Immunomodulatory Effects of the Water-soluble Lectin from Moringa oleifera Seeds (WSMoL) on Human Peripheral Blood Mononuclear Cells (PBMC)

Coriolano, Marília Cavalcanti; Brito, Jéssica de Santana; Patriota, Leydianne Leite de Siqueira; Soares, Ana Karine de Araújo; Lorena, Virgínia Maria Barros de; Paiva, Patrícia Maria Guedes; Napoleão, Thiago Henrique; Melo, Cristiane Moutinho Lagos de

doi:10.2174/0929866525666180130141736

Cited by 27 publications

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“…Bioactive compounds identified in the plants include; for V. amygdalina - vernolide (116), vernodalin (117), hydroxyvernolide (120) and vernodalol (123), vernoniosides B1-B3 and vernoniosides A1-A4 (124) ; for N. pobeguinii - strictosamide (138), 19-O-methylangustoline, angustoline (139) , A. Mexicana - berberine (140), tetrahydroberberine, protopine (141), benzophenanthridines, 8-acetonyl dihydrosanguiranine, 8-methoxy dihydrosanguiranine (142) , pancorine (144) , O-methylzanthoxyline (145) , nor-chelerythrine (125) , arnottianamide (146) cryptopine (147) , muramine (148) , argemexicaine A, argemexicaine B (149) ; for A. annua - artemisinin (157) ; C. aurantiifolia - apigenin (156) and Morinda lucida - Morindin (154), oruwal (152), oruwalol (155), oruwacin (150), molucidin (151), Damnacanthal (153), Ursolic acid (17), polypeptides ( Kraft et al, 2003 ; Challand and Willcox, 2009 ; Brahmachari et al, 2013 ; Haidara et al, 2016 ; Haudecoeur et al, 2018 ; Divneet Kaur, 2019 ). Overwhelming evidence supports the standardization of the leaf and seed of M. oleifera for a possible clinical application [ Level III ] as it has demonstrated broad range of antiviral activity in various studies ( Biswas D. et al, 2020 ) while the disulphide-stabilized miniproteins (Morintides), lectins, hevein-like peptides, protein hydrolysates and glucosinolates/isothiocynates isolated from the plant have shown impressive effects, including as antiadhesives, anti-inflammatory, antioxidants and immunomodulatory compounds ( Kini et al, 2017 ; Moura et al, 2017 ; Coriolano et al, 2018 ; Fahey et al, 2019 ; Liang et al, 2019 ; Sousa et al, 2020 ). Aside immunomodulation and free radical scavenging, one mechanism of activity of these lectins and stable polypeptides involve the competitive inhibition of adhesion of pathogen proteins to host polysaccharide receptors [ Level III, V ] ( Sharon, 1986 ; Boas et al, 2019 ).…”

Section: Methodsmentioning

confidence: 99%

Therapeutic Potentials of Antiviral Plants Used in Traditional African Medicine With COVID-19 in Focus: A Nigerian Perspective

et al. 2021

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The coronavirus disease 2019 (COVID-19) pandemic is caused by an infectious novel strain of coronavirus known as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) which was earlier referred to as 2019-nCoV. The respiratory disease is the most consequential global public health crisis of the 21st century whose level of negative impact increasingly experienced globally has not been recorded since World War II. Up till now, there has been no specific globally authorized antiviral drug, vaccines, supplement or herbal remedy available for the treatment of this lethal disease except preventive measures, supportive care and non-specific treatment options adopted in different countries via divergent approaches to halt the pandemic. However, many of these interventions have been documented to show some level of success particularly the Traditional Chinese Medicine while there is paucity of well reported studies on the impact of the widely embraced Traditional African Medicines (TAM) adopted so far for the prevention, management and treatment of COVID-19. We carried out a detailed review of publicly available data, information and claims on the potentials of indigenous plants used in Sub-Saharan Africa as antiviral remedies with potentials for the prevention and management of COVID-19. In this review, we have provided a holistic report on evidence-based antiviral and promising anti-SARS-CoV-2 properties of African medicinal plants based on in silico evidence, in vitro assays and in vivo experiments alongside the available data on their mechanistic pharmacology. In addition, we have unveiled knowledge gaps, provided an update on the effort of African Scientific community toward demystifying the dreadful SARS-CoV-2 micro-enemy of man and have documented popular anti-COVID-19 herbal claims emanating from the continent for the management of COVID-19 while the risk potentials of herb-drug interaction of antiviral phytomedicines when used in combination with orthodox drugs have also been highlighted. This review exercise may lend enough credence to the potential value of African medicinal plants as possible leads in anti-COVID-19 drug discovery through research and development.

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Section: Methodsmentioning

confidence: 99%

Therapeutic Potentials of Antiviral Plants Used in Traditional African Medicine With COVID-19 in Focus: A Nigerian Perspective

et al. 2021

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“…Plant lectins can induce the expression of humoral effectors against tumors or microbial infections [136,[141][142][143]. Activated macrophages can produce cytokine signal molecules.…”

Section: Plant Lectins Promote the Release Of Cytokines And Other Effectorsmentioning

confidence: 99%

“…The lectins stimulate cytokine production in different types of immune-related mammalian cells, such as murine macrophages, spleen cells, dendritic cells, mouse spleen cells, and human dendritic cells, neutrophils, peripheral blood mononuclear cells, etc. [136,142,143].…”

Section: Plant Lectins Promote the Release Of Cytokines And Other Effectorsmentioning

confidence: 99%

Can Plant Lectins Help to Elucidate Insect Lectin-Mediated Immune Response?

Chen

Schutter

Damme

et al. 2021

Insects

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Lectins are carbohydrate-binding proteins that recognize and selectively bind to specific sugar structures. This group of proteins is widespread in plants, animals, and microorganisms, and exerts a broad range of functions. Many plant lectins were identified as exogenous stimuli of vertebrate immunity. Despite being the largest and most diverse taxon on earth, the study of lectins and their functions in insects is lagging behind. In insects, research on lectins and their biological importance has mainly focused on the C-type lectin (CTL) family, limiting our global understanding of the function of insect lectins and their role in insect immunity. In contrast, plant lectins have been well characterized and the immunomodulatory effects of several plant lectins have been documented extensively in vertebrates. This information could complement the missing knowledge on endogenous insect lectins and contribute to understanding of the processes and mechanisms by which lectins participate in insect immunity. This review summarizes existing studies of immune responses stimulated by endogenous or exogenous lectins. Understanding how lectins modulate insect immune responses can provide insight which, in turn, can help to elaborate novel ideas applicable for the protection of beneficial insects and the development of novel pest control strategies.

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“…[8][9][10] A WSMoL demonstrou atividade anti-inflamatória in vitro em macrófagos murinos estimulados por lipopolissacarídeos, 11 e foi capaz de ativar linfócitos humanos a partir de culturas de células mononucleares do sangue periférico, mostrando um efeito imunomodulador. 12 Também tem sido comprovado que WSMoL é uma das proteínas coagulantes encontradas nas sementes de M. oleifera, 8,13 e é capaz de reduzir a turbidez e a ecotoxicidade de amostras de água coletadas de um riacho poluído. 14 Está bem demonstrado que muitos antibióticos e algumas classes de drogas anti-inflamatórias estão frequentemente associados a efeitos cardiotóxicos.…”

Section: Introductionunclassified

Avaliação dos Efeitos Cardíacos de Lectina Solúvel em Água (WSMoL) de Sementes de Moringa Oleifera

et al. 2020

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ResumoFundsamento: As sementes de Moringa oleifera, que são utilizadas para clarificação de água, contêm uma lectina chamada WSMoL que tem mostrado atividade antibacteriana e imunomoduladora in vitro. Devido ao seu valor nutritivo e potencial terapêutico, as folhas e as sementes dessa árvore são consumidas em algumas comunidades. Algumas lectinas de plantas não são tóxicas para mamíferos, mas tem sido relatado que outras são prejudiciais quando ingeridas ou administradas por outros meios.Objetivo: Como um dos passos necessários para determinar a segurança de WSMoL, nós avaliamos os possíveis efeitos cardiotóxicos desta proteína purificada.Métodos: Durante 21 dias consecutivos, a WSMoL foi administrada a camundongos por gavagem. Foram investigadas as funções eletrofisiológicas, mecânicas e metabólicas in vivo e ex vivo por meio de registros eletrocardiográficos, ressonância magnética nuclear e respirometria de alta resolução. Resultados: O tratamento com WSMoL não induziu alterações nos níveis de glicose no sangue ou peso corporal emcomparação com o grupo controle. Adicionalmente, as relações peso cardíaco/peso corporal e peso cardíaco/comprimento tibial estavam semelhantes em ambos os grupos. A ingestão de lectina também não modificou a tolerância à glicose ou resistência à insulina. Não foram observadas alterações nos parâmetros eletrocardiográficos ou na duração do potencial de ação cardíaco. Os corações dos camundongos dos grupos controle e WSMoL mostraram função ventricular esquerda preservada. Além disso, a WSMoL não induziu alterações na função mitocondrial (em todos os casos, p > 0,05). Conclusões: A administração de WSMoL demonstrou ter um perfil de segurança cardíaca. Estes resultados contribuem à avaliação de segurança do uso de sementes de M. oleifera para tratar água, visto que essa lectina está presente na preparação empregada por algumas populações com esse fim. (Arq Bras Cardiol. 2020; [online].ahead print, PP.0-0) Palavras-chave: Moringa Oleifera; Lectinas; Glicosídeos; Carboidratos; Coração; Segurança Hídrica; Camundongos. AbstractBackground: Moringa oleifera seeds, which are used for water clarification, contain a lectin named WSMoL which has shown in vitro antibacterial and immunomodulatory activity. Due to their nutritional value and therapeutic potential, the leaves and seeds of this tree are eaten in some communities. Some plant lectins are non-toxic to mammals, but others have been reported to be harmful when ingested or administered by other means.Objective: As one of the steps needed to define the safety of WSMoL, we evaluated possible cardiotoxic effects of this purified protein.Methods: WSMoL was administered for 21 consecutive days to mice by gavage. Electrophysiological, mechanical, and metabolic cardiac functions were investigated by in vivo and ex vivo electrocardiographic recordings, nuclear magnetic resonance, and high-resolution respirometry. Results:The treatment with WSMoL did not induce changes in blood glucose levels or body weight in comparison with control group. Moreover, ...

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Immunomodulatory Effects of the Water-soluble Lectin from Moringa oleifera Seeds (WSMoL) on Human Peripheral Blood Mononuclear Cells (PBMC)

Abstract: WSMoL promotes immunomodulation in human PBMC inducing a potential wound healing profile and, in future in vivo assays, can be evaluated as adjuvant in immunosuppressive diseases and wound repair.

Cited by 27 publications

References 0 publications

Therapeutic Potentials of Antiviral Plants Used in Traditional African Medicine With COVID-19 in Focus: A Nigerian Perspective

Therapeutic Potentials of Antiviral Plants Used in Traditional African Medicine With COVID-19 in Focus: A Nigerian Perspective

Can Plant Lectins Help to Elucidate Insect Lectin-Mediated Immune Response?

Avaliação dos Efeitos Cardíacos de Lectina Solúvel em Água (WSMoL) de Sementes de Moringa Oleifera

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