Immunomodulatory effects of a 3.2kDa polypeptide from velvet antler of Cervus nippon Temminck

Zha, Enhui; Li, Xingxia; Li, Dandan; Guo, Xuesong; Gao, Shandian; Yue, Xiqing

doi:10.1016/j.intimp.2013.02.027

Cited by 36 publications

(23 citation statements)

References 15 publications

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“…Their immune function involves humoral mediation, and their number and ratio are important for maintaining stable immune systems in organisms . Previous studies showed that the hydrolytic peptides from wheat, hartshorn, and salmon can increase the number of CD4 + cells and the ratio of CD4 + to CD8 + . Similar to previous results, the three types of HHPs of different molecular weights can increase the proportion of CD4 + and CD8 + , but the HHPs with medium and low molecular weight demonstrate better effects.…”

Section: Discussionsupporting

confidence: 74%

In vivo assessment of immunomodulatory activity of hydrolysed peptides from Corylus heterophylla Fisch

et al. 2015

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Section: Discussionsupporting

confidence: 74%

In vivo assessment of immunomodulatory activity of hydrolysed peptides from Corylus heterophylla Fisch

et al. 2015

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“…Deer antler base contains protein, polypeptide, amino acids, polysaccharides, cholesterol, phosphatidylcholine, etc., and contains a lot of calcium, phosphorus, zinc, iron, manganese, copper, and other trace elements, and is rich in protein substances as its the main pharmaceutical ingredients. Modern medical research results proved that deer antler base improves immunity, offers antiinflammatory and anti-fatigue benefits, and exerts a significant influence on treatment of early breast carbuncle (Zha et al, 2013). Protein polypeptide obtained from hydrolysing deer antler base by signal enzyme could promote phagocyte function, increase the efficacy of T lymphocyte, and enhance immunity (Wang et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Effects of Deer Antler Base Polypeptide on Hormone-induced Mammary Hyperplasia in Mice

Tao

et al. 2018

FSTR

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The objective of this study is to evaluate the effects and therapeutic mechanism of deer antler base polypeptide on mammary hyperplasia in female mice. A trail was conducted on seventy mice which were divided into seven groups including the groups of control, mock, Chinese traditional medicine, and Western medicine, as well as high-dose, medium-dose, and low-dose of polypeptide from deer antler. A combination of oestrogen-progestin induced the mammary hyperplasia. We used an intraperitoneal injection in a 30 d trial and used intragastric administration to treat the disease for a further four weeks. The body weight, organ coefficient, serum hormone level and oxidation level, as well as the pathological section of breast tissue were assessed. Statistical analysis was performed using factor analysis of variance. Compared with the mock group, the high-dose group could increase the serum T-SOD, and GSH-PX activities and thymus coefficient (P < 0.01), decrease the serum MDA concentration and the coefficient of ovary and uterus (P < 0.01). In addition, the high-dose group had reduced levels of E 2 , PRL, and improved P, and LH as confirmed by ELISA to regulate hormone secretion to normal (P < 0.01). The pathological section of breast tissue produced by HE staining showed that the high-dose group reduced the number of inflammatory cells, glandular secretion, the hyperplasia and dilatation of the duct epithelium, and recovered the atrophic alveolar. The results revealed that deer antler base polypeptide has positive effects in the treatment of HMG and the prevention of breast cancer.

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“…Velvet antler polypeptides (VAPs) of sika deer ( Cervus nippon Temminck) are extracts obtained from the traditional Chinese medicine, sika deer velvet antler. VAPs have several biological benefits, including the perfect regeneration of neurons, blood vessels, connective tissue, cartilage and bones ( 1 – 3 ), in addition to immunomodulatory effects ( 4 ). However, their neuroprotective effects in neurodegenerative diseases remain to be reported.…”

Section: Introductionmentioning

confidence: 99%

Protective effects of Cervus nippon Temminck velvet antler polypeptides against MPP+-induced cytotoxicity in SH-SY5Y neuroblastoma cells

Xin

Yang

et al. 2017

Molecular Medicine Reports

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The aim of the present study was to examine the protective effects and mechanism of sika deer (Cervus nippon Temminck) velvet antler polypeptides (VAPs) against MPP+ exposure in the SH-SY5Y human neuroblastoma cell line. MPP+ cytotoxicity and the protective effects of VAPs on the SH-SY5Y cells were determined using an MTT assay. Cell apoptosis and mitochondrial membrane potential were detected using Hoechst 33342 and Rhodamine123 staining, respectively. Endoplasmic reticulum (ER) stress-related reactive oxygen species (ROS) production in the SH-SY5Y cells was detected using 2′,7′-dichlorodihydrofluorescein diacetate fluorescent probes. The expression levels of proteins, including caspase-12, glucose regulated protein 78 (GRP78), CCAAT/enhancer binding protein homologous protein (CHOP) and phosphorylated c-Jun N-terminal kinase (p-JNK) were detected using western blot analysis. The results showed that the half inhibitory concentration of MPP+ at 72 h was 120.9 µmol/l, and that 62.5, 125, and 250 µg/ml concentrations of VAPs protected the SH-SY5Y cells under MPP+ exposure. When exposed to 120.9 µmol/l MPP+, changes in cell nucleus morphology, mitochondrial membrane potential and intracellular ROS were observed. VAPs at concentrations of 62.5, 125, 250 µg/ml reduced this damage. Western blot analysis showed that protein expression levels of caspase-12, GRP78 and p-JNK were upregulated in the SH-SY5Y cells exposed to 120.9 µmol/l MPP+ for 72 h. In addition, 62.5, 125, and 250 µg/ml VAPs downregulated the expression levels of caspase-12 and p-JNK in a concentration- dependent manner, particularly the p-JNK pathway. The effects of VAPs on GRP78 and CHOP were weak. In conclusion, MPP+-induced SH-SY5Y cell death may be linked to ER stress. VAPs prevented MPP+-induced SH-SY5Y cell death by affecting the p-JNK pathway and caspase-12-mediated apoptosis. These findings assist in understanding the mechanism underlying the protective effect of VAPs on neurons.

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Immunomodulatory effects of a 3.2kDa polypeptide from velvet antler of Cervus nippon Temminck

Cited by 36 publications

References 15 publications

In vivo assessment of immunomodulatory activity of hydrolysed peptides from Corylus heterophylla Fisch

In vivo assessment of immunomodulatory activity of hydrolysed peptides from Corylus heterophylla Fisch

Effects of Deer Antler Base Polypeptide on Hormone-induced Mammary Hyperplasia in Mice

Protective effects of Cervus nippon Temminck velvet antler polypeptides against MPP+-induced cytotoxicity in SH-SY5Y neuroblastoma cells

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