to an increase in malondialdehyde, were observed in the livers of untreated STZ mice, all of which were significantly reversed by treatment with PILE 100 for 8 weeks. Western blot analysis revealed reduced levels of liver inflammatory markers, including interleukin-6, tumor necrosis factor-α, NF-κB p65, transforming growth factor-β1 and protein kinase C following PILE 100 treatment. Additionally, changes in the levels of apoptotic markers indicated that PILE 100 significantly attenuated caspase-9 and-3 expression, whilst preserving that of the Bcl-2 protein. In conclusion, the present study revealed that PILE alleviates hyperglycemia-induced liver injury by normalizing the various mediators of oxidative stress, inflammation and apoptosis.

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Section: Discussionmentioning

confidence: 99%

“…The threshold parameters remained consistent for all images. The % IA for each group was calculated by dividing the sum of the IA by the sum of the area of each microscopic field multiplied by 100 (23).…”

Section: Induction Of Diabetes In Experimental Animalsmentioning

confidence: 99%

Treatment with Pluchea indica (L.) Less. leaf ethanol extract alleviates liver injury in multiple low‑dose streptozotocin‑induced diabetic BALB/c mice

2020

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“…4,24 They are supposed to represent support elements, but also participate in the modulation of the activity of the adrenal medulla, probably through paracrine effects. 9 In human medicine a higher number of sustentacular cells in malignant pheochromocytomas has been described, 23 but not in others. 4,24 There are limitations to this study to be mentioned.…”

Section: Discussionmentioning

confidence: 99%

Pheochromocytoma in Dogs Undergoing Adrenalectomy

et al. 2018

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Pheochromocytoma is frequent in dogs and carries a guarded prognosis. Current histological criteria may not predict malignant behavior in dogs, similar to humans. In humans, characterization of tumors has been refined using the pheochromocytoma of the adrenal gland scaled score (PASS) and by immunohistochemistry. The study aim was to investigate PASS and immunohistochemical markers used in humans in 24 dogs with pheochromocytoma that underwent adrenalectomy. Dogs with pheochromocytomas were reviewed and tumors collected. Histological sections were evaluated to apply the PASS and were single-labeled for chromogranin A, Ki-67, COX-2, p53, BCL-2, c-erbB-2, vascular endothelial growth factor, and S100. Survival, age, and vascular and capsular invasion were compared for PASS and immunohistochemical markers; results of PASS were also compared for each marker. Associations between markers were tested. PASS and immunohistochemical markers did not differ for survival, age, and vascular and capsular invasion. Tumors showing BCL-2 expression in >50% cells had lower PASS than those with lower expression (PASS: 7 ± 2 vs 9 ± 2; P = .011). Tumors positive for S100 had higher PASS than those that were negative (PASS: 10 ± 2 vs 7 ± 2; P = .001). Results of the different markers were not associated. In conclusion, in the context of canine pheochromocytoma, PASS and the selected immunohistochemical markers are not associated with survival, age, or vascular or capsular invasion. The higher PASS in S100-positive tumors may indicate that pheochromocytomas developing morphologic changes acquire S100 expression. The significance of lower PASS in tumors with elevated BCL-2 expression is uncertain. Overall, the use of PASS and the present immunohistochemical markers may not be useful in dogs with pheochromocytoma.

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“…After 9 weeks of melatonin administration, all animals were sacrificed and the adrenal glands were excised. This experimental design has already been used in previous experiments for this species (Gallol & Mohamed, 2018; Rosales, Busolini, Mohamed, & Filippa, 2016).…”

Section: Methodsmentioning

confidence: 99%

“…After 6 weeks, all animals were sacrificed and the adrenal glands were excised. This time length has been used in previous castration experiments conducted in this species (Filippa, Godoy, Perez, & Mohamed, 2014; Gallol & Mohamed, 2018), and it is similar to the castration time length in which major histological changes could be observed in the adrenal gland of other rodents (Benmouloud et al, 2014; Hall & Korenchevsky, 1938). Gonadal activity in the control group was assessed through histological examination of testes.…”

Section: Methodsmentioning

confidence: 99%

Influence of melatonin and sexual hormones on the expression of proliferating cell nuclear antigen in the adrenal cortex of a seasonal breeder (Lagostomus maximus)

Gallol

Busolini

Mohamed

2020

The Anatomical Record

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The viscacha (Lagostomus maximus) is a rodent of nocturnal habits, whose physiology and behavior vary according to modifications of environmental signals. The objective of this study is to assess the influence of melatonin and sexual hormones on the viscacha adrenal cortex proliferative activity through the immunohistochemical detection of proliferating cell nuclear antigen (PCNA) along with hormonal determinations. PCNA expression was studied in male viscachas to assess the effect of melatonin administration, castration, and the annual reproductive cycle. In female viscachas, PCNA was studied in nonpregnant and pregnant viscachas. PCNA expression was observed in adrenocortical cells (PCNA-A) and endothelial cells (PCNA-E). Melatonin-administered animals showed a significantly lower number of PCNA-A compared to the control group. No significant difference could be established in the number of PCNA-A and PCNA-E between castrated and control animals. However, the morphometric analysis showed an increase in the size of the cortex of castrated animals, along with other cytological features. Significant differences in serum testosterone levels were observed during the male viscacha reproductive cycle, with the lowest levels encountered during the regression period (winter). Male viscachas exhibited a significantly high number of PCNA-A during late autumn and a high number of PCNA-E during winter. In females, hormonal determinations showed a peak of progesterone and estrogen during mid-pregnancy, along with a notably high number of PCNA-A and an increase in the number of PCNA-E. Our results suggest that proliferation in the adrenal cortex of the viscacha varies in relation to melatonin, sexual hormones, and environmental conditions.

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Immunomorphometric variations of sustentacular cells of the male viscacha adrenal medulla during the annual reproductive cycle. Effects of androgens and melatonin

Cited by 5 publications

References 59 publications

Treatment with Pluchea indica (L.) Less. leaf ethanol extract alleviates liver injury in multiple low‑dose streptozotocin‑induced diabetic BALB/c mice

Treatment with Pluchea indica (L.) Less. leaf ethanol extract alleviates liver injury in multiple low‑dose streptozotocin‑induced diabetic BALB/c mice

Pheochromocytoma in Dogs Undergoing Adrenalectomy

Influence of melatonin and sexual hormones on the expression of proliferating cell nuclear antigen in the adrenal cortex of a seasonal breeder (Lagostomus maximus)

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