Immunomodulatory effects of deadLactobacilluson murine splenocytes and macrophages

Abstract

To elucidate the immunomodulation effects of dead lactobacilli, whole cells and gastrointestinal enzymatic hydrolysates of supernatants and precipitates from Lactobacillus paracasei subsp. paracasei NTU 101 and L. plantarum NTU 102 on RAW264.7 macrophages and splenocytes were investigated. Increased NO, COX-2 expression, IL-10 and IL-12 were observed in high-dose precipitates and whole cells of both strains after 24-h stimulation. All of the hydrolysates and whole cells from both strains induced lower pro-inflammatory cytokines (IL-1b and IL-6) than LPS. The supernatants activated cell division to the S phase or promoted advance to the G2/M phase. Regardless of the Lactobacillus strains, higher levels of TNF-a, IL-6, IL-10 and IL-12 in splenocytes were induced by the precipitates. Supernatant of NTU 101 increased the amounts of IFN-g than precipitate in splenocytes. It shows that hydrolysates of NTU 101 induce the proliferations of macrophage and splenocyte and the release of IL-10 and IL-12 cytokines to modulate the innate and adaptive immune systems and inflammatory response.

Safety and mutagenicity evaluation of Vigiis 101 powder made from Lactobacillus paracasei subsp. paracasei NTU 101

“…This type of milk is also useful for prevention of acute gastric ulcers induced by pylorus ligation and acidified ethanol (via prostaglandin E 2 ), and it significantly enhances superoxide dismutase (SOD) activity (Liu et al, 2009). Moreover, NTU 101F milk can attenuate bone loss in ovariectomized (OVX) mice (Chiang and Pan, 2011) and aginginduced bone loss in BALB/c mice and can lower the risk of osteoporosis (Chiang et al, 2012b). In addition, NTU 101F milk can upregulate and downregulate lipolysis and heparin-releasable lipoprotein lipase, respectively, in 3T3-L1 cells and can reduce obesity in Wistar rats fed a high-fat diet (Lee et al, 2013).…”

Lactobacillus paracasei PS23 Delays Progression of Age-Related Cognitive Decline in Senescence Accelerated Mouse Prone 8 (SAMP8) Mice

“…Although LPPS23 has not been investigated, previous studies have suggested that L. paracasei , either alone or mixed with other probiotics, significantly affects the brain and nervous system [ 27 , 28 , 29 ]. Moreover, L. paracasei NTU 101 has been reported to prevent oxidative stress and modulate inflammatory responses [ 30 , 31 ]. Thus, we hypothesized that LPPS23 exhibits similar functions in SAMP8 mice.…”

Immunostimulatory activity of exopolysaccharides from probiotic Lactobacillus casei WXD030 strain as a novel adjuvant in vitro and in vivo

“…So the EPSs derived from microbial are a new candidate adjuvant for vaccines. Lactobacilli, including Lactobacillus casei (L. casei), commonly used probiotic bacteria, show the immunomodulatory properties (Chiang, Liu, Tseng, Mau, & Pan, 2012;Giahi, Elmadfa, Hoseini, & Klein, 2013). EPSs are major components of extracellular polymeric substances and the lactobacilli biofilm matrix (Ciszek-Lenda et al, 2011;Oleksy & Klewicka, 2018).…”