Immunomodulatory Effects of Coated Gold Nanoparticles in LPS-Stimulated In Vitro and In Vivo Murine Model Systems

Moyano, Daniel F.; Liu, Yuanchang; Ayaz, Furkan; Hou, Singyuk; Puangploy, Premsak; Duncan, Bradley; Osborne, Barbara A.; Rotello, Vincent M.

doi:10.1016/j.chempr.2016.07.007

Cited by 49 publications

(45 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similarly, a series of zwitterionic AuNPs accumulated less serum protein absorption on the NP surface (corona free), resulting in lower cellular uptake than cationic AuNPs [48]. NPs bearing a hydrophobic zwitterionic functionality boosted LPS-induced inflammatory outcomes, whereas hydrophilic zwitterionic NPs generated minimal immunology response in vivo (Figure 2.b) [49]. …”

Section: Physicochemical Properties Of Nanoparticles Modulate Innamentioning

confidence: 99%

Effects of engineered nanoparticles on the innate immune system

Liu

Hardie

Zhang

et al. 2017

Seminars in Immunology

Self Cite

207

131

View full text Add to dashboard Cite

Engineered nanoparticles (NPs) have broad applications in industry and nanomedicine. When NPs enter the body, interactions with the immune system are unavoidable. The innate immune system, a non-specific first line of defense against potential threats to the host, immediately interacts with introduced NPs and generates complicated immune responses. Depending on their physicochemical properties, NPs can interact with cells and proteins to stimulate or suppress the innate immune response, and similarly activate or avoid the complement system. NPs size, shape, hydrophobicity and surface modification are the main factors that influence the interactions between NPs and the innate immune system. In this review, we will focus on recent reports about the relationship between the physicochemical properties of NPs and their innate immune response, and their applications in immunotherapy.

show abstract

Section: Physicochemical Properties Of Nanoparticles Modulate Innamentioning

confidence: 99%

Effects of engineered nanoparticles on the innate immune system

Liu

Hardie

Zhang

et al. 2017

Seminars in Immunology

Self Cite

207

131

View full text Add to dashboard Cite

show abstract

“…To probe the effect of surface chemistry on biological systems, inorganic NPs such as AuNPs decorated with different functions groups have been employed ,. However, the heterogeneous polymer coatings on these NPs could not guarantee that only the pure functional group will be presented and studied .…”

Section: Figurementioning

confidence: 99%

Mitigation of Inflammatory Immune Responses with Hydrophilic Nanoparticles

Xie

Yuan

et al. 2018

Angew Chem Int Ed

View full text Add to dashboard Cite

While hydrophobic nanoparticles (NPs) have been long recognized to boost the immune activation, whether hydrophilic NPs modulate an immune system challenged by immune stimulators and how their hydrophilic properties may affect the immune response is still unclear. To answer this question, three polymers, poly(ethylene glycol) (PEG), poly(sulfobetaine) (PSB) and poly(carboxybetaine) (PCB), which are commonly considered hydrophilic, are studied in this work. For comparison, nanogels with uniform size and homogeneous surface functionalities were made from these polymers. Peripheral blood mononuclear cells (PBMCs) stimulated by lipopolysaccharide (LPS) and an LPS-induced lung inflammation murine model were used to investigate the influence of nanogels on the immune system. Results show that the treatment of hydrophilic nanogels attenuated the immune responses elicited by LPS both in vitro and in vivo. Moreover, we found that PCB nanogels, which have the strongest hydration and the lowest non-specific protein binding, manifested the best performance in alleviating the immune activation, followed by PSB and PEG nanogels. This reveals that the immunomodulatory effect of hydrophilic materials is closely related to their hydration characteristics and their ability to resist non-specific binding in complex media.

show abstract

“…Lockman et al [20] demonstrated that cationic carbon NPs exert toxicity at the blood brain barrier while anionic and neutral NPs did not. Furthermore, Moyano et al [21] showed that neutral tetraethylene glycol (TEG) AuNPs do not elucidate inflammatory responses in an in vivo mouse model. Bartneck et al [22] highlighted the fact that charge rather than size affects the capacity of NPs to be trapped.…”

Section: Introductionmentioning

confidence: 99%

Assessment of Charged AuNPs: From Synthesis to Innate Immune Recognition

Rahme

Minassian

Sarkis

et al. 2018

Journal of Nanomaterials

View full text Add to dashboard Cite

Gold nanoparticle (AuNP) physicochemical characteristics, mainly size and charge, modulate their biodistribution, cytotoxicity, and immunorecognition as reported fromin vitroandin vivostudies. While data fromin vitrostudies could be biased by several factors including activation of cells upon isolation and lack of sera proteins in the microenvironment of primary generated cell lines,in vivostudies are costly and time-consuming and require ethics consideration. In this study, we developed a simple and novelin vivo-like method to test for NP immunorecognition from freshly withdrawn human blood samples. AuNPs with a size range of 30 ± 5 nm coated with cationic poly(L-lysine) (PLL) dendrigraft and slightly negative poly(vinyl alcohol) (PVA) were synthesized in water. PLL-capped AuNPs were further coated with poly(ethylene glycol) (PEG) to obtain nearly neutrally charged PEG-AuNPs. Physicochemical properties were determined using zeta potential measurements, UV-Vis spectroscopy, dynamic light scattering (DLS), and scanning electron microscopy (SEM). Gel electrophoretic separation, zeta potential, and DLS were also used to characterize our NPs after human blood plasma treatment. PLL-AuNPs showed similar variation in charge and binding affinity to plasma proteins in comparison with PVA-AuNPs. However, PLL-AuNPs.protein complexes revealed a drastic change in size compared to the other tested particles. Results obtained from the neutrophil function test and pyridine formazan extraction revealed the highest activation level of neutrophils (~70%) by 50 μg/mL of PLL-AuNPs compared to a null induction by PEG- and PVA-AuNPs. This observation was further verified by flow cytometry analysis of polymorphonuclear cell size variation in the presence of coated AuNPs. Overall, ourin vivo-like method, to test for NP immunorecognition, proved to be reliable and effective. Finally, our data supports the use of PEG-AuNPs as promising vehicles for drug delivery, as they exhibit minimal protein adsorption affinity and insignificant charge and size variation once introduced in whole blood.

show abstract

Immunomodulatory Effects of Coated Gold Nanoparticles in LPS-Stimulated In Vitro and In Vivo Murine Model Systems

Cited by 49 publications

References 36 publications

Effects of engineered nanoparticles on the innate immune system

Effects of engineered nanoparticles on the innate immune system

Mitigation of Inflammatory Immune Responses with Hydrophilic Nanoparticles

Assessment of Charged AuNPs: From Synthesis to Innate Immune Recognition

Contact Info

Product

Resources

About