Immunomodulatory effects of seed peptide lunasin in RAW264.7 macrophages in obese microenvironments

Huang, Peiying; Hsieh, Chia-Chien

doi:10.1016/j.jff.2023.105719

Cited by 4 publications

(3 citation statements)

References 45 publications

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“…Compared with the control group, OVAH significantly enhanced the phagocytosis rate of the RAW 264.7 cells with the highest phagocytosis rate (186.25 ± 10.00%) at a concentration of 100 μg/mL ( P < 0.05). This result was higher than that reported in soybean peptides and lunasin seed peptides . OVAH concentrations >100 μg/mL significantly reduced the cell phagocytosis rate ( P < 0.05).…”

Section: Resultssupporting

confidence: 92%

“…This result was higher than that reported in soybean peptides 31 and lunasin seed peptides. 32 OVAH concentrations >100 μg/mL significantly reduced the cell phagocytosis rate (P < 0.05). Guo et al 33 suggested that low concentrations of polyphenols (50 μg/mL) enhanced immune responses, while polyphenols at high concentrations (800 μg/mL) inhibited inflammation development in RAW 264.7 macrophages, which is similar to our results.…”

Section: Phagocytic Capacitymentioning

confidence: 94%

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Identification and Molecular Mechanism of Novel Two-Way Immunomodulatory Peptides from Ovalbumin: In Vitro Cell Experiments, De Novo Sequencing, and Molecular Docking

Li,

Abou-Elsoud,

Chen

et al. 2024

J. Agric. Food Chem.

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The purpose of this study was to identify ovalbumin-derived immunomodulatory peptides by in vitro cell experiments, de novo sequencing, and molecular docking. Ovalbumin hydrolysates were prepared by two enzymes (alkaline protease and papain) individually, sequentially, or simultaneously, respectively. The simultaneous enzymatic hydrolysate (OVAH) had a high degree of hydrolysis (38.12 ± 0.48%) and exhibited immune-enhancing and anti-inflammatory activities. A total of 160 peptides were identified by LC−MS/MS in OVAH. Three novel peptides NVMEERKIK, ADQARELINS, and WEKAFKDE bound to TLR4-MD2 through hydrogen bonds and hydrophobic interactions with high binding affinity and binding energies of −181.40, −178.03, and −168.12 kcal/mol, respectively. These three peptides were synthesized and validated for two-way immunomodulatory activity. NVMEERKIK exhibiting the strongest immunomodulatory activity, increased NO and TNF-α levels by 128.69 and 38.01%, respectively, in normal RAW264.7 cells and reduced NO and TNF-α levels by 27.31 and 39.13%, respectively, in lipopolysaccharideinduced inflammatory RAW264.7 cells. Overall, this study first revealed that ovalbumin could be used as an immunomodulatory source for controlling inflammatory factor secretion.

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Section: Resultssupporting

confidence: 92%

Section: Phagocytic Capacitymentioning

confidence: 94%

Identification and Molecular Mechanism of Novel Two-Way Immunomodulatory Peptides from Ovalbumin: In Vitro Cell Experiments, De Novo Sequencing, and Molecular Docking

Li,

Abou-Elsoud,

Chen

et al. 2024

J. Agric. Food Chem.

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“…61 Various studies evidenced that lunasin supplementation to lipopolysaccharide (LPS)-stimulated macrophages resulted in the decrease of pro-inflammatory biomarkers associated with an inhibition of nuclear translocation of the p65 and p50 NF-κB subunits and the protein kinase B-mediated NF-κB pathway. 62–65 This effect is mediated by the interaction between the Arg-Gly-Asp motif present in lunasin with αVβ3 integrin, which is reportedly associated with the activation of inflammatory pathways. 66…”

Section: Discussionmentioning

confidence: 99%

Cell culture models for assessing the effects of bioactive compounds in common buckwheat (Fagopyrum esculentum): a systematic review

Borgonovi,

Iametti,

Speranza

et al. 2024

Food Funct.

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Plant‐derived bioactive peptides: A comprehensive review

Kadam,

Koksel

et al. 2024

Sustainable Food Proteins

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Bioactive peptides that are derived through the enzymatic hydrolysis of proteins have garnered significant attention for their diverse health‐promoting properties. This article delves into the technology behind plant‐derived bioactive peptide production by emphasizing the principles of enzyme hydrolysis. Furthermore, the review highlights the refinement techniques, employing membrane ultrafiltration and various forms of column chromatography to isolate and purify these peptides effectively. The bioactive properties of protein hydrolysates encompass a range of health benefits, including antihypertensive, antioxidant, anti‐diabetic, anti‐obesity, and neuroprotective effects. Additionally, bioactive peptides demonstrate promise in inhibiting cancer cell growth, supporting bone health, and modulating immune responses. These properties hold immense potential for revolutionizing approaches to various health conditions. Continued research in this field promises further insights and breakthroughs, propelling bioactive peptides to the forefront of nutrition and healthcare innovation. The convergence of advanced technology and the multifaceted bioactivity of peptides present an exciting avenue for improving human well‐being and addressing a wide array of health challenges.

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Immunomodulatory effects of seed peptide lunasin in RAW264.7 macrophages in obese microenvironments

Cited by 4 publications

References 45 publications

Identification and Molecular Mechanism of Novel Two-Way Immunomodulatory Peptides from Ovalbumin: In Vitro Cell Experiments, De Novo Sequencing, and Molecular Docking

Identification and Molecular Mechanism of Novel Two-Way Immunomodulatory Peptides from Ovalbumin: In Vitro Cell Experiments, De Novo Sequencing, and Molecular Docking

Cell culture models for assessing the effects of bioactive compounds in common buckwheat (Fagopyrum esculentum): a systematic review

Plant‐derived bioactive peptides: A comprehensive review

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