Immunomodulatory effects of the polysaccharide from <i>Sinonovacula constricta</i> on RAW264.7 macrophage cells

Liu, Zhi-Dong; Liu, Zhifang; Li, Laihao; Zhang, Junjie; Zhao, Qiancheng; Lin, Na; Zhong, Wenzhu; Jiang, Mei

doi:10.1002/fsn3.2735

Cited by 15 publications

(6 citation statements)

References 48 publications

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“…Figure 6 showed that transcription levels of all three were significantly increased within the limits of SRP (25-50 μg/mL) or LPS (1 μg/mL) groups compared with the blank group ( p <0.05). The result was similar to the results reported by Liu on the immunomodulatory activity of Sinonovacula constricta polysaccharide ( 32 ).…”

Section: Results and Analysissupporting

confidence: 91%

Three-phase extraction of polysaccharide from Stropharia rugosoannulata: Process optimization, structural characterization and bioactivities

Zhang

Wang

et al. 2023

Front. Immunol.

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The isolation of Stropharia rugosoannulata polysaccharide (SRP) by three-phase extraction was optimized, and its structure and biological activities were identified. The optimal extraction conditions were: mass fraction of ammonium sulfate, 20%; volume ratio of sample solution to t-butanol, 1:1.5; extraction temperature, 35°C. Under these conditions, the yield of SRP was 6.85% ± 0.13%. SRP was found to be composed of glucose (35.79%), galactose (26.80%), glucuronic acid (9.92%), fructose (8.65%), xylose (7.92%), fucose (4.19%), arabinose (3.46%) and rhamnose (3.26%), with the molecular weight of 27.52 kDa. The results of DPPH, hydroxyl, ABTS+ radical scavenging and reducing power tests showed that SRP had good antioxidant capacities. SRP had no cytotoxic effect on RAW264.7 macrophages at the concentrations of 25-200 μg/mL, and could significantly promote phagocytosis activity and cell migration according to CCK-8 assay, phagocytosis assay and cell scratch experiment. SRP can significantly stimulate the transcript expression levels of TNF-α, IL-1β and IL-6, as determined by RT-PCR and Western blot assays. SRP activated the TLR4/NF-κB signaling pathway, and autophagy also occurred. These results suggest that SRP is a safe antioxidant and immunomodulator, and that it can be used in the development of functional foods and/or pharmaceuticals.

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Section: Results and Analysissupporting

confidence: 91%

Three-phase extraction of polysaccharide from Stropharia rugosoannulata: Process optimization, structural characterization and bioactivities

Zhang

Wang

et al. 2023

Front. Immunol.

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“…Besides NO, activated RAW264.7 cells could also motivate the secretion of all kinds of immune cytokines, including IL-1β, IL-6 and TNF-α, which is important to body's inflammation and immunomodulatory. [44] After treated with GP1, the levels of IL-1β increased with concentration dependently, and increased by 52.37% at 5000 μg/mL (Figure 4F). Moreover, IL-6 increased by 89.13%, 90.20% and 96.03% at 5, 200 and 5000 μg/mL, respectively (Figure 4G).…”

Section: Antioxidant Activities Of Gp1mentioning

confidence: 94%

Structure of a new low molecular weight Glycyrrhiza polysaccharide and improvement on immune disorders

Zhang,

Song,

Shi

et al. 2024

Preprint

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Polysaccharides from Glycyrrhiza are known to have several bioactive effects. Current studies on polysaccharides mainly focused on the biological activity of mixed components or large molecular weight polysaccharides. However, the structure-activity relationship between polysaccharides and immune regulation is still unclear. In this work, a low molecular weight GP1 (6.5 kDa) mainly composed of glucose was purified. Its backbone was determined as →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→ (9.3%), →4,6)-α-D-Glcp-(1→ and α-D-Glcp-(1→ residues. This structure gives GP1 the good antioxidant ability (91.85%) and phagocytic activity (129.15%) of RAW264.7 cell. Through in-vitro simulation, it was found that GP1 could be degraded by intestinal flora, increase the production of SCFAs by 83.44%, and promote the proliferation of beneficial bacteria. Further studies showed that GP1 effectively increased body weight, immune organ index, and intestinal barrier function of immunosuppressed mice. And GP1 also promoted the proliferation of goblet cells, CD4+ and CD8+ T lymphocytes by 31.40%, 20.61% and 50.15%, respectively. Meanwhile, GP1 also activated the expression ofTLR2, TLR4 and TLR6 gene in the TLR signaling pathway on the immune cell surface, thereby increasing the release of cytokines. These results indicated that the immunomodulatory of GP1 was closely related to intestinal flora, immune cell and TLR genes up-regulation.

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“…The membranes were subsequently blocked in TBST solution containing 5% skimmed milk at room temperature for 2 h and incubated with corresponding primary antibodies overnight at 4 °C, followed by incubating with corresponding secondary antibody at 37 °C for 3 h. The proteins were detected using a gel imaging system (Yuehua, Guangdong, China) using enhanced chemiluminescence. The protein band intensities were determined via ImageJ software, and the relative expression levels of Keap1, Nrf2 and HO‐1 were calculated by normalising with levels of β actin (Jia et al ., 2021; Liu et al ., 2022).…”

Section: Methodsmentioning

confidence: 99%

The structural characteristic of bamboo shoot shell polysaccharides extracted using ultrasound‐assisted phosphotungstic acid hydrolysis and its protection against cell oxidative injury

Dai

Xiao

et al. 2022

Int J of Food Sci Tech

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Summary In this study, crude polysaccharide (BSSP) was extracted from bamboo (Phyllostachys edulis) shoot shell using the ultrasonic‐assisted phosphotungstic acid hydrolysis method. Then, BSSP was further fractionated by DEAE Sepharose fast flow column and serial NaCl solution elutions to obtain two fractions, BSSP‐A and BSSP‐B. The results showed that the maximum yield of BSSP was 195.2 mg g−1 (19.52%) under optimised conditions. Structural analysis indicated that both BSSP‐A and BSSP‐B were heteropolysaccharides with α‐pyranoside bond structure and mainly consisted of xylose by scanning electronic microscopy, Fourier transform infrared spectroscopy, ultraviolet–visible spectroscopy, thermal characterisation and nuclear magnetic resonance spectroscopy. BSSP‐B showed a better protective effect than BSSP‐A against H2O2‐induced oxidative injury in WRL‐68 cells by protecting the cell membrane structure, maintaining mitochondrial membrane potential and increasing intracellular antioxidant enzymes activities. Immunoblotting indicated that polysaccharides might up‐regulate the expression of antioxidant enzymes through the Keap1/Nrf2 signalling pathway and thus alleviate H2O2‐induced cell injury. The findings of this study suggested that polysaccharides from bamboo shoot shell may act as potent antioxidants in functional food industries.

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Immunomodulatory effects of the polysaccharide from Sinonovacula constricta on RAW264.7 macrophage cells

Cited by 15 publications

References 48 publications

Three-phase extraction of polysaccharide from Stropharia rugosoannulata: Process optimization, structural characterization and bioactivities

Three-phase extraction of polysaccharide from Stropharia rugosoannulata: Process optimization, structural characterization and bioactivities

Structure of a new low molecular weight Glycyrrhiza polysaccharide and improvement on immune disorders

The structural characteristic of bamboo shoot shell polysaccharides extracted using ultrasound‐assisted phosphotungstic acid hydrolysis and its protection against cell oxidative injury

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