Immunomodulatory effect of Rhaphidophora korthalsii on mice splenocyte, thymocyte and bone marrow cell proliferation and cytokine expression

Abstract

Rhaphidophora korthalsii methanol extract was previously found as a potential in vitro immunomodulating agent. In this study, the in vitro immunomodulatory activity of R. korthalsii methanol extract on mice splenocyte were quantified through evaluating T/NK cell population, cytokine expression (IL-2 and IFN-) and cytotoxicity against Yac-1 cell. In vitro MTT proliferation study showed that R. korthalsii methanol extract stimulate significant proliferation of splenocyte, thymocyte and bone marrow cell at 25 g/ml after 72 h incubation as compared to rIL-2. Besides, 25 g/ml of extract also enhanced T-helper 1 cytokine (IL-2 and IFN-) secretion, natural killer cell (NK1.1 + CD3 -) population and cytotoxicity against natural killer sensitive Yac-1 leukemic cell line. It was concluded that R. korthalsii methanol extract is a potential immunomodulator agent that could activate the natural killer cell.

Immunotoxicity and proliferation of human peripheral blood mononuclear cells of HIV-positive patient after treatment with aqueous extract of polyherbal formulation

“…Our finding is in line with the findings of Swee et al (2011) who reported that Rhaphidophora korthalsii methanol extract induced proliferation and activation of PBMCs, which in turn induce apoptosis of effector cancer cell (K562).…”