Immunomodulatory effect of Canova medication on experimental infection

Pereira, Wlademir; Lonardoni, Maria Valdrinez Campana; Grespan, Renata; Caparroz-Assef, Silvana Martins; Cuman, Roberto Kenji Nakamura; Bersani-Amado, Ciomar Aparecida

doi:10.1016/j.jinf.2004.09.009

Cited by 52 publications

(50 citation statements)

References 30 publications

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“…CA stimulates an increase of endosomal/lysosomal system, increases macrophages phagocytic activity of non-infective microorganisms (Saccharomyces cerevisiae and Trypanosoma cruzi epimastigotes) and diminishes phagocytic activity of infective microorganisms T. cruzi trypomastigotes and Leishmania amazonensis [Lopes et al, 2006]. CA modulatory effects were also observed in experimental infection both in vivo and in vitro by L. amazonensis, controlling progression of infection and limiting it dissemination [Pereira et al, 2005] and also in experimental infection with Paracoccidioides brasiliensis in a way that depends, at least in part, on NO production [Takahachi et al, 2006]. Sarcoma 180-bearing mice treated with CA showed reduction in sarcoma size and a significant infiltration of lymphoid cells, granulation tissue and fibrosis surrounding the tumor.…”

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confidence: 97%

Gene expression profiling of macrophages following mice treatment with an immunomodulator medication

Oliveira

Góes

et al. 2008

J of Cellular Biochemistry

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Canova (CA) is a complex homeopathic medication used in diseases where the immune system is depressed. Previous studies demonstrated that it is neither toxic nor mutagenic and activates macrophages. We now evaluate CA effects on cytokine production and gene expression from mice macrophages. The global view of changes in expression of genes with known functions can provide a vivid picture of the way in which cell adapts to a changing environment or a challenge. We found a decrease in IL-2 and IL-4 production and a differential expression in 147 genes from CA group. These genes are mainly involved in transcription/translation, cell structure and dynamics, immune response, cytoprotection, enzymatic process, and receptors/ligands. With gene expression analysis we state that this medication provokes a reaction that involves alterations in gene expression profile mainly in the ones involved with macrophages activation, corroborating the laboratorial research and the clinical data.

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confidence: 97%

Gene expression profiling of macrophages following mice treatment with an immunomodulator medication

Oliveira

Góes

et al. 2008

J of Cellular Biochemistry

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“…The animals were treated with Canova ® medicine (Canova of Brazil) (0.2 mL day -1 , intra-gastric route) (Pereira et al, 2005) or Itraconazole as reference drug, (Sporanox …”

Section: Treatmentmentioning

confidence: 99%

“…Some recent experimental studies have shown that the mechanism of action of the compound is probably due to the increase in the immune response through macrophage activation (Piemonte and Buchi, 2002). In vitro studies, Canova promoted the decrease of the production of α-Tumour Necrosis Factor (TNF-α) (Piemonte and Buchi, 2002), stimulation of leishmanicidal activity, and the production of nitric oxide in elicited macrophages (Pereira et al, 2005), suggesting that this medicine might act on some immune response mediators. Paracoccidioidomycosis (PCM) is a granulamatous systemic disease, caused by the fungus Paracoccidioides brasiliensis, and it is geographically limited to Latin American countries (Restrepo, 1985).…”

Section: Introductionmentioning

confidence: 99%

Biochemical responses in mice experimentally infected with Paracoccidioides brasiliensis and treated with Canova

Takahachi

Maluf

Svidzinski

et al. 2006

Braz. arch. biol. technol.

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“…(Pereira et al, 2005;Barbieri et al, 1993). A análise do efeito antiparasitário foi realizada em condições onde estas amastigotas purificadas fossem capazes de se diferenciarem em formas promastigotas .…”

Section: Vetoresunclassified

“…Camundongos BALB/c foram infectados com 1x10 resultado final foi obtido pela subtração da espessura da pata com inoculação de parasitas da espessura da pata controle (M199) (Pereira et al, 2004).…”

Section: Efeito Da Associação Tub-nbmpr Na Evolução Da Lesão De Leishunclassified

Ação do análogo de purina tóxico tubercidina em Leishmania ssp.

Aoki¹

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AgradecimentosAo meu orientador, Prof. Dr. Paulo Cotrim pela oportunidade e confiança dada para a realização deste trabalho, pelos ensinamentos e paciência transmitidos durante esses anos. Gene identification associated with drug resistance has contributed to a better understanding of the mechanism of action of anti parasitic compounds.Using transfection and over-expression selection strategy we isolated two loci related with the resistance of tubercidin (TUB), a toxic analog purine. In the first locus we identified an ortholog of the TOR gene (TOxic nucleoside Resistance) in L. (L.) major (TOR-Lm), capable to render wild cells resistance to TUB after transfection and over-expression. Chromosomal location and identification of the second locus was done, but functional tests in the presence of TUB were not as significant as those obtained after TOR-Lm transfection. In the second part of this work, we evaluate the effectiveness of the association of TUB with an inhibitor specific to the mammals nucleoside transport, as nitrobenzylthioinosine (NBMPR), aimed at reversing the TUB toxicity only on the host. We first demonstrate that TUB has a potent anti-parasitic action in cultures of Leishmania spp. Then, we discuss the capacity of the NBMPR inhibitor to protect infected macrophages from the toxic effects of TUB.

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Immunomodulatory effect of Canova medication on experimental infection

Cited by 52 publications

References 30 publications

Gene expression profiling of macrophages following mice treatment with an immunomodulator medication

Gene expression profiling of macrophages following mice treatment with an immunomodulator medication

Biochemical responses in mice experimentally infected with Paracoccidioides brasiliensis and treated with Canova

Ação do análogo de purina tóxico tubercidina em Leishmania ssp.

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